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ProteomicsInterval-based secretomics unravels the time-dependent acute phase response in hepatocyte model systems
Mass spectrometry based secretomics approaches preferentially utilize serum-free culture conditions to circumvent serum induced interference and enable sufficient analytical depth. However, this negatively affects a wide range of cellular functions and thus reduces the biological relevance. Moreover...
ORGANISM(S): Homo sapiens (Human) 
2022-05-04 | PXD027821 | Pride
Il1b Heparg Shedding Secretomics Liver Acute-phase response Interval-based secretomics Il6 Hepg2 
ProteomicsQuantitative secretomics during cardiomyogenic differentiation of human pluripotent stem cells
Cardiovascular diseases are a major cause of life-threatening burden around the world. The heart has a very low regeneration capacity and donor organs for transplantation are scarce. Therefore regeneration of lost myocardium with stem cell-derived cardiomyocytes (CMs) provides an attractive strategy...
ORGANISM(S): Homo sapiens (Human) 
2018-06-20 | PXD008554 | Pride
Hescs Cardiomyogenesis Secretomics Hipscs Lc-ms/ms 
ProteomicsParadoxical drug sensitization of lung cancer cells by stromal fibroblast-secreted IGF-binding proteins: Secretomics
Cancer-associated fibroblasts (CAFs) are an integral part of the tumor microenvironment often linked to drug resistance. Here, we report that CAFs, but not normal fibroblasts, can promote either resistance or unexpected drug sensitization of different lung cancer cells. Using unbiased secretomics, t...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD023626 | Pride
Lung cancer Secretomics Fibroblasts Targeted therapy 
ProteomicsSecretomics of Jurkat cells treated with sanglifehrin A or cyclosporin A
Proteins secreted from Jurkat cells upon treatment with sanglifehrin A or cyclosporin A were profiled.
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD031010 | Pride
Secretomics Natural products 
ProteomicsBenchmarking of different methods for obtaining high quality secretomics data
The main drawback of secretome analyses are contaminating proteins derived from culture medium or proteins released from dying cells which are hard to distinguish from proteins actively secreted by cells. Therefore, several approaches have been applied allowing to remove contamination from secretome...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD018895 | Pride
Supernatant Secretomics Secreteome Benchmarking Conditioned medium 
ProteomicsHuman Beta Defensin-2-mediated protection of human skin barrier in vitro; a mechanistic evaluation
I this project we performed global proteomic and secretomic analyses, following HBD2 stimulation, with or without V8-mediated damage, demonstrating minimal impact of the peptide alone on undamaged cells and showing that HBD2 did not induce expression of known antiproteases, but rather may function b...
ORGANISM(S): Homo sapiens (Human) 
2023-02-09 | PXD037844 | Pride
Defensins  antimicrobial host defence peptide  atopic dermatitis  staphylococcus aureus 
ProteomicsSecretomics reveals gelatinase substrates at the blood-brain barrier that are implicated in astroglial barrier function
The[Q3] gelatinases, matrix metalloproteinase 2 (MMP-2) and MMP-9, are key for leukocyte penetration of the brain parenchymal border in neuroinflammation and the functional integrity of this barrier; however, it is unclear which MMP substrates are involved. Using a tailored, sensitive, label-free ma...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-24 | PXD043521 | Pride
Gelatinase Secretomics Mass spec 
ProteomicsMulti-omics of colon tumor progression organoids reveals putative mediators of colorectal cancer metastasis
The development of metastasis severely reduces the life expectancy of patients with colorectal cancer (CRC). Loss of SMAD4 is a key event in late-stage CRC resulting in the progression to metastatic CRC in 10-30% of the cases. However, the biological processes and underlying molecular mechanisms tha...
ORGANISM(S): Homo sapiens (Human) 
2022-12-11 | PXD036441 | Pride
Whole-cell proteomics Secretomics Colon cancer Smad4 Metastasis 
ProteomicsSecretomics of TGF-B1-stimulated IMR-90 cells with and without sanglifehrin A
Proteins secreted from TGF-B1-stimulated IMR-90 cells with and without sanglifehrin A were profiled.
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD029541 | Pride
Natural product Fibrosis Secretome 
ProteomicsSecretomics of sponge whole-body collapse
Sponges (Porifera) are early-branching Metazoa who do not posess muscles or neurons, however are able to undergo a whole-body movement that involves the closure of their canal system and collapse of an epithelial tent. In this study we profile proteomic responses of the freshwater sponge Spongilla l...
ORGANISM(S): Spongilla lacustris 
2024-01-04 | PXD044175 | Pride
Inflammation Evolution Spongilla Secretomics 
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