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Proteomics13C6-Lysine-labelled mouse serum N-terminomics
To investigate the putative lifetime of proteolytically cleaved protein fragments in mouse serum, wildtype mice were fed with 13C6-Lysine for 1 or 2 weeks and the incorporation of 13C6-Lysine into proteins/protein fragments was investigated to achieve lifetime estimations for the fragment(s') genera...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-12 | PXD051273 | Pride
Mouse Serum Hunter Degradome N-termini 13c6-lysine 
MetabolomicsChlorophyll biosynthesis under the control of arginine metabolism.

In natural environments, photosynthetic organisms adjust their metabolism to cope with the fluctuating availability of combined nitrogen sources, a growth-limiting factor. For acclimation, the dynamic degradation/synthesis of tetrapyrrolic pigments, as well as of the amino acid arginine, is ...

2024-03-12 | MTBLS8336 | MetaboLights
ProteomicsSILAC Phospho-proteomics: Trypanosoma brucei
Pseudokinases can modulate activity of protein kinases, among other modes of influencing biological pathways. We tested a hypothesis that knockdown of Tb427tmp.160.4770 (a Pseudokinase) perturbs the Phospho-proteome of T. brucei. To discover changes in the trypanosome phospho-proteome, stable isoto...
ORGANISM(S): Trypanosoma brucei 
2023-03-11 | PXD034516 | Pride
Lc-msms Silac Trypanosoma brucei 
ProteomicsComprehensive proteomic analysis of autophagosomes derived from Leishmania infected macrophages
Autophagy has been implicated as a host defense mechanism against intracellular pathogens. However, certain intracellular pathogens such as Leishmania can manipulate the host’s autophagy to promote their survival. Recently, our findings regarding the regulation of autophagy by Leishmania donovani in...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037057 | Pride
Parasitic infection Autophagy Autophagosome Leishmania donovani Host-pathogen interactions Lcmsms 
Proteomics786-O ubiquitiome - Loss of BAP1 Results in Growth Inhibition and Enhances Mesenchymal-Epithelial Transition in Kidney Tumor Cells
786-O WT and BAP1-KO cells were cultured in heavy (13C6-L-arginine, 13C6-L-lysine, Cambridge Isotope Lab) and light (unlabeled L-arginine and L-lysine) SILAC RPMI-1640 medium (Thermo Fisher Scienific), respectively. After more than 10 passages, cells were lysed with 8 M urea and 5 mg protein from ea...
ORGANISM(S): Homo sapiens (Human) 
2019-04-18 | PXD013127 | Pride
Ccrcc 786-o ubiquitiome 
ProteomicsHuman Sumoylome (SUMO-2) upon bacterial infection
Given the role of SUMOylation in pathogenic infection, we wanted to evaluate the changes to the host SUMO-2 subproteome during Shigella infection. HeLa cells overexpressing TAP-SUMO-2 or TAP-empty were used in conjunction with SILAC (Stable Isotope Labeling of Amino acids in Culture) (Golebiowski ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-19 | PXD001100 | Pride
Human Sumo-2 Shigella flexneri Hela Tap tag 
ProteomicsProteomic and phosphoproteomic data of colorectal cancer tissues and cells for Chromosome-Centric Human Proteome Project
Protein extraction and proteolytic digestion were performed using a Filter-Assisted Sample Preparation (FASP) protocol. In case of phosphopeptide enrichment immobilized Fe(III) affinity chromatography (Fe-IMAC) was performed. The peptides were fractionated using an HPLC system fitted with an SCX col...
ORGANISM(S): Homo sapiens (Human) 
2013-01-11 | PXD000089 | Pride
Phosphopeptides Human colon normal tissues Human colorectal cancer tissues Itraq 
ProteomicsBacillus subtilis acetylome and succinylome
The two major acyl-modifications on lysine residues, acetylation and succinylation, were analyzed in the gram-positive model bacterium Bacillus subtilis. To identify and quantify the changes in acetylation and succinylation in response to the carbon source, a stable isotope labeling by amino acids i...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2015-07-02 | PXD001615 | Pride
Acetylome Lc-msms Succinylome Bacillus subtilis 
ProteomicsPulsed SILAC quantitative proteomics profiling to understand the protective role of ginsentide TP1 against hypoxia.
This project used a Pulsed SILAC (pSILAC) proteomics approach to pulsed/chase the incorporation of stable isotope-labeled lysine (13C6 and 15N2 L-lysine) and arginine (13C6 L-arginine) into newly synthesized proteins and profiled de novo protein synthesis in endothelial cells (HUVEC-CS) subjected to...
ORGANISM(S): Homo Sapiens (human) 
2024-02-08 | PXD039938 | JPOST Repository
Proteomicsdynamic SILAC CGR8 mESC DMSO/CHX
mESCs were cultured at least 2 weeks in medium medium: N2B27 for SILAC (DMEM/F12 for SILAC (AthenaES), Neurobasal for SILAC (AthenaES), Sodium Pyruvate (40 mg/mL), N2 (1X), B27 (0.5X), Pen/Strep (1%), L-glutamine (2 mM), beta-mercaptoethanol (50 µM)) + 2i/LIF supplemented with (medium) 13C6 15N4 L-a...
ORGANISM(S): Mus musculus (Mouse) 
2025-10-07 | PXD061306 | Pride
Cgr8 Dsilac Mesc 
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