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ProteomicsIdentification of herpes virus proteines palmitoyled in RPE-1 cells
Protein palmitoylation regulates diverse aspects of cellular function and organization and plays a key role in host immune responses to infection. Palmitoylation also modulates the function and localization of virus-encoded proteins. In Serwa et al. (doi:10.1016/j.chembiol.2015.06.024), we employed ...
ORGANISM(S): Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) Human herpesvirus 2 (strain 186) (HHV-2) (Human herpes simplex virus 2) Human herpesvirus 1 (strain KOS) (HHV-1) (Human herpes simplex virus 1) 
2022-03-02 | PXD002708 | Pride
Palmitoylation Chemical proteomics Tagging by substrate Acyl-rac Herpes virus 
ProteomicsCo-translational and Post-translational protein myristoylation in HeLa cells
Protein N-myristoylation is a ubiquitous co- and post-translational modification that has been implicated in the development and progression of a range of human diseases. In E. Thinon, R. A. Serwa, et al., Nature Communications 2014, doi:10.1038/ncomms5919, we report the global N-myristoylated prote...
ORGANISM(S): Homo sapiens (Human) 
2018-07-31 | PXD002687 | Pride
Co-translational modification Post-translational modification Apoptosis Protein myristoylation 
ProteomicsProfiling N-myristoyltransferase activity and inhibition in breast cancer cells (MD-MB-231) using Sortase-A labelling
We report a novel application for Sortase A (SrtA), labeling N-terminal Gly proteins across the proteome at endogenous levels. We combined SrtA labeling of whole-cell lysates with a potent and selective NMT inhibitor, delivering a method capable of quantifying changes in cellular NMT activity in mul...
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD010634 | Pride
Sortase a  srta  n-myristoylation  nmt 
ProteomicsHYPE mediated AMPylation profiling in human lysates
We describe here the first example of global chemoproteomic screening and substrate validation for HYPE mediated AMPylation in mammalian cell lysate. Through quantitative mass spectrometry-based proteomics coupled with novel chemoproteomic tools providing MS/MS evidence of AMP modification we identi...
ORGANISM(S): Homo sapiens (Human) 
2015-12-02 | PXD002601 | Pride
Hype Chemical proteomics Ampylation Fic 
ProteomicsHYPE mediated AMPylation site profiling with YnATP in human lysates
We describe here the first example of global chemoproteomic screening for HYPE mediated AMPylation sites of human proteins. Catalytically active mutant HYPE E234G was applied in combination with an alkynylated ATP and an alkynyl-biotinylated side ID reagent for capture of chemoenzymatically tagged ...
ORGANISM(S): Homo sapiens (Human) 
2015-12-02 | PXD003053 | Pride
Human Protein ampylation Chemoproteomics 
ProteomicsChanges in Caenorhabditis elegans proteome after dnj-21 knockdown
Dysfunction of the motor subunit of the TIM23 translocase, the PAM complex located on the matrix side of the mitochondrial inner membrane in Saccharomyces cerevisiae, was shown to cause a decrease in mitochondrial protein import and precursor accumulation in the cytosol. We used an analogous model t...
ORGANISM(S): Homo sapiens (Human) Caenorhabditis elegans 
2021-05-19 | PXD023830 | Pride
Caenorhabditis elegans Dnj-21 knockdown Mitochondria Mitochondrial stress 
ProteomicsIdentification of binding partners of the purified TOM complex from HEK293T cells
To investigate the interactome of the human translocase of the outer mitochondrial membrane (TOM) complex, we performed immunoaffinity purification of TOMM22-FLAG from HEK293T cells followed by LC-MS/MS analysis. This approach allowed us to identify proteins that co-purify with the TOM complex under...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD064520 | Pride
Tom complex Tomm22-flag ip Hek293t 
ProteomicsProteomic analysis of FKBP8 knockdown in HEK293T cells
To investigate the effects of FKBP8 depletion in HEK293T cells, cells were transfected with siRNA targeting FKBP8 or with a non-targeting control siRNA for 48 hours. After transfection, cells were collected; 10% of the material was used for whole-cell proteome analysis, and the remaining 90% was use...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD070730 | Pride
Fk506-binding protein Knockdown Fkbp8 Mitochondria Hek293t 
ProteomicsShotgun analysis of proteins co-immunoprecipitating with OCIAD1-FLAG
Interactome of OCIAD1-FLAG was studied. TMT-based relative quantification of protein levels was performed in eluates from mitochondria of HEK293 cells that expressed OCIAD1-FLAG vs empty vector and from mitochondria of OCIAD1-KO cells that expressed OCIAD1-FLAG vs empty vector.
ORGANISM(S): Homo sapiens (Human) 
2024-11-08 | PXD043863 | Pride
Coip Ociad1 Mitochondria Hek293 
ProteomicsHuman OCIAD2 binding partners and the effect of OCIAD2 depletion on mitochondrial protein levels
Assembly of the oxidative phoshorylation dimeric complex III (CIII2) in the mitochondrial inner membrane is an intricate process in which many factors are involved. Despite many studies this process is yet to be completely understood. Here we report the identification of human OCIAD2 (Ovarian Carcin...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD023025 | Pride
Oxidative phosphorylation Mitochondria Ociad2 Oxphos complex iii 
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