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ProteomicsHadBD dehydratase from Mycobacterium tuberculosis FAS-II: a singular structure for a unique function
Mycolic acids (MAs) are a unique class of lipids that are essential for viability, virulence and persistence of Mycobacterium tuberculosis (Mtb). Therefore, enzymes involved in MAs biosynthesis represent important class of drug targets. We previously showed that (3R)-hydroxyacyl-ACP dehydratase (HAD...
ORGANISM(S): Mycobacterium bovis BCG str. Pasteur 1173P2 
2024-03-21 | PXD043977 | Pride
(3r)-hydroxyacyl-acp dehydratase Structure-function relationships. Mycolic acids Hydratase 2 Mycobacterium tuberculosis Hotdog fold Crystal structure Catalytic subunit Substrate specificity Fatty acid synthase type ii system 
ProteomicsFranco-Swiss HPP project (Chromosomes 2 and 14)
The dataset is a concatenation of nanoLC-MS/MS analyses performed on different sample types/sample preparations of human proteins on various instrumental platforms in the laboratory. A franco-swiss collaboration to identify missing human proteins on chromosomes 2 and 14 in the frame of the Human Pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080273 | MassIVE
ProteomicsOverexpression of the mycobacterial PknB Ser/Thr kinase uncovers its global regulatory role in mycolic acids biosynthesis and trafficking
Comparison of the global phospho-proteomes of Mycobacterium smegmatis wild type, and over-expressing the protein kinase PknB from Mycobacterium tuberculosis or its enzymatically inactive K40M isoform.
ORGANISM(S): Escherichia coli 
2021-08-30 | PXD012553 | Pride
Mycobacterium smegmatis phosphoproteomics nanohlpc-ms/ms 
ProteomicsMass spectrometry analysis of cleavage sites for different protease allergens in human IL-33
The cytokine interleukin-33 (IL-33) is an epithelial alarmin with critical roles in allergic inflammation and type 2 immunity. The project aims at the characterization of the direct cleavage of IL-33 by allergen proteases, resulting in its activation, and in the subsequent induction of type 2 cytoki...
ORGANISM(S): Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) 
2018-03-14 | PXD006081 | Pride
Peptide mapping Interleukin-33 Human Protease processing Lc-ms/ms 
ProteomicsDurum Wheat Proteome Changes under Marine and Fungal Biostimulant Treatments
Durum wheat requires high nitrogen inputs to obtain the high protein concentration necessary to satisfy pasta and semolina quality criteria. Optimizing plant nitrogen use efficiency is therefore of major importance for wheat grain quality. Here, we studied the impact on grain yield, protein concentr...
ORGANISM(S): Triticum aestivum (Wheat) 
2019-03-29 | PXD012469 | Pride
Proteomics Protein composition Label-free quantification Biostimulant Durum wheat 
ProteomicsAnalysis of Tim-3 interactome in primary mouse CD4+ T cells
We analyzed the interactome of the Tim-3 (HAVCR2) protein, using an affinity purification method based on the the endogenous expression of a One-Strep-tagged (OST) version of Tim-3 in an engineered mouse model, and affinity purification of the protein from expanded primary CD4+ T cells. Affinity pu...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-30 | PXD020156 | Pride
Ap-ms Tcr Primary cd4+ t cell Interactome Signalosome 
ProteomicsAnalysis of SLP-76 interactomes of human primary CD4+ T cells in presence or absence of Dasatinib treatmentAnalysis of SLP-76 interactomes of human primary CD4+ T cells in presence or absence of Dasatinib treatment
Using a CRISPR/Cas9-based approach, we engineered human primary CD4+ T cells in which the bait protein SLP76 was tagged with an affinity Twin-Strep-tag (OST). Through affinity purification coupled with quantitative mass spectrometry, this system allows determining the composition and dynamics of the...
ORGANISM(S): Homo sapiens (Human) 
2022-01-28 | PXD028939 | Pride
Ap-ms Tcr Dasatinib Slp76 Primary cd4+ human t cell Crispr/cas9 Interactome Ost tag 
ProteomicsProteome of long-term expanded human CD4+ and CD8+ T cells
We analyzed the total proteome of CD4+ and CD8+ T cells isolated from human peripheral blood mononuclear cells (PBMC), and cultured to perform a CRISPR/CAS9 edition of their genome, in order to introduce an OST sequence at the C-terminus of proteins of interest (SLP76 or ZAP70, n=3 biological replic...
ORGANISM(S): Homo sapiens (Human) 
2022-01-10 | PXD025174 | Pride
Crispr/cas9 Whole cell lysate Proteome Primary cd4+ and cd8+t cell 
ProteomicsAnalysis of the interactomes of ZAP70, CD247 and SLP76 upon stimulation of murine CD8+ T cells with ligands of different affinity to the TCR
T cells have the remarkable ability to sense and discriminate between a wide range of antigenic peptides bound to major histocompatibility complex molecules (pMHC). Here, we use interactomics to monitor in a time dependent manner the early molecular events taking place upon stimulation of murine CD8...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-05 | PXD029974 | Pride
Ap-ms Tcr Slp76 Cd3z Primary cd8+ t cell Ot-i mouse Zap70 Interactome Cd247 Ost tag 
ProteomicsAAnalysis of LAT, SLP76, VAV1 and ZAP70 interactomes of expanded human CD4+ and CD8+ T cells nalysis of LAT, SLP76, VAV1 and ZAP70 interactomes of expanded human CD4+ and CD8+ T cells
Using a CRISPR/Cas9-based approach, we engineered human primary CD4+ and CD8+ T cells in which a bait protein (LAT, SLP76, VAV1 or ZAP70) was tagged with an affinity Twin-Strep-tag (OST), with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosom...
ORGANISM(S): Homo sapiens (Human) 
2022-01-10 | PXD024820 | Pride
Ap-ms Tcr Primary cd4+ and cd8+ human t cell Crispr/cas9 Interactome Ost tag 
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