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Proteomicshuman ESCO2-interacting proteins in response to DNA damage
We used IP-MS to detected the proteins that interact with ESCO2 in response to DNA damage.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039072 | Pride
Huamn Esco2 Dna damage 
ProteomicsDeubiquitination of CDC6 by OTUD6A promotes tumour progression and chemoresistance
CDC6 is an oncogenic protein whose expression level fluctuates during the cell cycle. Although several E3 ubiquitin ligases responsible for the ubiquitin-mediated proteolysis of CDC6 have been identified, the deubiquitination pathway for CDC6 has not been investigated. Here, we report that OTUD6A fu...
ORGANISM(S): Homo sapiens (Human) 
2024-05-24 | PXD042935 | Pride
Otud6a Deubiquitinase Hek293 
ProteomicsPancreatic cancer-associated proteome sequencing
In this study, 30 pancreatic tissues were retrieved, including 20 samples of pancreatic ductal cell carcinoma and 10 of adjacent healthy tissues. All pancreatic samples were obtained from the First Hospital of Lanzhou University from January 2016 to December 2020.
ORGANISM(S): Homo Sapiens 
2023-08-11 | PXD044500 |
ProteomicsIdentification of antiviral RNAi regulators, ILF3/DHX9, recruit at ZIKV stem loop B to protect against ZIKV induced microcephaly
The stained gel bands are washed three times with ddH2O, followed by the addition of 150μL of destaining solution to remove the stain. After four rounds of water washing, the gel bands are washed twice with 300μL each of 25mM ammonium bicarbonate, 50% acetonitrile, and 100% acetonitrile, respectivel...
ORGANISM(S): Homo Sapiens 
2024-08-14 | PXD054904 |
Proteomics2022-0222-SK cell line infected by ZIKV（MR766,PRVABC.59)-1044
In this project, mass spectrometer Q-Exactive HF X was used. Total number of 43898 spectra were obtained from the sample group SK_mock. After identification by search engine, 6733 spectra were matched, 1094 proteins and 5074 peptides were identified.Total number of 46943 spectra were obtained from t...
ORGANISM(S): Homo Sapiens 
2024-03-25 | PXD050964 |
ProteomicsCo-IP and LC-MS/MS analysis for probing the binding partners of ITGBL1 in fibroblasts
Co-IP, LC-MS/MS and cluster analysis of the binding proteins for targeted ITGBL1 protein using the ITGBL1 antibody or control IgG in ITGBL1-overexpressing WI-38 cells.
ORGANISM(S): Homo Sapiens 
2020-01-19 | PXD017182 |
ProteomicsLow glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate
This project identified the interacting proteins of p53 under glucose starvation in HEK293 cells.
ORGANISM(S): Homo Sapiens 
2023-04-04 | PXD041361 |
Proteomicsm6A binding proteins identified by SPIDER assay
To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs（with and withou...
ORGANISM(S): Homo Sapiens 
2022-11-02 | PXD026519 |
ProteomicsSide-by-side comparison of AP-MS, Tri-functional affinity probe, BioID, PUP-IT and SPIDER to identify CheZ-interacting proteins
Comparison of CheZ interactors’ ratio of two replicate experiments using five different methods, i.e., Affinity-Purification, Tri-functional affinity probe Sulfo-SBED and proximity labeling methods BioID, SPIDER, PUP-IT
ORGANISM(S): Escherichia Coli 
2022-11-03 | PXD037952 |
ProteomicsCobB interacting proteins identified by SPIDER assay
To assess whether SPIDER could detect transient interactions such as enzymes and their substrates, we examined the interactome of the E. coli protein deacetylase CobB. As the only member of the Sir2 family of deacetylases in E. coli, CobB is known to play a role in many different pathways but their ...
ORGANISM(S): Escherichia Coli 
2021-06-04 | PXD026514 |
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