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ProteomicsProteome-wide analysis of SUMO2 targets in response to pathological DNA replication stress in human cells
SUMOylation is a form of post-translational modification involving covalent attachment of SUMO (Small Ubiquitin-like Modifier) polypeptides to specific lysine residues in the target protein. In human cells, there are four SUMO proteins, SUMO1–4, with SUMO2 and SUMO3 forming a closely related subfami...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-28 | MSV000080699 | MassIVE
GenomicsGenome-wide mapping of early replication fragile sites (ERFS)
DNA double strand breaks (DSBs) in B lymphocytes are thought to arise stochastically during replication (S phase) or as a result of targeted DNA damage by activation induced cytidine deaminase (AID) in G1. Here we identify a novel class of recurrent, early replicating and AID independent DNA lesi...
ORGANISM(S): Mus musculus 
2013-02-04 | E-GEOD-43504 | biostudies-arrayexpress
ProteomicsO-GlcNAcylation of RPA2 at S4/S8 antagonizes phosphorylation and regulates checkpoint activation during replication stress
O-linked N-acetylglucosamine (O-GlcNAc) is the only mono-saccharide modification occurring in the cytoplasm, nucleus and mitochondria. Recent advent of the mass spectrometry technology has enabled identification of abundant O-GlcNAc transferase (OGT) substrates in diverse biological processes, such ...
ORGANISM(S): Homo Sapiens 
PXD053321 |
ProteomicsRPA promotes processive ssDNA digestion by Dna2 via their bimodal interaction in Saccharomyces cerevisiae
Single-stranded DNA (ssDNA) widely exists as intermediates in DNA metabolic pathways. The ssDNA binding protein, RPA, not only protects the integrity of ssDNA, but also directs the downstream factor that signals or repairs the ssDNA intermediate. However, it remains unclear how these enzymes/factors...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-22 | PXD028637 | Pride
Ssdna Saccharomyces cerevisiae Dna2 Rpa 
TranscriptomicsSub1 and RPA associate with RNA Polymerase II at different stages of transcription
Single stranded DNA binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. Quantitative proteomic analysis of RNA polymerase II (RNApII) pre-initiation complexes (PICs) identified Sub1 and the Replication Protein A complex (RPA), both ...
ORGANISM(S): Saccharomyces cerevisiae 
2011-09-26 | E-GEOD-32416 | biostudies-arrayexpress
ProteomicsBreak-induced replication is enhanced by a phospho-activated RPA-binding module in Pol32
Break-induced replication (BIR) mediates single-ended DNA double-strand break (DSB) repair. Upon strand invasion into a homologous repair template, BIR is catalysed by a minimal replisome comprising PCNA, DNA polymerase delta, and the Pif1 helicase. Here, we identify an interaction between Polymeras...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2026-06-15 | PXD074712 | Pride
Cdc5 Break-induced replication Vanquish neo uhplc Ddk Orbitrap exploris 480 Pol32 Cka2 Saccharomyces cerevisiae 
ProteomicsPHOSPHORYLATION-DEPENDENT ASSOCIATION OF WRN WITH RPA IS REQUIRED FOR RECOVERY OF REPLICATION FORKS STALLED AT SECONDARY DNA STRUCTURES
The WRN protein mutated in the premature aging disorder Werner syndrome is vital for metabolism of perturbed replication forks. Replication Protein A (RPA) robustly enhances WRN helicase activity in specific cases when tested in vitro. However, the significance of RPA-binding to WRN in vivo has rema...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000095749 | MassIVE
ProteomicsThe Bloom Syndrome Complex Senses RPA-Coated Single-Stranded DNA to Restart Stalled Replication Forks
The Bloom syndrome helicase BLM interacts with topoisomerase IIIa (TOP3A), RMI1 and RMI2 to form the BTR complex, which dissolves double Holliday junctions to produce non-crossover products during homologous recombination (HR). BLM also promotes DNA-end resection and stalled replication fork restart...
ORGANISM(S): Homo sapiens (Human) 
2021-01-05 | PXD018322 | Pride
Bloom syndrome Dna damage response Blm Rpa Homologous recombination Replication stress 
ProteomicsMechanism of RPA phosphocode priming and tuning by Cdk1/Wee1 signaling circuit
Replication protein A (RPA) is a heterotrimeric single-strand DNA binding protein that is integral to DNA metabolism. Segregation of RPA functions in response to DNA damage is fine-tuned by hyperphosphorylation of the RPA32 subunit that is dependent on Cyclin-dependent kinase (Cdk)-mediated priming ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-12 | PXD068080 | Pride
Rpa domain configurations Rpa phosphorylation Cdk1 Wee1 Cell cycle 
TranscriptomicsImpaired DNA damage metabolism promotes autoimmunity in TREX1 deficiencyÂ
Constitutive low level DNA damage is linked to innate immune activation. Hierarchical clustering of over 9000 transcripts revealed remarkably similar profiles in a patient with lupus erythematosus and a patient with AGS with up-regulation of genes involved in DNA damage signaling, p53-inducible gene...
ORGANISM(S): Homo sapiens 
2016-05-30 | E-GEOD-59233 | biostudies-arrayexpress
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