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Proteomics13C6-Lysine-labelled mouse serum N-terminomics
To investigate the putative lifetime of proteolytically cleaved protein fragments in mouse serum, wildtype mice were fed with 13C6-Lysine for 1 or 2 weeks and the incorporation of 13C6-Lysine into proteins/protein fragments was investigated to achieve lifetime estimations for the fragment(s') genera...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-12 | PXD051273 | Pride
Mouse Serum Hunter Degradome N-termini 13c6-lysine 
ProteomicsProteomic analysis of cultured primary urinary cells from M. Fabry Patients
We analyzed the proteome of primary urinary cells from patients with M. Fabry as compared to primary urinary cells from healthy control samples
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD007081 | Pride
Human Puc Fabry's diseaes 
ProteomicsTripartite separation of glomerular proteomes from reporter-free mice
Renal blood filtration occurs in a functional unit called the glomerulus. The resident cell types comprise the filtration barrier, namely podocytes, mesangial cells and glomerular endothelial cells. Here we introduce a glomerular cell isolation protocol, which enables the separation of these three c...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-12 | PXD024481 | Pride
Mouse kidney glomerulus endotheliall mesangial 
ProteomicsMurine kidney glomeruli phosphorylation
Isolated murine kidney glomeruli were lysed in buffer containing 8M urea, and proteins were reduced and alkylated. Proteins were digested with trypsin. After reverse-phase chomatrography, peptides were fractionated using strong cation exchange chromatography. Phosphopeptides were enriched using IMAC...
ORGANISM(S): Mus musculus (Mouse) 
2016-05-09 | PXD000266 | Pride
Mouse Podocytes Kidney Glomerular epithelial cell Phosphoproteomics 
ProteomicsLC MS/MS of kidney organoids during differentiation from day 21 to 29
Kidney organoids are a valuable and innovative model to understand genetic diseases, kidney development and transcriptomic dynamics. However, their proteome has not been analyzed so far. Here, we analyzed the organoid proteome trajectory during differentiation. Genes involved in podocytopathies and ...
ORGANISM(S): Homo sapiens (Human) 
2023-05-12 | PXD029716 | Pride
Shotgun lc ms/ms Differentiation Maturation Human kidney organoids 
ProteomicsTargeted proteomics analysis of cortical collecting ducts with Pendrin knockout
We analyzed microdissected cortical collecting ducts of pendrin knockouts using a sensitive targeted proteomics approach proteomics approach.
ORGANISM(S): Mus musculus (Mouse) 
2022-04-04 | PXD018791 | Pride
Pendrin Kidney Ccd 
ProteomicsProteomic analysis of kidney organoid supernatant during TNFa stimulation
Kidney organoids are a valuable and innovative model to understand genetic diseases, kidney development and transcriptomic dynamics. However, their proteome has not been analyzed so far. Here, we analyzed the organoid proteome after treatment of organoids with 5ng/mL TNFalpha for 24h and 48h compare...
ORGANISM(S): Homo sapiens (Human) 
2023-05-12 | PXD029696 | Pride
Tnfalpha Cytokine Shotgun lc ms/ms Human kidney organoids Tnfa 
ProteomicsProteome analysis of kidney organoid cells during TNFa stimulation
Kidney organoids are a valuable and innovative model to understand genetic diseases, kidney development and transcriptomic dynamics. However, their proteome has not been analyzed so far. Here, we analyzed the organoid proteome after treatment of organoids with 5ng/mL TNFalpha for 24h and 48h compare...
ORGANISM(S): Homo sapiens (Human) 
2023-05-12 | PXD029718 | Pride
Tnfalpha Cytokine Shotgun lc ms/ms Human kidney organoids Tnfa 
ProteomicsL-Lysine treated rats' kidney shotgun proteome
Hypertension and kidney disease, two related, common, and severe disease entities have been repeatedly associated with genomic variants and metabolic alterations of lysine metabolism. Here, we developed a stable isotope labeling strategy compatible with untargeted metabolomics acquisition to investi...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-06-01 | PXD029232 | Pride
Hypertension Rat Kidney L-lysine 
ProteomicsUntargeted proteomics analysis of cortical collecting ducts of pendrin knockout mice
We compared the proteome of isolated pendrin knockout mice using a data-dependent proteomics acquisition protocol.
ORGANISM(S): Mus musculus (Mouse) 
2022-04-04 | PXD018792 | Pride
Pendrin Kidney Cortical collecting duct 
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