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ProteomicsPrevention of mitochondrial proteotoxicity by formation of a chaperone-dependent protective shell around aggregated polypeptides
The potential proteotoxicity of mitochondrial aggregates in yeast cells is reduced by a sequestration of affected polypeptides into a mitochondrial protein quality control compartment (IMiQ). Based on the expression of an aggregation-prone protein in the mitochondrial matrix, we determined the effec...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-08-21 | PXD059828 | Pride
Mitochondria Hsp70 Chaperone Protein aggregation Proteostasis Yeast 
ProteomicsProteomic analysis of the role of the quality control protease LONP1 in mitochondrial protein aggregation
The mitochondrial matrix protease LONP1 is an essential part of the organellar protein quality control system. LONP1 has been shown to be involved in respiration control and apoptosis. Furthermore, a reduction in LONP1 level correlates with ageing processes. Up to now, the effects of a LONP1 defect ...
ORGANISM(S): Homo sapiens (Human) 
2022-01-31 | PXD026941 | Pride
Human Protein aggregation Heat stress Lonp1 atp-dependent protease Mitochondria Genetic alteration via crispr/cas Protein degradation 
ProteomicsInsights into the mechanism of human deiodinase 1
The three isoenzymes of iodothyronine deiodinases (DIO1-3) are membrane-anchored homo-dimeric selenoproteins which share the thioredoxin fold structure. Several questions regarding their catalytic mechanisms still remain open. Here, we addressed the roles of several cysteines which are conserved amo...
ORGANISM(S): Spodoptera frugiperda Homo sapiens (Human) 
2022-05-31 | PXD032784 | Pride
Lc-msms Selenoprotein  selenocysteine  thyroid hormone  kinetic analysis 
ProteomicsProteomic characterization of Hsp78-interacting proteins highlights chaperone role during protein aggregation in mitochondria under heat stress - Part A Protein Interactions
Chaperones of the Hsp100 chaperone family support protein homeostasis, the maintenance of protein activity under stress, by refolding aggregated proteins or targeting them for degradation. Hsp78, the ClpB-type mitochondrial member of the Hsp100 family, can be found in lower eukaryotes like yeast. Al...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-09-20 | PXD030405 | Pride
Yeast  cell biology  mitochondria  heat stress  protein aggregation  proteostasis  chaperone  hsp78 
ProteomicsProteomic characterization of Hsp78-interacting proteins highlights chaperone role during protein aggregation in mitochondria under heat stress - Part B Aggregation
Chaperones of the Hsp100 chaperone family support protein homeostasis, the maintenance of protein activity under stress, by refolding aggregated proteins or targeting them for degradation. Hsp78, the ClpB-type mitochondrial member of the Hsp100 family, can be found in lower eukaryotes like yeast. Al...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-09-20 | PXD030436 | Pride
Hsp78 Yeast Cell biology Mitochondria Chaperone Protein aggregation Proteostasis Heat stress 
ProteomicsPFN4 is required for acrosome biogenesis by regulating autophagy through PI3K/AKT/mTOR pathway
We generated Pfn4 knockout mice and showed that male mice are infertile and acrosome biogenesis is impaired from first phase of Development (Golgi-phase). Autophagy participates in acrosome formation and LC-MS data showed proteins related to autophagy, PI3K/AKT signaling and mTOR are enriched in PFN...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-15 | PXD030394 | Pride
Infertility Autophagy Lc-ms Mouse Profilin 4 
ProteomicsDisease-linked lysine glutarylation impairs function and interactions of mitochondrial proteins and contribute to mitochondrial heterogeneity
Inherited deficiencies of the lysine and tryptophan catabolic pathways, due to mutations in the glutaryl-CoA-dehydrogenase (GCDH) gene, cause glutaric aciduria type 1 (GA1). In mammals two metabolic routes for L-lysine oxidation exist, the mitochondrial saccharopine pathway, which is predominant in ...
ORGANISM(S): Mus musculus (Mouse) 
2018-07-11 | PXD007881 | Pride
Metabolism Molecular biology of disease Protein interaction Glutaryl-coa-dehydrogenase Glutaryl-proteome Proteolysis & proteomics Post-translational modifications Glutamate dehydrogenase 
ProteomicsTMT-based quantitative proteomics of exosomes from porcine colostrum and milk
Colostrum and milk have high nutritional value and provide a complete diet for neonates, along with bioactive substances which modulate various functions such as immune defense. Exosomes are membranous vesicles of endocytic origin, recently been considered as major players in cell-cell communication...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2022-02-08 | PXD025515 | Pride
Exosomes Tmt labelling Extracellular vesicles Colostrum Milk Orcine Pig Lc-ms/ms 
ProteomicsDifferential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants
The proteome of two sperm sources, epididymal and ejaculated sperm, was compared and associated with sperm freezing capacity using samples collected from three wild small ruminants species. Protein identification was performed using two databases separately (Ovis aries and Capra hircus NCBI) and res...
ORGANISM(S): Ovis aries musimon Capra pyrenaica Rupicapra pyrenaica 
2021-05-04 | PXD017462 | Pride
Germplasm bank Proteomics Tmt Mass spectrometry Sperm Goat Sheep Cross-species Cryopreservation 
ProteomicsIdentification of a Ste20-like kinase-dependent network orchestrating cytoskeletal and synaptic development of cortical neurons. Untargeted phosphorylation analysis
The Ste20-like kinase (Slk) is a serine/threonine kinase that regulates cytoskeletal organization, apoptosis, and cell polarity in non-neuronal cells. In neurons, Slk guides dendrite morphogenesis and inhibitory synapse stability, controlling excitation-inhibition balance through parvalbumin interne...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2026-10-02 | PXD073661 | Pride
Germinal center kinases Kinase signaling Affinity purification-mass spectrometry Lc/ms Protein-protein interactions Phosphoproteomics 
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