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ProteomicsLck promotes Zap70-dependent LAT phosphorylation by bridging Zap70 to LAT
T cell antigen receptor (TCR) signaling depends upon the kinases Lck and Zap70. Lck phosphorylates the TCR, facilitating Zap70 recruitment to the stimulated TCR. Lck also phosphorylates Zap70, relieving its auto-inhibition and activating its catalytic domain. Zap70 then phosphorylates the critical a...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD008258 | Pride
Jurkat t cell line Lat Tcr signaling Mice Lck Zap70 
ProteomicsPhosphotyrosine proteomics reveals novel Zap70 and Itk pathway targets downstream of TCR and CAR in Jurkat T cells
ζ-associated protein of 70 kDa (Zap70) and interleukin-2-inducible T cell kinase (Itk) propagate the primary and CD28-integrated phosphotyrosine (pY) signalling, respectively, to achieve full T cell activation. Despite their canonical roles in T cell activation, our understanding of how each kinase ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-03 | PXD069485 | Pride
ProteomicsVav1 regulates T cell activation through a feedback mechanism and crosstalk between the T cell receptor and CD28 in CD3-stimulated T cells
Vav1, a Rac/Rho guanine nucleotide exchange factor and a critical component of the T cell receptor (TCR) signaling cascade, is rapidly tyrosine phosphorylated in response to T cell activation. Previous studies using Vav1 knockout models have demonstrated its importance in TCR signaling. Although Vav...
ORGANISM(S): Homo sapiens (Human) 
2016-05-31 | PXD002022 | Pride
T cell receptor Tyrosine phosphorylaiton Vav1 
ProteomicsPhosphoproteomic analysis of successive Jurkat CD19-CAR generations reveals TCRζ-driven signalling
Although chimeric antigen receptor (CAR) T cell therapy has revolutionised individualised cancer therapies for relapsed/refractory lymphomas, low long-term retention due to basal signalling (antigen-independent activation in the absence of cognate antigen) and off-target toxicity limit the broad app...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD065669 | Pride
ProteomicsThe catalytic activity of the kinase Zap-70 mediates basal signaling and negative feedback of the T cell receptor pathway
T cell activation must be properly regulated to ensure normal T cell development and effective immune responses to pathogens and transformed cells, while avoiding autoimmunity. Our knowledge of the mechanisms controlling the fine-tuning of T cell receptor (TCR) signaling and T cell activation is sti...
ORGANISM(S): Homo sapiens (Human) 
2016-05-31 | PXD002023 | Pride
T cell Phosphoproteomics Lc-ms/ms 
ProteomicsSILAC proteomics of cancer-associated fibroblasts (CAFs) and cancer cells
To study bi-directional signaling between cancer-associated fibroblasts (CAFs) and cancer cells, we used stable isotope labeling by amino acids in cell culture (SILAC) to determine changes in tyrosine phosphorylation upon direct contact of the two cell types.
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD003574 | Pride
Cancer-associated fibroblasts Cancer cells Silac 
ProteomicsDeep phosphotyrosine characterization of mouse primary T cells using Broad Spectrum Optimization of Selective Triggering
Sequencing the tyrosine phosphoproteome using MS-based proteomics is challenging due to the low abundance of tyrosine phosphorylation in cells, a challenge compounded in scarce samples like primary cells or clinical samples. The broad-spectrum optimization of selective triggering (BOOST) method was ...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-08 | PXD053863 | Pride
T cell Mus musculus Tmt 
ProteomicsCAR T cells inhibit B cell activating phosphorylation in their targets
Adoptive cell therapy, a subset of cancer immunotherapy, is collection of therapeutic approaches which aim to redirect the immune system by reprogramming patient T-cells to target antigenic molecules differentially and specifically expressed in certain cancers. One promising immunotherapy technique ...
ORGANISM(S): Homo sapiens (Human) 
2022-01-28 | PXD028109 | Pride
Cart signaling Phosphoproteomics Phosphotyrosine 
ProteomicsHighly reproducible improved label-free quantitative analysis of cellular phosphoproteome by optimization of LC-MS/MS gradient and analytical column construction
Expanding the sequencing depth of the peptides showing a statistically significant quantitative change arising from a biological stimulation is critical. Here we demonstrate that optimization of LC gradient and analytical column construction can reveal over 30,000 unique peptides and 23,000 phosphop...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD005650 | Pride
Human T cell Acute leukemia Q exactive Phosphoproteomics Label free 
ProteomicsThe phosphatases TCPTP, PTPN22, and SHP1 play unique roles in T cell phosphotyrosine maintenance and feedback regulation of the TCR
The protein tyrosine phosphatases (PTPs) TCPTP, PTPN22, and SHP1 are critical regulators of the activating phophotyrosine (pY) site on the initiating T cell kinase, LckY394, but the broader implications of these phophatases in T cell receptor (TCR) signalling and T cell biology remain unclear. By co...
ORGANISM(S): Homo sapiens (Human) 
2025-08-01 | PXD058624 | Pride
Shp1 Phosphoproteomics Tcptp Ptpn22 T cell signaling 
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