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ProteomicsComparative interactome analysis of Nematostella vectensis Hsp70 isoforms reveals unique clientome remodeling upon heat stress.
Comparative interactome analysis of Nematostella vectensis Hsp70 isoforms reveals unique clientome remodeling upon heat stress. Three isoforms of nvHSP70 (A, B, and D) were IP'd from either Heat Shock conditions (HS) or non-Heat Shock (UN) and their interactomes were compared. Mass Spec was run on...
ORGANISM(S): Nematostella vectensis Saccharomyces cerevisiae (Baker's yeast) 
2019-06-25 | PXD012144 | Pride
Nvhsp70 Q-exactive Heat shock Qe Interactome Lfq quantification 
ProteomicsChemotherapy-induced cellular senescence is characterized by aberrant lipid regulation and lipid peroxidation
As normal cells approach their proliferative limit, they arrest to undergo replicative senescence, displaying large cell size, flat morphology and activation of senescence-associated beta-galactosidase (SA-b-Gal). A subset of normal or tumor cells exposed in vitro or in vivo to chemotherapy agents s...
ORGANISM(S): Mus musculus (Mouse) 
2017-11-06 | PXD006216 | Pride
Maxquant lfq Lipid peroxidation Mus Q-exactive Chemotherapy-induced senescence Quant proteomics 
ProteomicsTopoisomerase I Poisoning by Electrophiles Derived from Oxidative Stress
The cancer target enyzme topoisomerase 1 transiently cleaves one strand of chromosomal DNA to relax accumulated strain that can prevent transcription, replication or chromatin assembly. The topoisomerase 1 poison camptothecin and its synthetic analogs are widely used chemotherapeutics that act by tr...
ORGANISM(S): Homo sapiens (Human) 
2021-03-24 | PXD018549 | Pride
Oxidative stress Chemotherapy Q-exactive Topoisomerases Enzymology Proteomics 
ProteomicsSubcellular localization of the J-protein Sis1 regulates the heat shock response
How heat shock inducesthe heat shock response (HSR) – a gene expression program encoding chaperones and other protein homeostasis (proteostasis) factors –remains an unresolved question in eukaryotic cell biology. Here we show that subcellular localization of the conserved J-protein Sis1 is a key reg...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-24 | PXD019170 | Pride
Hsf1 Heat shock Q-exactive Saccharomyces cerevisiae Sis1 J-protein 
ProteomicsPost-Translational Modifications of recombinant Hsp70s expressed in different systems can alter their functional properties
Post-Translational Modifications of recombinant Hsp70s expressed in different systems can alter their functional properties
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Mycobacterium tuberculosis GM 1503 
2020-03-13 | PXD011583 | Pride
Hsp70 Qe Post-translational modifications Ptm proteomics 
ProteomicsGenetic analysis of Hsp70 phosphorylation sitesreveals a role in Candida albicanscell and colony morphogenesis
Heat shock proteins are best known for their role as chaperonins involved in general proteostasis, but they can also participate in specific cellular regulatory pathways, e.g. via their post-translational modification. Hsp70/Ssa1 is a central cytoplasmic chaperonin in eukaryotes, which also particip...
ORGANISM(S): Candida albicans (Yeast) 
2018-11-21 | PXD010827 | Pride
Candida albicans Hsp70 Mass spectrometry Q-exactive Post-translational modification Colony morphology Lfq quantification. 
ProteomicsMevalonate pathway activity is a key determinant of radiation sensitivity in head and neck cancer
Radioresistance is a major hurdle in the treatment of head and neck squamous cell carcinoma (HNSCC). Here we report a novel role for statin-based treatment of HNSCCs as an actionable and safe adjuvant to radiotherapy. Proteomic profiling and comparison of radiosensitive and radioresistant HNSCCs rev...
ORGANISM(S): Homo sapiens (Human) 
2019-06-25 | PXD007188 | Pride
Q-exactive Hnscc Pitavastatin Ddr Senescence Mevalonate pathway Lfq quantification. 
ProteomicsInactivation of a NB-LRR plant immune-effector complex by post-translational modification
Modification of plant immune complexes by pathogen effectors can trigger strong immune responses mediated by the action of nucleotide binding-leucine rich repeat immune receptors. Although some strains of the pathogen Pseudomonas syringae harbor effectors that individually can trigger immunity, the ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Pseudomonas syringae 
2016-05-04 | PXD001344 | Pride
Mass spectrometry Arabidopsis thaliana Q-exactive Pseudomonas syringae Acetylation 
ProteomicsCUX1 recruits SWI/SNF to promote chromatin accessibility in human hematopoietic cells
Transcription factors(TF) plays a key role in hematopoiesis via promoting cell-type specific gene expression. A subset of TFs, canonically termed “pioneer factors”, possesses the ability to bind to otherwise inaccessible heterochromatin regions, and recruit additional TFs, co-activators, and chromat...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD037838 | Pride
Human Q-exactive Ip-ms Cux1 Swi/snf Chromatin 
ProteomicsRapid acetylation of yeast Hsp70 mediates the cellular response to heat stress
Hsp70 is a highly conserved molecular chaperone critical for the folding of new and denatured proteins. While traditional models state that cells respond to stress by upregulating inducible HSPs, this response is relatively slow and is limited by transcriptional and translational machinery. Recent s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-02-24 | PXD015185 | Pride
Mass spectrometry Ptms Q-exactive Yeast Ssa1 Heat stress Acetylation 
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