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ProteomicsA deeper look at carrier proteome effects for single-cell proteomics
We probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at h...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2022-02-24 | PXD027742 | Pride
Tmt Proteomics Single cell 
ProteomicsOne-Tip enables comprehensive proteome coverage in minimal cells and single zygotes
We present One-Tip, a lossless proteomics methodology that seamlessly combines swift, one-pot sample preparation with narrow-window data-independent acquisition mass spectrometric analysis. With simplest sample processing, One-Tip reproducibly identifies > 9,000 proteins from ~1000 cells and ~ 6,000...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-09-15 | PXD044991 | Pride
Astral Proteomics Zygote 
ProteomicsEvolutionarily-conserved FOXO-regulated OSER1 extends lifespan and healthspan
Molecular aging reflects the time-dependent accumulation of cellular damage and loss of essential cellular functions. FOXO transcription factors and their downstream targets play critical roles in the response to aging-associated cellular damage. Here, we identify and characterize a novel FOXO-regul...
ORGANISM(S): Homo sapiens (Human) 
2024-07-25 | PXD036579 | Pride
Oser1 Lifespan 
ProteomicsDissecting the functional selectivity of TGFβ signaling pathway components using genome engineered human organotypic skin models
Transforming growth factor β (TGFβ) signaling is essential in cell growth and differentiation. Yet, the role of the individual TGFβ signaling components in human tissue homeostasis and transformation is still incompletely understood. Here we dissected the importance of the core components in the TGF...
ORGANISM(S): Homo sapiens (Human) 
2023-02-09 | PXD024935 | Pride
Keratinocyte Proteomics Smad transcription factor Transforming growth factor beta (tgfβ) Organotypic skin culture Crispr/cas9 Phosphoproteomics 
ProteomicsEnhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics
We present a high-sensitivity SCP workflow named Chip-Tip, identifying >5000 proteins in individual HeLa cells
ORGANISM(S): Homo Sapiens 
PXD054944 |
ProteomicsGalectin-1 upregulates IDO1 and PD-L1 and induces a tolerogenic tumor-associated M2 macrophage phenotype
Galectins are a group of carbohydrate binding proteins with immunomodulatory functions. While the effects of galectins on the T cell compartment are well described, the interactions between galectins and antigen-presenting cells (APCs) remain elusive. Here we find increased expression of galectin-1(...
ORGANISM(S): Homo sapiens (Human) 
2023-08-14 | PXD034623 | Pride
Galectin Macrophage Ido1 Pd-l1/cd274 Lc-ms/ms 
ProteomicsEnhanced Proteomic Mapping of Arginine Methylation Using Electron Transfer Dissociation Reveals Novel PRMT5 Substrates and Arginine Trimethylation
PRMT5 is the major enzyme that catalyzes the symmetric dimethylation of arginine (sDMA) in mammalian cells. Its activity is crucial for many biological processes including transcriptional regulation, mRNA processing, as well as DNA damage and repair. However, the protein substrates identified for PR...
ORGANISM(S): Homo sapiens (Human) 
2024-04-30 | PXD037425 | Pride
Prmt5 Etd Methylation Proteomics 
ProteomicsNarrow-window DIA: Ultra-fast quantitative analysis of comprehensive proteomes with high sequencing depth. Evaluation of window width and injection time.
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes in a fast and reproducible manner. Here, we present an ultra-fast scanning data-independent acquisition (DIA) strategy consisting on 2-Th precursor isolation windows, dissolving the differences between data-dependen...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000095977 | MassIVE
ProteomicsThe research of RBM39 in multiple myeloma
RBM39 is a RNA binding protein, we aim to investigate its role in multiple myeloma. We performed mass spectrometry of RBM39 interacted protein obtained by IP.
ORGANISM(S): Homo Sapiens 
2025-02-09 | PXD060610 |
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