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ProteomicsHiRIEF-LC-MS based proteomics analysis of subcellular fractions of human CD4+ T-Cells with and without T-cell Receptor stimulation.
Subcellular proteomics analysis of human T-cells in response to T-cell receptor stimulation. High Resolution Isoelectric Focusing (HiRIEF) LC-MS/MS and relative quantification by TMT 10-plex was used to analyze subcellular fractions (Cytosol, Nuclear and Mitochondria-Small Organelle-Membrane) of Hum...
ORGANISM(S): Homo sapiens (Human) 
2019-11-13 | PXD013284 | Pride
Subcellular T-cell Human T-cell receptor 
ProteomicsSubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
We have devised a simple and robust LC-MS/MS compatible protocol for subcellular protein localization studies. Here, we combine it with in depth proteome profiling and utilize the resulting dataset to predict the subcellular localization of more than 12000 proteins in 5 human cancer cell lines. The ...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD006895 | Pride
A431 Mcf7 U251 Human Cancer Hcc827 Subcellular protein localization Hirief Subcellular Nci-h322 Lc-ms. 
ProteomicsProteogenomic analysis of relapsed acute myeloid leukemia in adults and children
Despite improvement of current treatment strategies and novel targeted drugs, relapse and treatment resistance determine the major cause of death for acute myeloid leukemia (AML) patients. To identify the underlying molecular characteristics, numerous studies have been aimed to decipher the genomic-...
ORGANISM(S): Homo sapiens (Human) 
2023-01-09 | PXD031107 | Pride
Pediatric Myeloblast Human Lc-ms Leukemia Cancer Proteogenomics Clinical samples. Hirief Adult Proteomics Acute myeloid leukemia 
ProteomicsProteogenomic subtyping of chronic lymphocytic leukemia identifies subgroups with contrasting clinical outcome and distinct ex-vivo drug response profile
We have performed a comprehensive proteomic analysis of clinical patient samples of chronic lymphocytic leukemia (CLL) alongside genome-, transcriptome- and ex-vivo drug response profiling. Trisomy 12 and IGHV mutation status had the strongest impact on the proteome and transcriptome; and SF3B1 muta...
ORGANISM(S): Homo sapiens (Human) 
2022-10-17 | PXD028936 | Pride
Lymphoblast B-cell Chronic lymphoblastic leukemia Proteogenomics Human Lc-ms Leukemia Cancer Clinical samples. Hirief Proteomics 
ProteomicsProteogenomic subtyping of chronic lymphocytic leukemia identifies subgroups with contrasting clinical outcome and distinct ex-vivo drug response profile
We have performed a comprehensive proteomic analysis of clinical patient samples of chronic lymphocytic leukemia (CLL) alongside genome-, transcriptome- and ex-vivo drug response profiling. Trisomy 12 and IGHV mutation status had the strongest impact on the proteome and transcriptome; and SF3B1 muta...
ORGANISM(S): Homo sapiens (Human) 
2024-06-21 | PXD017453 | Pride
Lymphoblast B-cell Chronic lymphoblastic leukemia Proteogenomics Human Lc-ms Leukemia Cancer Clinical samples. Hirief Proteomics 
ProteomicsProteogenomics of ex vivo-activated platelets.
Platelets are major players in the process of intravascular thrombus formation. Current therapeutic strategies still fail to prevent thrombotic coronary events in a substantial number of patients, indicating that the complex mechanisms modulating platelet response during activation are not fully elu...
ORGANISM(S): Homo sapiens (Human) 
2018-01-03 | PXD006559 | Pride
Human Proteome Platelet activation Cytoplasmic pre-mrna splicing 
ProteomicsHiRIEF-LC-MS based proteomics on Small Intestinal Neuroendocrine Tumors (SI-NET) and SI-NET cell lines
Associated to PXD009877 which contains Part I and Part II. Part III – Pevonedistat/MLN4924 and Bortezomib treatment of the SI-NET (small intestinal – neuroendocrine tumor) cell line CNDT2. High Resolution Isoelectric Focusing (HiRIEF) LC-MS and relative quantification by TMT 10-plex was used to an...
ORGANISM(S): Homo sapiens (Human) 
2021-06-10 | PXD013024 | Pride
Human Pevonedistat Neuro endocrine tumor Bortezomib Cell line 
ProteomicsSubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
We have devised a simple and robust LC-MS/MS compatible protocol for subcellular protein localization studies. Here, we combine it with in depth proteome profiling and utilize the resulting dataset to predict the subcellular localization of more than 12000 proteins in 5 human cancer cell lines. The ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-17 | MSV000086899 | MassIVE
Proteomics,MultiomicsHiRIEFII proteomics and proteogenomics of A431 cells and five histologically normal tissues
High Resolution Isoelectric Focusing (HiRIEF) LC-MS was used to analyze samples from the human A431 cell line and also samples of histologically normal tissues. The high analytical depth afforded by HiRIEF permitted proteogenomics on these two sample sets (A431 cells and normal tissues). The A431 d...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-10-04 | MSV000083000 | MassIVE
ProteomicsSubCellBarCode: Integrated workflow for robust classification and visualization of spatial proteome
We have developed a mass spectrometry (MS) and bioinformatics-based pipeline to generate a proteome-wide resource of protein subcellular localization across multiple human cancer cell lines (www.subcellbarcode.org). Here, we present a detailed wet-lab protocol spanning from subcellular fractionation...
ORGANISM(S): Homo Sapiens (human) 
2022-09-26 | PXD022533 | JPOST Repository
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