Sort   by:  
 Page size 
ProteomicsMapping protein-DNA interactions by UV femtosecond laser crosslinking and mass spectrometry analysis
Transcription factors (TFs) regulate target genes by specific interaction with DNA sequences. Detecting and understanding these interactions on molecular level is of fundamental interest in both biological and clinical context. Crosslinking mass spectrometry is a powerful tool to assist structure pr...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Sus scrofa domesticus (domestic pig) 
2020-06-22 | PXD014898 | Pride
Femtosecond laser Uv Transcription factor Protein-dna crosslinking Mass spectrometry 
ProteomicsRBPome of primary human T helper cells
Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD021164 | Pride
Human Rna interaction capture Rna binding proteins 
ProteomicsRBPome of primary human T helper cells
Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD021169 | Pride
Human Oops Rna binding proteins 
ProteomicsRBPome of primary mouse T helper cells
Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about ...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-01 | PXD022795 | Pride
Mouse Oops Rna binding proteins 
ProteomicsChIP-MS of Glucocorticoid Receptor in primary mouse macrophages
Glucocorticoids (GCs) are the most effective anti-inflammatory drugs in current clinical settings, yet their genomic modes of action are poorly understood. GCs bind to the Glucocorticoid Receptor (GR), which acts as a transcription factor to control gene expression in the immune system. Understandin...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD018077 | Pride
Epigenetics Immunology Transcription factor Chromatin 
ProteomicsChIP-MS of GR and GR-DZn in mouse embryonic fibroblasts
The Glucocorticoid Receptor (GR) is both one of the most widely used clinical drug targets and a very potent metabolic regulator. GR belongs to the nuclear hormone receptor family of ligand-gated transcription factors that govern mammalian physiology. Upon ligand binding, GR enters the nucleus to re...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-07 | PXD013772 | Pride
Coregulator Hormone Steroid receptor Chromatin 
ProteomicsCompartment-resolved proteomic analysis of mouse aorta during atherosclerotic plaque formation
Atherosclerosis leads to vascular lesions that involve major rearrangements of the vascular proteome, especially of the extracellular matrix (ECM). Using single aortas from ApoE knock out mice, we quantified formation of plaques by single-run, high-resolution mass spectrometry (MS)-based proteomics....
ORGANISM(S): Mus musculus (Mouse) 
2017-12-08 | PXD006752 | Pride
Ecm Mouse model Atherosclerosis Apoe Qdsp 
ProteomicsAP-MS of EPOP in mouse embryonic stem cells
A Flag-tagged version of murine EPOP was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.
ORGANISM(S): Mus musculus (Mouse) 
2016-12-13 | PXD005082 | Pride
Esc Prc2 Polycomb 
ProteomicsAP-MS of Elongin B in mouse embryonic stem cells
A Flag-tagged version of murine Elongin B was expressed in mouse embryonic stem cells (ESCs) at near to endogenous levels and purified using an anti-FLAG affinity matrix.
ORGANISM(S): Mus musculus (Mouse) 
2016-12-13 | PXD005081 | Pride
Esc Elob Prc2 Tceb2 Polycomb 
ProteomicsPhosphoproteome of MCF7 breast cancer cells treated with estradiol and BI2536
Triple SILAC phosphoproteome experiment of hormone deprived MCF7 cells treated with estradiol (E2) in presence or absence of the PLK1 inhibitor BI2536. Conditions were as follows: light (L) population: 1h DMSO, 30min EtOH; medium (M) population: 1h DMSO, 30min E2; heavy (H) population: 1h BI2536, 3...
ORGANISM(S): Homo sapiens (Human) 
2016-06-24 | PXD000275 | Pride
Mcf7 Silac Phosphorylation Gene regulation Plk1 Phosphoproteomics Bi2536 Estradiol 
Sort   by:  
 Page size