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GenomicsmiR-146a Pulldown-sequencing data
miR-146a Pulldown-sequencing data
PRJNA675568 | ENA
GenomicsmiR-150 Pulldown-sequencing data
miR-150 Pulldown-sequencing data
PRJNA675569 | ENA
ProteomicsBLM-G4 pulldown IP-MS in human colon cancer HT-29 cells
the experiment uses biotin-labelled BLM-G4 to incubate with HT-29 cell lysates, and uses streptavidin agarose beads to pulldown BLM-G4-binding proteins. Utilize LC-MS/MS to annotate and quantify the proteins pulled down by BLM-G4.
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075345 | Pride
Pulldown Blm-g4 Ip-ms Ht-29 cell 
Proteomicsbiotin RNA pulldown assay of lnc-GD2H and mass spectrometry
Vectors carrying lnc-GD2H and antisense lnc-GD2H were linearized with the corresponding restriction enzymes to prepare template DNAs for in vitro transcription. Biotinylated RNAs were mixed with proteins extracted from C2C12 cells, followed by targeting of the RNAs with streptavidin beads. After SDS...
ORGANISM(S): Mus musculus (Mouse) 
2021-05-18 | PXD025292 | Pride
C2c12 Myoblast Biotin rna pulldown Ms Mice Lnc-gd2h 
ProteomicsPulldown of P180/RRBP1 interacting protein
P180/RRBP1 is an integral ER-resident protein known to be involved in mRNA binding and translation at the ER. We found that P180/RBBP1 is enriched in neuronal axons. To acquire more knowledge about its interactome and confirmation that P180/RRBP1 is interacting with ribosomes, we performed an unbias...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2024-10-14 | PXD050948 | Pride
Brain P180/rrbp1 Hek293 
ProteomicsRNA pulldown-MS, Dlg4 RNA in mouse brain
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034159 | Pride
Pulldown M6a Rna Lc-ms/ms 
ProteomicsRNA pulldown-MS, Dlg4 RNA in mHippoE-2 cells
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034161 | Pride
Pulldown M6a Rna Lc-ms/ms 
ProteomicsStreptavidin biotin-CCW16 pulldown to determine CCW16-modified proteins in HeLa lysate
The SUMO-targeted E3 ubiquitin ligase RNF4 plays an important role in safeguarding genome and proteome integrity. RNF4 recognizes polySUMO modified proteins and induces their proteolytic or non-proteolytic ubiquitylation. Based on the key functions of RNF4 maintaining proteome and genome stability, ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-21 | PXD056954 | Pride
Ccw16 Targets Hela Lfq Rnf4 
GenomicsRNA-seq of biotin-labelled-miR190b pulldown
RNA-seq of biotin-labelled-miR190b pulldown
PRJNA816541 | ENA
TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
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