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ProteomicsAccelerated Lysis and Proteolytic Digestion of Biopsy-level Fresh Frozen and FFPE Tissue Samples Using Pressure Cycling Technology
We developed an accelerated PCT method with doubled throughput while maintaining digestion efficiency, the number of proteins/peptides identified, as well as reproducibility of protein quantitation, compared to the previously reported method. The accelerated PCT method does not require new chemical ...
ORGANISM(S): Homo Sapiens 
PXD020353 |
UnknownMicrodroplet Mass Spectrometry Enables Extremely Accelerated Pepsin Digestion of Proteins.
Not available
S-EPMC8267842 | biostudies-literature
ProteomicsRevisiting the effect of trypsin digestion buffers on artificial deamidation
Deamidation of asparagine and glutamine residues occurs spontaneously, accelerated by adjacent amino acid residues and influenced by pH, temperature, and incubation time. Standard proteolytic digestion induces significant deamidation, which is especially problematic in bottom-up proteomic studies. T...
ORGANISM(S): Mus musculus (Mouse) 
2025-03-11 | PXD054603 | Pride
De-glycopeptides Chemical deamidation De-glycoproteomics 
ProteomicsRapid trypsin digestion at elevated temperature with calcium for enhanced bottom-up proteome analysis
Trypsin is frequently employed to cleave proteins ahead of mass spectrometry characterization. Traditionally, enzyme digestion involves overnight incubation of proteins at 37 °C, which is not only time consuming but can still yield in poor digestion efficiency. While raising the temperature should t...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Bacteria 
2023-03-11 | PXD035682 | Pride
Trypsin  calcium  bottom-up proteomics  mass spectrometry  accelerated digestion  digestion efficiency  quantitative proteomics  sample preparation 
ProteomicspSILAC method coupled with two complementary digestion approaches reveals PRPF39 as a new E7070-dependent DCAF15 substrate
In this study, we used a novel strategy of pSILAC method coupled with two complementary digestion approaches to find potential substrates of E7070, and reveals PRPF39 as E7070-dependent DCAF15 substrate.
ORGANISM(S): Homo Sapiens 
2019-10-18 | PXD015892 |
ProteomicsNAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome
Metabolic dysfunction is a primary feature of Werner syndrome (WS), a human premature aging disease caused by mutations in the gene encoding the Werner (WRN) DNA helicase. WS patients exhibit severe metabolic phenotypes, but the underlying mechanisms are not understood, and whether the metabolic def...
ORGANISM(S): Caenorhabditis elegans 
2020-01-13 | PXD015644 | Pride
Mitophagy Wrn Nad+ Nmnat1 Aging 
ProteomicsChlorella vulgaris Protein Isolate Effectively Protects Lacticaseibacillus rhamnosus GG Viability During processing, Storage, and In vitro Digestion
This study examined the protective effect of Chlorella vulgaris protein isolate (CPI) on the biological activity of Lacticaseibacillus rhamnosus GG (LGG) during lyophilisation, storage, and in vitro digestion. Prior to lyophilisation, probiotic hydrogels were either fermented to pH 4.5 (CF) or left ...
ORGANISM(S): Chlorella vulgaris (Green alga) Lactobacillus rhamnosus GG 
2026-01-06 | PXD066171 | Pride
Probiotics Encapsulation Microalgae Protein isolate Viability In vitro digestion 
ProteomicsScleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype
Aged tendons have disrupted homeostasis, increased injury risk, and impaired healing capacity. Understanding mechanisms of homeostatic disruption is crucial for developing therapeutics to retain tendon health through the lifespan. Here, we developed a novel model of accelerated tendon extracellular ...
ORGANISM(S): Mus musculus (Mouse) 
2023-02-03 | PXD037230 | Pride
Extracellular matrix Tendon Aging Mouse Scleraxis Lc-ms/ms 
UnknownIn planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion.
Not available
S-EPMC5597601 | biostudies-literature
ProteomicsPhysiological and proteomic changes in soft wheat seeds during accelerated aging
Wheat (Triticum aestivum L.), one of the most important crops in the world, is a staple food used for making flour, noodles, alcoholic beverages, biofuel and a variety of other products. In wheat, kernel texture and biochemical composition vary with different hardnesses. Previous study of our resear...
ORGANISM(S): Triticum aestivum (Wheat) 
2018-10-08 | PXD009156 | Pride
Tandem mass tag Differentially expressed proteins Wheat Lc-ms/ms 
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