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ProteomicsProteomic analysis of subchloroplastic compartments
Arabidopsis thaliana chlorosplasts extraction followed by subchloroplastic compartments fractionation (envelope, stroma, thylakoids). Afer SDS-PAGE separation and trypsin digestion, analysis of all gel bands twice by LC-MS/MS on a LTQ-FT
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-23 | PXD000136 | Pride
Sub compartments fractionation Chloroplast Ltq-ft Lc-ms/ms 
ProteomicsParkin-dependent mitophagy occurs via proteasome-dependent stepwise inwardly directed mitochondrial sub-compartment targeting to lysosomes
PINK1/parkin-dependent mitophagy initially involves (phospho) ubiquitin-proteasome-dependent degradation of certain outer mitochondrial membrane (OMM) proteins (e.g. mitofusins) and then the recruitment of autophagy effectors to more stable ubiquitinated OMM proteins. It is widely assumed that such ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD034136 | Pride
Mitochondrial outer membrane  mitophagy  parkin  proteasome  ubiquitin 
ProteomicsCross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topology
The specific functions of cellular organelles and sub-compartments depend on their protein content, which can be characterized by spatial proteomics approaches. However, many spatial proteomics methods are limited in their ability to resolve organellar sub-compartments, profile multiple sub-compartm...
ORGANISM(S): Homo sapiens (Human) 
2024-02-21 | PXD046382 | Pride
Synaptic vesicles Xl-ms Mitochondria Spatial proteomics Dsbso 
ProteomicsEnrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary (CHO) cells have been widely employed for production of recombinant proteins (RP) for research and therapeutic purposes. Indeed, this expression system was used for production of most of approved antibodies (84%) in 2015-2018 period. The success of this cell line for RP product...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-08-27 | PXD019778 | Pride
Organelles Subcellular fractionation Subcellular proteomics Chinese hamster ovary cells 
UnknownDissecting the subcellular compartmentation of proteins and metabolites in arabidopsis leaves using non-aqueous fractionation.
Not available
S-EPMC4159647 | biostudies-literature
UnknownTargeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1.
Not available
S-EPMC3566231 | biostudies-literature
UnknownCorrelation profiling of brain sub-cellular proteomes reveals co-assembly of synaptic proteins and subcellular distribution.
Not available
S-EPMC5608747 | biostudies-literature
ProteomicsArabidopsis thaliana non aqueous fractionation LC-MS/MS analysis of genotypes Ler (Landsberg erecta) and gin2-1 (glucose insensitive (hexokinase 1)) time series analysis under normal, chilling temperature and highlight+heat.
Plant cells are characterized by a high degree of compartmentalization and a diverse proteome and metabolome. Only a very limited number of studies has addressed combined subcellular proteomics and metabolomics which strongly limits biochemical and physiological interpretation of large-scale omics d...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-08-05 | PXD013646 | Pride
Arabidopsis thaliana Time series Subcellular protein marker Subcellular analysis Hexokinase 1 Cold Highlight Non aqueous fractionation Photorespiration Gin2-1 
ProteomicsTowards a quantitative assessment of the integral magnetosome membrane sub-proteome
Magnetosomes are membranous organelles than contain crystals of magnetic minerals and enable magnetotactic bacteria to orientate in magnetic fields. The enveloping magnetosome membrane was found to contain a subset of specific proteins that are encoded within a genomic island. However, along with pr...
ORGANISM(S): Magnetospirillum gryphiswaldense 
2017-10-24 | PXD006166 | Pride
Organelle Membrane proteome Magnetosome 
UnknownSubcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism.
Not available
S-EPMC6812369 | biostudies-literature
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