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ProteomicsLYSET/TMEM251 SILAC Co-IP from MIA PaCA-2
Endogenous LYSET/TMEM251 was isolated via Co-IP from MIA PaCa-2 cells. LYSET KO cells were used as controls. Cells were grown in SILAC medium for 3 passages
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD035821 | Pride
Silac co-ip Human Lyset Tmem251 
ProteomicsCo-IP of v-ATPase subcomplexes from MEFs under amino acid starvation and restimulation
v-ATPase Vo and V1 domains were isolated via Co-IP from MEFs expressing HA-Atp6V1B2 and FLAG-Atp6VoaA3 using HA- and FLAG-tag antibodies. Treatment groups were full medium (control), amino acid starvation (60 min) and restimulation (30 min). Cells were grown in SILAC medium for 3 passages.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-20 | PXD030174 | Pride
Mefs Co-ip Silac Vatpase Amino acid starvation 
ProteomicsInvestigating the Interactomic Landscape of Survival Motor Neurons (SMN) and the SMN Δ7 truncated protein
This protocol describes a methodology combining TurboID - a recently developed proximity biotinylation technique - with conventional epitope-tag based co-immunoprecipitation (Co-IP) to analyse protein-protein interactions (PPIs) in cell culture systems. This integrated approach allows for the target...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD066858 | Pride
Proximity biotinylation Turboid Interactomics Silac Co-immunoprecipitation Proteomics Spinal muscular atrophy 
ProteomicsSILAC immunoprecipitations for rabbit haemorrhagic disease virus (RHDV) proteins p23 and RNA-dependent RNA polymerase
Rabbit haemorrhagic disease virus (RHDV) belongs to the family Caliciviridae, genus Lagovirus and is used in Australia as a biocontrol tool to keep the population of european rabbits low. This virus has a positive-sense single-stranded RNA genome that encodes structural (capsid) and non-structural p...
ORGANISM(S): Oryctolagus cuniculus cuniculus 
2022-10-14 | PXD031619 | Pride
Interactors Silac Co-ip Rhdv 
ProteomicsTriple SILAC identified progesterone-independent and dependent PRA and PRB interacting partners in human breast cancer cells
Progesterone receptor (PR) isoforms, PRA and PRB, both progesterone-independent and dependent modulated the biology of breast cancer cells. The different phenotypes in breast cancer mediated by PRA and PRB could due to the differences of their structures, leading to the distinct protein interacting ...
ORGANISM(S): Homo sapiens (Human) 
2021-03-08 | PXD023920 | Pride
Progesterone receptor Silac Breast cancer Interacting partners 
ProteomicsSILAC-based quantitative Nox4-Co-immunoprecipitation
Within the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Interacting proteins were screened ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080772 | MassIVE
ProteomicsSILAC-based quantitative Nox4-Co-immunoprecipitation
Within the family of NADPH oxidases, Nox4 is unique as it is predominantly localized in the endoplasmic reticulum, has constitutive activity and generates H2O2. We hypothesize that these features are consequences of a so far unidentified Nox4-interacting protein. Interacting proteins were screened ...
ORGANISM(S): Homo sapiens (Human) 
2016-02-17 | PXD003514 | Pride
Lc-msms Macromolecular complexes Silac Co-immunoprecipitation 
ProteomicsCell-specific analysis of tumour-endothelial contact-intiated signalling - PROTEOMICS
Metastasizing tumor cells exit the vascular system through dynamic interactions with endothelial cells that line the internal surface of vessels. While extravasation is a key event within the metastatic cascade, the signals regulating tumor cell adhesion to the endothelium and their subsequent trans...
ORGANISM(S): Homo sapiens (Human) 
2016-02-12 | PXD003048 | Pride
Epha2 Mda-mb-231-lm2 "contact-initiated signaling" Huvec 
ProteomicsDynamic profiling of the protein life cycle in response to pathogens (protein)
Protein expression is regulated by production and degradation of mRNAs and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics to build a quantitative genomic model of the differential regulation of gene expressio...
ORGANISM(S): Mus musculus 
2015-03-06 | E-GEOD-59792 | biostudies-arrayexpress
ProteomicsAnalysis of FAM65A interactions
We identified FAM65A as a novel interactor of RHOA. To reveal other binding partners of FAM65A, we carried out a quantitative IP-MS, using SILAC labelled HeLa cells ectopically expressing GFP-FAM65A or GFP alone as control. Experiments were performed in duplicate with switched labelling and specific...
ORGANISM(S): Homo sapiens (Human) 
2016-11-01 | PXD004933 | Pride
Ip-ms  silac  fam65a  rho 
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