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ProteomicsProteomics Using Proteases Alternative to Trypsin Benefits from Sequential Digestion with Trypsin
High specificity and ease of use make trypsin the most used enzyme in proteomics. Proteases with complementary cleavage specificity to trypsin have been applied to obtain additional data. However, use of proteases with broad specificity proved especially challenging. In this work, we analyzed the ch...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2021-06-07 | PXD017321 | Pride
Alternative proteases Sequential digest 
ProteomicsSurfactant and chaotropic agent assisted sequential extraction/on pellet digestion (SCAD) for enhanced proteomics
As a popular sample preparation approach, Filter-aided sample preparation (FASP) has been widely used in proteomic analysis. However, several limitations have been noted, including sample loss during filtration, repetitive centrifugation steps, and the possibility of breakage of filtration membrane....
ORGANISM(S): Homo sapiens (Human) 
2018-10-19 | PXD008967 | Pride
Urea Protein digestion Protein detection Protein extraction Peptide sequencing Lfq Scad Ibaq Fasp 
ProteomicsAn integrated workflow for cross-linking/mass spectrometry
We present a concise workflow to enhance the mass spectrometric detection of cross-linked peptides by introducing sequential digestion and the database search software Xi. We use sequential digestion to reduce the peptide size and increase identification rates. The methodology is independent of samp...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Gallus gallus (Chicken) Chaetomium thermophilum Equus caballus (Horse) 
2019-08-27 | PXD008550 | Pride
Xi Sequential digestion Cross-linking mass spectrometry 
UnknownIncreasing phosphoproteomic coverage through sequential digestion by complementary proteases.
Not available
S-EPMC3592360 | biostudies-literature
ProteomicsTandem LysC/trypsin digestion performs efficient in detergent conditions and under constant denaturating environments
All shotgun proteomics experiments rely on efficient proteolysis steps for sensitive peptide/protein identification and quantification. Continuous protocol improvement is therefore a constant topic in proteomics research. Previous reports suggest that the sequential tandem LysC/trypsin digest yields...
ORGANISM(S): Escherichia coli Bacteria 
2020-04-30 | PXD013273 | Pride
Shotgun proteomics Protein digestion Lysc Trypsin 
ProteomicsSequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry
Cross-linking/mass spectrometry has become an important approach for studying protein structures and protein-protein interactions. The amino acid composition of some protein regions impedes the detection of cross-linked residues, although it would yield invaluable information for protein modelling. ...
ORGANISM(S): Homo sapiens (Human) Schizosaccharomyces pombe 
2019-03-07 | PXD011459 | Pride
Lc-msms Cross-linking Yeast Human taf4-12 complex 
UnknownSequential Digestion with Trypsin and Elastase in Cross-Linking Mass Spectrometry.
Not available
S-EPMC6458965 | biostudies-literature
UnknownImproved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA.
Not available
S-EPMC2577664 | biostudies-literature
UnknownProteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin.
Not available
S-EPMC7377536 | biostudies-literature
UnknownSubtyping botulinum neurotoxins by sequential multiple endoproteases in-gel digestion coupled with mass spectrometry.
Not available
S-EPMC4582766 | biostudies-literature
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