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ProteomicsLYSET/TMEM251 SILAC Co-IP from MIA PaCA-2
Endogenous LYSET/TMEM251 was isolated via Co-IP from MIA PaCa-2 cells. LYSET KO cells were used as controls. Cells were grown in SILAC medium for 3 passages
ORGANISM(S): Homo sapiens (Human) 
2022-09-29 | PXD035821 | Pride
Silac co-ip Human Lyset Tmem251 
ProteomicsTET1 interactome by SILAC IP-MS in mouse ESCs
TET1 maintains hypomethylation at bivalent promoters through its catalytic activity in embryonic stem cells (ESCs). However, whether and how TET1 exerts catalytic activity-independent functions in regulating bivalent genes is not well understood. Therefore, we mapped the TET1 interactome in mouse ES...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-10 | PXD033587 | Pride
Silac Ip-ms Tet1 Escs 
ProteomicsSILAC IP-Mass spectrometry of TTLL-transfected HEK cells
Identification of the non-tubulin substrates of tubulin glycylases, TTLL3/TTLL8 and tubulin glutamylases TTLL4/TTLL5 using SILAC-IP-MS/MS quantitative mass spectrometry approach. HEK cells transfected with the respective enzymes were lysed and the proteins modified by the enzymes enriched by immunop...
ORGANISM(S): Homo sapiens (Human) 
2026-05-18 | PXD065251 | Pride
Hek cells Ttlls Ip-ms/ms Silac 
ProteomicsCo-IP of v-ATPase subcomplexes from MEFs under amino acid starvation and restimulation
v-ATPase Vo and V1 domains were isolated via Co-IP from MEFs expressing HA-Atp6V1B2 and FLAG-Atp6VoaA3 using HA- and FLAG-tag antibodies. Treatment groups were full medium (control), amino acid starvation (60 min) and restimulation (30 min). Cells were grown in SILAC medium for 3 passages.
ORGANISM(S): Mus musculus (Mouse) 
2022-08-20 | PXD030174 | Pride
Mefs Co-ip Silac Vatpase Amino acid starvation 
ProteomicsSILAC APMS analysis of CCDC103 interactome
To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and c...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD009498 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of DNAAF2 interactome
To get insights into the function of DNAAF2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP- DNAA...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009501 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of Pih1D3 interactome
To get insights into the function of Pih1D3 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009499 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsSILAC APMS analysis of Pih1D2 interactome
To get insights into the function of Pih1D2 protein, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-Pih1D...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009520 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
ProteomicsIdentification of SMIM26 interacting proteins
The SILAC IP proteins were digested by FAST and peptides were used to analysis.
ORGANISM(S): Homo sapiens (Human) 
2023-03-29 | PXD039926 | Pride
Silac ip 
ProteomicsSILAC APMS analysis of RPAP3 C-term interactome
To get insights into the function of RPAP3, we characterized its partners by performing a proteomic analysis in human cells. We fused the Cterm part of RPAP3 domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of...
ORGANISM(S): Homo sapiens (Human) 
2018-06-10 | PXD009518 | Pride
Human Ltq-orbitrap Hela cells Apms Silac 
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