Sort   by:  
 Page size 
ProteomicsMapping protein-DNA interactions by UV femtosecond laser crosslinking and mass spectrometry analysis
Transcription factors (TFs) regulate target genes by specific interaction with DNA sequences. Detecting and understanding these interactions on molecular level is of fundamental interest in both biological and clinical context. Crosslinking mass spectrometry is a powerful tool to assist structure pr...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Sus scrofa domesticus (domestic pig) 
2020-06-22 | PXD014898 | Pride
Femtosecond laser Uv Transcription factor Protein-dna crosslinking Mass spectrometry 
ProteomicsRBPome of primary human T helper cells
Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD021164 | Pride
Human Rna interaction capture Rna binding proteins 
ProteomicsRBPome of primary human T helper cells
Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-01 | PXD021169 | Pride
Human Oops Rna binding proteins 
ProteomicsRBPome of primary mouse T helper cells
Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about ...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-01 | PXD022795 | Pride
Mouse Oops Rna binding proteins 
ProteomicsStreamlined identification of permissive non-canonical amino acid incorporation sites in mammalian cell lines with an expanded genetic code
The genetic code of mammalian cells can be expanded to allow the incorporation of non-canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an orthogonal pyrrolysyl-tRNA synthetase (PylRS)/tRNAPylCUA (PylT) pair. However, the feasibility of this approach is substantially...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2021-02-23 | PXD019815 | Pride
Genetic code expansion Unnatural amino acids Non-canonical amino acids Amber suppression 
ProteomicsChIP-MS of DPPA3/STELLA in mouse embryonic stem cells
Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. So far, it was unclear how mammals specifically achieve global DNA hypomethylation, given the high conservation of the DNA (de-)methylation machinery among vertebrates. We found that DNA deme...
ORGANISM(S): Mus musculus (Mouse) 
2020-10-12 | PXD019794 | Pride
Epigenetics Dna methylation Escs Chip-ms 
ProteomicsAP-MS of replisomes from S. cerevisiae in S phase and after unscheduled G1 replication
Interfering with once-per-cell-cycle regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineered different systems in budding yeast to induce over-replication in G1 and investigated its ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-11-09 | PXD028308 | Pride
Baker’s yeast Cell division cycle Replication initiation Re-replication Replication stress Replisome 
ProteomicsChIP-MS of Glucocorticoid Receptor in primary mouse macrophages
Glucocorticoids (GCs) are the most effective anti-inflammatory drugs in current clinical settings, yet their genomic modes of action are poorly understood. GCs bind to the Glucocorticoid Receptor (GR), which acts as a transcription factor to control gene expression in the immune system. Understandin...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD018077 | Pride
Epigenetics Immunology Transcription factor Chromatin 
ProteomicsChIP-MS for GR in liver from wild type and E47 mutant mice
Glucocorticoids (GCs) are widely prescribed effective drugs, but their clinical use is compromised by severe side effects including hyperglycemia, hyperlipidemia and obesity. They bind to the Glucocorticoid Receptor (GR), which acts as a ligand-gated transcription factor. The transcriptional activat...
ORGANISM(S): Mus musculus (Mouse) 
2018-11-22 | PXD010157 | Pride
Coregulator Hormone Steroid receptor Chromatin 
ProteomicsChIP-MS of GR and GR-DZn in mouse embryonic fibroblasts
The Glucocorticoid Receptor (GR) is both one of the most widely used clinical drug targets and a very potent metabolic regulator. GR belongs to the nuclear hormone receptor family of ligand-gated transcription factors that govern mammalian physiology. Upon ligand binding, GR enters the nucleus to re...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-07 | PXD013772 | Pride
Coregulator Hormone Steroid receptor Chromatin 
Sort   by:  
 Page size