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ProteomicsPhosphoproteomics sample preparation impacts biological interpretation of signalling responses
Quantitative data-dependent acquisition phosphoproteomics (DDAP) has revolutionised cell signaling studies. However, the impact of the specific DDAP sample preparation method on the phosphoproteome quantified is unclear as each study quantifies only a fraction of the phosphoproteome. Here, we compar...
ORGANISM(S): Mus musculus (Mouse) 
2021-12-25 | PXD025376 | Pride
Phosphoproteomics Tio2 Mouse High-throughput phosphoproteomics Scxphos Sample preparation Highphos Comparative analysis 
ProteomicsQuantitative phosphoproteomics to unravel the cellular response to chemical stressors with different modes of action
Cells activate various stress response pathways when exposed to chemicals that can damage cellular macromolecules and organelles. Whether different chemical stressors activate common and/or stressor-specific pathways and to what extent is largely unclear. Here, we used quantitative phosphoproteomics...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-23 | PXD012559 | Pride
Phosphoproteomics Topbp1 Mdc1 Post-translational modification Mass spectrometry Cyclosporinea Mouse Biological Phosphorylation Lc-msms Mescs Atr Atm Silac Signal transduction Diethylmaleate Etoposide Cisplatin Rad51 
ProteomicsMouse ESC LC-MSMS Ionising radiation
Dose-dependent (phospho) proteome responses of mouse embyronic stem cells to ionising radiation. Quantitative SILAC- and mass spectrometry-based proteomics and phosphoproteomics experiments were performe at half an hour (0.5h) and four hours (4h) after exposure to LD (0.1 Gy) and high doses (HD; 1 G...
ORGANISM(S): Mus musculus (Mouse) 
2022-03-18 | PXD025343 | Pride
Reactive oxygen species Transporter Phosphoproteome Ionising radiation Mouse Mitochondria Proteome Dna damage response 
ProteomicsThe dynamics of Atm signalling dictate cellular fate decisions after DNA damage
DNA damage activates diverse cellular responses – either protective or deleterious –that ultimately promote or inhibit proliferation. How the distinct responses conferring crucial cell fate decisions are chosen is unclear. Using a systems approach, we demonstrate that the dynamic features of Atm...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-18 | PXD013620 | Pride
Phosphoproteomics Mdc1 Post-translational modification Mass spectrometry Mouse Ionizing radiation Biological Phosphorylation Lc-msms Mescs Atm Silac Signal transduction Chek2 
ProteomicsHuman CRC cell line baseline phosphoproteomics
There is a need for robust phosphopeptide enrichment methods to allow signaling network analysis in cancer cell lines and tissues with minimal fractionation. With recent instrument developments thousands of unique phosphopeptides can be detected by single-shot LC-MS/MS. However, successful phosphopr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080788 | MassIVE
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