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ProteomicsDeconstruction of the GalNAc-Type O-Glycosylation Capacity of Human HEK293 Cells
Most proteins trafficking the secretory pathway of metazoan cells will acquire GalNAc-type O-glycosylation. GalNAc-type O-glycosylation is differentially regulated in cells by the expression of a repertoire of up to twenty genes encoding polypeptide GalNAc-transferase isoforms (GalNAc-Ts) that initi...
ORGANISM(S): Homo sapiens (Human) 
2019-06-24 | PXD009955 | Pride
Simplecell Mass spectrometry Glycproteomics Etd Lc-ms 
ProteomicsGlyco-DIA: a method for quantitative O-glycoproteomics with in silico-boosted glycopeptide libraries
We report a LC-MS/MS O-glycoproteomics strategy using Data Independent Acquisition (DIA) mode that holds the potential for enabling direct analysis of O-glycoproteins with characterization of sites and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries. To explor...
ORGANISM(S): Homo sapiens (Human) 
2019-11-19 | PXD011063 | Pride
Glycoproteomics Mass spectrometry O-glycosylation Data independent acquisition Human serum 
ProteomicsInstallation of O-glycan sulfation capacities in human HEK293 cells
The human genome contains at least 35 genes that encode Golgi sulfotransferases functioning in the secretory pathway, where they are involved in decorating glycosaminoglycans, glycolipids, and glycoproteins with sulfate groups. Our insight into the sulfotransferases that serve glycoproteins and in p...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD028982 | Pride
Muc1 Sulfated-t Glycosylation Sulfation Sulfated-tn O-glycans 
ProteomicsProbing the contribution of individual polypeptide GalNAc-transferase isoforms to the O-glycoproteome by inducible expression in isogenic cell lines
The GalNAc-type O-glycoproteome is orchestrated by a large family of polypeptide GalNAc-transferase isoenzymes (GalNAc-Ts) with partially overlapping contributions to the O-glycoproteome as well as distinct non-redundant functions. Increasing evidence indicate that individual GalNAc-Ts co-regulate a...
ORGANISM(S): Homo sapiens (Human) 
2018-10-16 | PXD010155 | Pride
Inducible expression Galnac-transferase O-glycosylation Glycoproteomics Mass spectrometry 
ProteomicsRole of N-glycosylation in FcRIIIa interaction with IgG
Immunoglobulins G (IgG) and their Fc gamma receptors (FcγRs) play important roles in our immune system. The conserved N-glycan in the Fc region of IgG1 impacts interaction of IgG with FcγRs and the resulting effector functions, which has led to design of antibody therapeutics with greatly improved a...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-08-30 | PXD035846 | Pride
Mabs Cd16a Igg N-glycosylation Fc gamma receptors Glycosyltransferases Surface plasmon resonance Glycoengineering 
ProteomicsExploring glycosylation of mucins with O-glycodomain reporters expressed in glycoengineered HEK293 cells
Mucins and glycoproteins with mucin-like regions contain densely O-glycosylated domains often found in tandem repeat (TR) sequences. These O-glycodomains have traditionally been difficult to characterize because of resistance to proteolytic digestion, and knowledge of positions of O-glycans is parti...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD029885 | Pride
Glycosylation Cell-based glycan array Intact mass Mucins Glycomucinases Glycosyltransferases O-glycodomains Glycoengineering 
ProteomicsIdentification of global inhibitors of cellular glycosylation
Small molecule inhibitors of glycosylation enzymes can be valuable tools for dissecting glycan functions and present great potential as drug discovery candidates. Most screening campaigns for inhibitors of glycosyltransferases have been limited to in vitro enzyme assays using recombinant enzymes, wh...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD029623 | Pride
Cell-based glycan array O-glycosylation Sars-cov-2 Compound library screen Glycosylation inhibitor 53 golgi fragmentation Glycosyltransferases 
ProteomicsMammalian Cell-Based Production of Glycans, Glycopeptides and Glycomodules
Access to defined glycans and glycoconjugates is pivotal for discovery, dissection, and harnessing of a range of biological functions orchestrated by cellular glycosylation processes and the glycome. We previously employed genetic glycoengineering by nuclease-based gene editing to develop sustainabl...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD056690 | Pride
Glycosylation Carbohydrate synthesis Mass photometry Microarray Glycoengineering 
ProteomicsDisplay of the Human Mucinome with Defined O-Glycans by Gene Engineered Cells
Mucins are a large family of heavily O-glycosylated proteins that cover all mucosal surfaces and constitute the major macromolecules in most body fluids. Mucins can form aggregated networks and bundles that serve as a barrier, but also assist in containing, feeding, clearing and replenishing microor...
ORGANISM(S): Homo sapiens (Human) 
2021-05-11 | PXD024851 | Pride
Glycosylation Orbitrap Intact mass Mucin 
ProteomicsDeconstruction of protein O-glycosylation
GalNAc-transferase (GalNAc-T) isoforms modify distinct subsets of the O-glycoproteome and GalNAc-type O-glycosylation is found on most proteins trafficking through the secretory pathway in metazoan cells. The O-glycoproteome is regulated by up to 20 polypeptide GalNAc-Ts and the contributions and bi...
ORGANISM(S): Homo sapiens (Human) 
2015-08-21 | PXD002770 | Pride
Galnt/ dimethylation/ apolipoproteins/ mass spectrometry 
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