Sort   by:  
 Page size 
ProteomicsStreamlined TMT labeling workflow for proteomics and metaproteomics
This study established a streamlined TMT labeling workflow that is readily compatible with high throughput proteomics and metaproteomics sample preparation. The method was then evaluated using two different sample types, microbiome and intestinal Caco-2 cell line, with TMT11plex mix reagents.
ORGANISM(S): metagenome Homo sapiens (Human) 
2023-02-07 | PXD034791 | Pride
Proteomics Tmt Microbiome Metaproteomics 
ProteomicsStreamlined TMT labeling workflow for proteomics and metaproteomics
This study established a streamlined TMT labeling workflow that is readily compatible with high throughput proteomics and metaproteomics sample preparation. The method was then evaluated using two different sample types, microbiome and intestinal Caco-2 cell line, with TMT11plex mix reagents.
ORGANISM(S): metagenome Homo sapiens (Human) 
2023-02-07 | PXD034936 | Pride
Proteomics Tmt Microbiome Metaproteomics 
ProteomicsComplex-dependent histone acetyltransferase activity of KAT8 determines its role in transcriptional regulation and cellular homeostasis [TMT11plex whole proteome analysis]
Histone acetylation is associated with open chromatin and transcriptionally active genes. Specifically, acetylation of lysine 16 on histone H4 (H4K16ac) has been shown to prevent the assembly of nucleosomal arrays in vitro. This modification is catalyzed by the MYST-family histone acetyltransferase ...
ORGANISM(S): Homo sapiens (Human) 
2021-06-29 | PXD023865 | Pride
Nsl Msl Histone Kat8 
ProteomicsSelective inhibition of OSBP blocks retrograde trafficking by inducing partial Golgi degradation
The dataset contains mass spectrometric raw data from two series of TMT-based expression proteomics aimed at identifying new sterol binding proteins by targeted degradation and proteins affected under the treatment of OSBP inhibitors and manganese. The dataset comprises the raw data for the 4 differ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-17 | MSV000091726 | MassIVE
ProteomicsMulti-omics analysis of the bladder and its sensitivity to inosine in a rat model of spinal cord injury
This study investigates the molecular changes in the bladder following spinal cord injury (SCI) in rats and the impact of inosine treatment using a multi-omics approach. We discovered the activation of PARP (Poly(ADP-ribose) polymerase) in response to SCI, a previously unrecognized phenomenon, and i...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-07-14 | PXD046096 | Pride
Multi-omics Spinal cord injury Inosine Bladder Rat 
ProteomicsLowering Sample Requirements to Study Tyrosine Kinase Signaling using Phosphoproteomics with the TMT Calibrator Approach
Analysis of tyrosine kinase signaling is critical for the development of targeted cancer therapy. Currently, immunoprecipitation (IP) of phosphotyrosine (pY) peptides prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to profile kinase substrates. A typical protocol requests ...
ORGANISM(S): Homo sapiens (Human) 
2020-07-29 | PXD018952 | Pride
Signaling Tmt Cancer Calibrator Tyrosine phosphorylation 
ProteomicsMulti-omics Analyses Reveal Sex Differences in Mouse Renal Proximal Tubules
Sex-dependent differences in kidney function have been recognized. However, the molecular mechanisms underlying these differences remain largely unexplored. Advances in genomics and proteomic technologies now allow for an extensive characterization of sex differences. In this study, the authors appl...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-05 | PXD037098 | Pride
Kidney Multi-omics Sex difference 
ProteomicsPRM validation of phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus
The phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus (SE), have not been detailed. In a companion project, the hippocampi of mice treated with pilocarpine and diazepam were examined by tandem mass tag (TMT11...
ORGANISM(S): Mus musculus (Mouse) 
2024-02-22 | PXD047870 | Pride
Epileptogenesis Phospho-signalling Proteomics Epilepsy Hippocampus Synaptic weakening Signalling Phosphorylation Mouse Parallel reaction monitoring Synapse growth 
ProteomicsProteomics of Rat Proximal-Tubule Cell-Culture Models
Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend on the genes expressed. New tools for transcriptomic and proteomic profiling using RNA-sequencing ...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-10-15 | PXD021160 | Pride
Proximal tubule Kideny Rat 
ProteomicsProteomic and phosphoproteomic analyses of protein PKA catalytic-alpha-null mpkCCD cells and PKA catalytic-beta-null mpkCCD cells.
Protein Kinase A (PKA) is a widely studied protein that has been viewed by most investigators as a single entity, although its catalytic subunits are coded in the genome by two separate genes, PKA catalytic alpha (Gene symbol: Prkaca) and PKA catalytic beta Gene symbol: Prkacb). At an amino-acid le...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-01 | PXD015050 | Pride
Phosphoproteomics Pka catalytic subunit Kidney Pka Mouse Proteomics Kinase Mpkccd 
Sort   by:  
 Page size