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ProteomicsMutations in disordered regions cause disease by creating dileucine motifs - PRM dataset-
Mutations in intrinsically disordered regions (IDRs) of proteins are associated with a wide spectrum of diseases. Since IDRs lack a fixed three-dimensional structure, the molecular mechanism by which such mutations cause disease is often unknown. Here, we employ a peptide-based proteomic screen to i...
ORGANISM(S): Homo sapiens (Human) 
2018-09-13 | PXD010005 | Pride
Glut1 Disordered regions Prm Dileucin 
ProteomicsRPL12 phosphorylation regulates translation during mitosis - pSILAC B cell PRM -
project_description Emerging evidence indicates that heterogeneity in ribosome composition can give rise to specialized functions. Until now, research mainly focused on differences in core ribosomal proteins and associated factors. The impact of posttranslational modifications has not yet been studi...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-28 | PXD010029 | Pride
Prm Polysome proteome profiling B cell Mouse 
ProteomicsPicky - a simple online method designer for targeted proteomics
Picky is an online method designer for targeted proteomics. To assess the performance of PRM methods designed by Picky we carried out a benchmark experiment. As reference samples we used different amounts of human proteins spiked into 1.4 µg yeast lysate. These sample where analyzed in PRM and DDA m...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2017-08-04 | PXD007039 | Pride
Prm Benchmark Dda 
ProteomicsThe natural diversity of the yeast proteome links aneuploidy tolerance to protein turnover
Accessing the natural genetic diversity of species unveils hidden genetic traits, clarifies gene functions, and allows assessment of the generalizability of laboratory findings. One notable discovery made in Saccharomyces cerevisiae natural isolates is frequent (~20%) aneuploidy – an imbalance in ch...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-03-13 | PXD048219 | Pride
Aneuploidy Protein stability Yeast Silac 
ProteomicsComprehensive computational identification and characterization of conserved small ORFs in animals
There is increasing evidence that transcripts or transcript regions annotated as non-coding can harbor functional short open reading frames (sORFs). Loss-of-function experiments have identified essential developmental or physiological roles for a few of the encoded peptides (micropeptides), but geno...
ORGANISM(S): Mus musculus (Mouse) 
2015-07-28 | PXD002583 | Pride
Mouse Sorfs Micropeptides Functional small open reading frames 
ProteomicsPicky – a simple online method designer for targeted proteomics
The accuracy of the retention time prediction build into picky was tested on a digested HeLa sample analyzed with a standard data-dependend acquisition method (top20).
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2017-12-13 | PXD008212 | Pride
Prm Retention-time Srm 
ProteomicsThe UBA-domain of Ubc1/Ube2K Facilitates the Assembly of K48-/K63-Branched Ubiquitin Chains
Assembly of polymeric ubiquitin (Ub) chains is an essential posttranslational protein modification that regulates widespread intracellular processes in eukaryotic cells. Factors and mechanisms that regulate the formation of Ub chains are key to the understanding of many cellular processes. The E2 en...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD018651 | Pride
Ubc1/ube2k ubiquitin k48-/k63-branched ubiquitin prm 
ProteomicsThe UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains
The assembly of a specific polymeric ubiquitin (Ub) chain on a target protein is a key event in the regulation of numerous cellular processes. Yet, the mechanisms that govern the selective synthesis of particular polyubiquitin signals remain enigmatic. The ubiquitin-conjugating (E2) enzymes Ubc1 in ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-02 | PXD022621 | Pride
Ubiquitin Ubc1 Ubc4 Yeast Branched 
ProteomicsHuman Proteome Dataset Hek293, k652 and HeLa cell lines
Triple Silac approach to analyze cellline specific protein expression. Hek293 (Heavy, Lys8 and Arg10), K526 (Medium, DMEM with Lys 4 and Arg6), HeLa (Light).
ORGANISM(S): Homo sapiens (Human) 
2015-07-08 | PXD002383 | Pride
Hek293 Hela K652 
ProteomicsDisintegrin-and-metalloproteinase-19 cleaves parathyroid hormone receptor and could cause brachydactyly type E
Brachydactyly type E (BDE), shortened metacarpals, metatarsals, cone-shaped epiphyses, and short stature, is an autosomal-dominant condition that commonly occurs as a sole phenotype. Parathyroid hormone-like protein (PTHrP) has been shown to be responsible in all forms to date, either directly or in...
ORGANISM(S): Homo sapiens (Human) 
2026-07-07 | PXD044793 | Pride
Human Parathyroid receptor Brachydactyly type e Shotgun proteomics Disintegrin-and-metalloproteinase-19 Lc-msms Gfp Parathyroid-related peptide 
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