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MetabolomicsThermal Profiling Maps Multilayered Organization of the Mitochondrial Proteome

Mitochondria execute essential metabolic, biosynthetic, and signaling functions, yet the regulatory principles governing their proteome remain incompletely understood. Here, we develop a thermal proteome profiling (TPP) workflow applied directly to purified mitochondria, enabling systematic analy...

2025-12-08 | MTBLS7386 | MetaboLights
ProteomicsOff-target profiling of Olaparib using Thermal Proteome Profiling
Explore off-targets of Olaparib using 2D Thermal Proteome Profiling (2D-TPP)
ORGANISM(S): Homo sapiens (Human) 
2024-05-13 | PXD046614 | Pride
Thermal proteome profiling Parp 2d-tpp Olaparib 
ProteomicsOff-target profiling of Niraparib and Olaparib using Thermal Proteome Profiling
Explore off-targets of Niraparib and Olaparib using 2D Thermal Proteome Profiling (2D-TPP)
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD044334 | Pride
Thermal proteome profiling Parp Niraparib 2d-tpp Olaparib 
ProteomicsMembrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamic interactions
Integral membrane proteins (IMPs) are key targets for small-molecule therapeutics. However, robust, unbiased, and detergent-free methods to probe on- and off-target interactions within this protein class remain underdeveloped. Previously, we introduced the Peptidisc membrane mimetic (MM) for water-s...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-17 | PXD068828 | Pride
Membrane proteins Orbitrap exploris Mus musculus Membrane mimetic thermal proteome profiling Proteomics 
ProteomicsThermal proteome profiling of phosphorylated proteoforms
We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD015993 | Pride
Phosphopeptides Meltome Phosphoproteomics Thermal proteome profiling 
ProteomicsThermal proteome profiling in Escherichia coli
We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-07-09 | PXD009495 | Pride
Target engagement Escherichia coli Drugs Growth phase Meltome Thermal proteome profiling 
ProteomicsThermal Proteome Profiling Datasets for smarTPCA
Chemical biology and the application of small molecules has proven to be a potent perturbation strategy especially for the functional elucidation of proteins, their networks and regulators. In recent years, the cellular thermal shift assay (CETSA) and its proteome-wide extension, thermal proteome pr...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD032942 | Pride
Kinase inhibitors Small molecule arranged thermal proximity coaggregation (smartpca) Tpp with temperature range (tpp-tr) or compound concentration range (tpp-ccr) Hl-60 cell line Thermal proteome profiling (tpp) 
ProteomicsThermal proteome profiling of Escherichia coli mutants
We performed two dimensional thermal proteome profiling (2D-TPP) in Escherichia coli mutants to measure changes in abundance and thermal stability.
ORGANISM(S): Escherichia coli 
2020-09-11 | PXD016589 | Pride
Escherichia coli Genetic perturbation Mutants Thermal proteome profiling 
ProteomicsMembrane mimetic thermal proteome profiling (MM-TPP) towards mapping membrane protein-ligand dynamics
We combine the membrane mimetic (MM) Peptidisc with thermal proteome profiling (TPP) to screen membrane proteomes in detergent-free environments. Using whole mouse liver, we demonstrate the stabilization of integral membrane proteins (IMPs), specifically ATP-binding cassette transporters, through in...
ORGANISM(S): Mus musculus (Mouse) Escherichia coli 
2025-11-17 | PXD055093 | Pride
Membrane mimetic Mouse liver Thermal proteome profiling 
ProteomicsThermal proteome profiling of Salmonella enterica infection
We performed two dimensional thermal proteome profiling (2D-TPP) of the infection time course of Salmonella enterica.
ORGANISM(S): Salmonella enterica subsp. enterica str. xld 
2024-08-08 | PXD043344 | Pride
Salmonella Thermal proteome profiling Infection 
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