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ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
ProteomicsRNase L activating 2′-5′ oligoadenlyates bind ABCF1, -3 and Decr-1
Identification of 2′–5′ OA binding proteins in human (HeLa), mouse (BMDM) and fly (Schneider S2) cells using affinity purification mass spectrometry (AP-MS). Pulsed SILAC-based translational profiling in HeLa and HeLa S3 cells treated with 2′–5′ OA or OH-2′–5′ OA.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Drosophila melanogaster (Fruit fly) 
2023-08-18 | PXD030134 | Pride
Psilac Ap-ms Lc-ms/ms 
ProteomicsHuman mitochondrial TACO1-FLAG SILAC AP-MS
The prokaryotic translation elongation factor P (EF-P), and the eukaryotic/archeal counterparts eIF5A/aIF5A, are proteins that serve a crucial role in mitigating ribosomal stalling during the translation of specific sequences, notably those containing consecutive proline residues. Although mitochond...
ORGANISM(S): Homo sapiens (Human) 
2024-09-06 | PXD051090 | Pride
Ap-ms Mitochondria Taco1 Silac 
ProteomicsAP-MS Screening for novel Ribosome Biogenesis factors revealing 55LCC
Data from our initital screening for novel biogenesis factors in human ribosome biogenesis, revealing 55LCC interaction and interaction within 55LCC. Further, the dataset includes 55LCC depletion data trapping the pre-60S, enriching non-released biogenesis factors on the pre-60S. In addition transge...
ORGANISM(S): Homo sapiens (Human) 
2026-07-29 | PXD065027 | Pride
Human Ap-ms Ribosome 55lcc 
ProteomicsPDGFR signaling in recessive dystrophic epidermolysis bullosa
Seven sets of SILAC-based AP-MS experiments were performed analyzing phosphotyrosine signaling after 0, 5, and 10 min of PDGF-BB treatment of primary human skin fibroblasts. For this, proteins phosphorylated on tyrosine residues were enriched using the 4G10 antibody.
ORGANISM(S): Homo sapiens (Human) 
2021-11-01 | PXD022985 | Pride
Skin Fibroblast Phosphotyrosine Human Dystrophic epidermolysis bullosa 
ProteomicsEstablishment of functional protein interaction networks of so far undescribed human mitochondrial proteins by q-AP-MS
The overall aim of the project is the definition and characterization of the mitochondrial proteome of human cells, referred to as MitoCoP. To this end, we combined complementary strengths of subtractive proteomics, ImportOmics and subcellular protein profiling in a global multidimensional classific...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD018182 | Pride
Proteomics Affinity purification-mass spectrometry Silac Mitochondria 
ProteomicsAP-MS Screening for novel Ribosome Biogenesis factors revealing 55LCC
Data from our initital screening for novel biogenesis factors in human ribosome biogenesis, revealing 55LCC interaction and interaction within 55LCC. Further, the dataset includes 55LCC depletion data trapping the pre-60S, enriching non-released biogenesis factors on the pre-60S. In addition transge...
ORGANISM(S): Homo sapiens (Human) 
2026-07-29 | PXD065526 | Pride
Human Ap-ms Ribosome Spata5l1 55lcc Spata5 Cinp. c1orf109 
ProteomicsAnalysis of the nascent proteome during murine norovirus infection
SILAC- and AHA-labelling analysis of Raw264.7 cells infected with MNV-1 at MOI=10TCID50/cell and harvested at 6.5 and 10.5 hours post infection. AP-MS enrichment of AHA-labelled nascent polypeptides represents the first quantitative neoproteomics analysis of norovirus infected cells.
ORGANISM(S): Mus musculus (Mouse) 
2021-07-02 | PXD025169 | Pride
Raw264.7 Aha Mnv Silac 
ProteomicsAP-MS analysis of murine and human norovirus VPg interactomes
The norovirus VPg protein is covalently linked to the viral genome in place of a 5' cap, and functions as a cap-substitute, capable of interacting with translation initiation factors. Following on from our previous study (Chung et. al. 2014, J. BIol. Chem.) we wished to determine the interactome of ...
ORGANISM(S): Homo sapiens (Human) 
2019-09-09 | PXD007585 | Pride
Norovirus Silac Hek293t Vpg Translation Calicivirus 
ProteomicsA high-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC
Epitope tagging and magnetic bead enrichment were combined with SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite Trypanosoma brucei.
ORGANISM(S): Trypanosoma brucei 
2014-05-13 | PXD000663 | Pride
Trypanosoma brucei Orbitrap velos Ap-ms 
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