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ProteomicsPartial characterization of the glycoconjugates of Trypanosoma theileri
Here, we have started to characterise the surface molecules of T. theileri at the biochemical level. We have applied the same differential solvent extraction and hydrophobic interaction chromatography protocols, developed by Almeida and colleagues [13,14] to those used to isolate the GPI-mucin and G...
ORGANISM(S): Trypanosoma brucei 
2025-09-29 | PXD029837 | Pride
Trypanosoma theileri Mucin Glycoconjugate Glycoinositol-phospholipids Glycosylphosphatidylinositol Gpi 
ProteomicsProbing endogenous peptide acceptor substrate specificities of TbSTT3A and TbSTT3B
We performed glycoproteomics on endogenous parasite glycoproteins using sequential endoglycosidase-H and peptide-N-glycosidase-F digestions, the latter in the presence of H2[18O]. This allowed us to assess the relative occupancies of native N-glycosylation sites by endoglycosidase-H resistant N-glyc...
ORGANISM(S): Trypanosoma brucei 
2017-09-20 | PXD007267 | Pride
Trypanosoma Glycoproteomics Oligosaccharyltransferase N-linked glycosylation Stt3 Glycoprotein biosynthesis Parasitology 
ProteomicsA non-coding role for trypanosome VSG transcripts in allelic exclusion
Bloodstream-form African trypanosomes display mono-telomeric expression of a Variant Surface Glycoprotein (VSG) gene in an inter-chromosomally bridged transcription and splicing compartment, such that the dominant gene produces 10,000 times more transcript than excluded VSG genes. Antigenic variatio...
ORGANISM(S): Trypanosoma brucei 
2025-03-11 | PXD060524 | Pride
Antigenic variation Trypanosoma brucei. Cncrna Monoallelic 
ProteomicsTbSTT3A and TbSTT3B hetero-oligomeric complex
Directly address the oligomeric states of T. brucei STT3 subunits
ORGANISM(S): Trypanosoma brucei 
2017-09-20 | PXD007236 | Pride
Trypanosoma Glycoproteomics Oligosaccharyltransferase N-linked glycosylation Stt3 Glycoprotein biosynthesis Parasitology 
ProteomicsProteomic identification of the UDP-GlcNAc : PI α1-6 GlcNAc-transferase subunits of the glycosylphosphatidylinositol biosynthetic pathway of Trypanosoma brucei.
The first step of glycosylphosphatidylinositol (GPI) anchor biosynthesis in all eukaryotes is the addition of N-acetylglucosamine (GlcNAc) to phosphatidylinositol (PI) which is catalysed by a UDP-GlcNAc : PI α1-6 GlcNAc-transferase. This enzyme has been shown to be a complex of at least seven subuni...
ORGANISM(S): Trypanosoma brucei 
2021-02-26 | PXD022979 | Pride
Glcnac-transferase Tbarv1 Trypanosoma brucei Glycosylphosphatidylinositol 
ProteomicsPrecision-edited histone tails perturb polycistronic gene expression controls in trypanosomes
Transcription of protein coding genes in trypanosomatids is atypical and almost exclusively polycistronic. In Trypanosoma brucei, approximately 150 polycistrons, and 8000 genes, are constitutively transcribed by RNA polymerase II, while a smaller subset can be transcribed by RNA polymerase I. RNA po...
ORGANISM(S): Trypanosoma brucei 
2025-03-11 | PXD061709 | Pride
Trypanosomes h4 t. brucei polycistronic expression control 
ProteomicsNovel analysis on substrate specificities of TbSTT3A and TbSTT3B by glycoproteomics
reprocessed data from Izquierdo et al, Distinct donor and acceptor specificities of Trypanosoma brucei oligosaccharyltransferases
ORGANISM(S): Trypanosoma brucei 
2017-09-20 | PXD007268 | Pride
Trypanosoma Glycoproteomics Oligosaccharyltransferase N-linked glycosylation Stt3 Glycoprotein biosynthesis Parasitology 
ProteomicsOrigins and consequences of kinetoplast loss in trypanosomes
The kinetoplast is the large mitochondrial genome present in the eponymous Kinetoplastida. African trypanosomes can lose their kinetoplast DNA (kDNA), however, when the nuclear-encoded gamma subunit of the mitochondrial ATP-synthase (g-ATPase) is mutated. These mutations are also associated with mul...
ORGANISM(S): Trypanosoma brucei 
2025-12-13 | PXD071938 | Pride
Trypanosoma brucei Mutagenesis Editing Genome Drug resistance Kinetoplastid 
ProteomicsVSG Exclusion Protein 2 (VEX2) affinity purification combined with LC-MS/MS analysis in Trypanosoma brucei
UPF1-like helicases play roles in telomeric heterochromatin formation and X-chromosome inactivation and we focus here on variant surface glycoprotein (VSG) exclusion-factor-2 (VEX2), a UPF1-related protein in the African trypanosome. This bloodstream parasite evades host immunity by employing monoge...
ORGANISM(S): Trypanosoma brucei 
2023-11-20 | PXD040686 | Pride
Vex2 Trypanosoma brucei Cryomilling Lc-ms/ms 
ProteomicsControl of variant surface glycoprotein expression by CFB2 in African trypanosomes and quantitative proteomic connections to translation and cytokinesis initiation
Variant Surface Glycoproteins (VSG) coat parasitic African trypanosomes and underpin antigenic variation and immune evasion. This VSG is a super-abundant virulence factor that is subject to post-transcriptional gene expression controls mediated via the VSG 3’-untranslated region (3’-UTR). To identif...
ORGANISM(S): Trypanosoma brucei 
2022-02-01 | PXD031351 | Pride
Mrna-binding  pbp1 Trypanosoma brucei  mkt1 Proteomics  rnai. 
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