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ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
ProteomicsComparative proteomic profiling of 3T3-L1 adipocyte differentiation using SILAC quantification
Comparative proteomic profiling of 3T3-L1 adipocyte differentiation using SILAC quantification
ORGANISM(S): Mus musculus (Mouse) 
2020-10-26 | PXD019716 | Pride
Differentiation 3t3-l1 Adipocyte Silac 
ProteomicsBenchmarking LFQ, SILAC and MS2/MS3-based TMT quantification strategies for large-scale phosphoproteomics
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-03-13 | PXD007145 | Pride
Phosphoproteomics Ms3 Ms2 Tmt Silac Lfq Technical Benchmark Quantification 
ProteomicsMaximally-multiplexed proteome quantification platform for isotopic metabolic and chemical labeling
Quantitative proteomic platforms based on precursor intensity in mass spectrometry (MS1-level) uniquely support in vivo metabolic labeling with superior quantification accuracy but suffers from limited multiplexity (≤3-plex) and frequent missing quantities. Herein, we present a maximally-multiplexed...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD009952 | Pride
Diethylation Heat shock Silac-6plex Protein quantification Hela cell Quantification Silac De-6plex Heat shock response 
ProteomicsPROMIS-Quan: a novel proteomic method for plasma biomarker quantification
Unbiased proteomic analysis of plasma samples holds the promise to reveal clinically invaluable disease biomarkers. However the tremendous dynamic range of the plasma proteome has so far hampered the identification of such low abundant markers. To overcome this challenge we analyzed the plasma micro...
ORGANISM(S): Homo sapiens (Human) 
2015-01-28 | PXD001194 | Pride
Plasma Microparticles Super-silac Biomarkers 
ProteomicsCombining data independent acquisition with spike-in SILAC (DIA-SiS) improves proteome coverage and quantification )
Data Independent Acquisition (DIA) is increasingly preferred over Data Dependent Acquisition (DDA) due to its higher throughput and fewer missing values. Whereas DDA often utilizes stable isotope labeling to improve quantification, DIA mostly relies on label-free approaches. Efforts to integrate DIA...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-10-14 | PXD052080 | Pride
Multiplexing Dia Plexdia Silac Proteomics Benchmark 
ProteomicsCoisolation of peptide pairs for peptide identification and MS/MS-based quantification
SILAC-based metabolic labeling is a widely adopted proteomics approach that enables quantitative comparisons among a variety of experimental conditions. Despite its quantitative capacity, SILAC experiments analyzed with data dependent acquisition (DDA) do not fully leverage peptide pair information ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-11-03 | PXD033016 | Pride
Ms/ms Yeast Silac Peptide pairs Quantification Comet 
UnknownProteomics of microparticles with SILAC Quantification (PROMIS-Quan): a novel proteomic method for plasma biomarker quantification.
Not available
S-EPMC4390257 | biostudies-literature
ProteomicsmTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Silac Tmt Isobaric Sh-sy5y Svgp12 Metabolic 
UnknownImproved SILAC Quantification with Data-Independent Acquisition to Investigate Bortezomib-Induced Protein Degradation.
Not available
S-EPMC8256668 | biostudies-literature
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