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MetabolomicsMetabolome analysis reveal key regulatory pathways of feed conversion efficiency of oriental river prawn Macrobrachium nipponense

A total of 445 and 247 differently expressed metabolites (DEMs) were identified in the hepatopancreas and muscle. In addition, 11 KEGG pathways, which mainly include lysine biosynthesis, lysine degradation, aminoacyl-tRNA biosynthesis and purine metabolism, were sign...

2023-02-28 | MTBLS6663 | MetaboLights
ProteomicsTandem LysC/trypsin digestion performs efficient in detergent conditions and under constant denaturating environments
All shotgun proteomics experiments rely on efficient proteolysis steps for sensitive peptide/protein identification and quantification. Continuous protocol improvement is therefore a constant topic in proteomics research. Previous reports suggest that the sequential tandem LysC/trypsin digest yields...
ORGANISM(S): Escherichia coli Bacteria 
2020-04-30 | PXD013273 | Pride
Shotgun proteomics Protein digestion Lysc Trypsin 
ProteomicsDigestion time course of human liver plasma membrane
The selection of signature peptides is critical for reliable peptide quantification. The purpose of this study was to comprehensively assess the digestion efficiency and stability of tryptic peptides and to identify optimal signature peptides for human hepatic transporters and membrane marker protei...
ORGANISM(S): Homo Sapiens (human) 
2022-10-12 | PXD034402 | JPOST Repository
ProteomicsRapid trypsin digestion at elevated temperature with calcium for enhanced bottom-up proteome analysis
Trypsin is frequently employed to cleave proteins ahead of mass spectrometry characterization. Traditionally, enzyme digestion involves overnight incubation of proteins at 37 °C, which is not only time consuming but can still yield in poor digestion efficiency. While raising the temperature should t...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Bacteria 
2023-03-11 | PXD035682 | Pride
Trypsin  calcium  bottom-up proteomics  mass spectrometry  accelerated digestion  digestion efficiency  quantitative proteomics  sample preparation 
ProteomicsHydroxylamine Chemical Digestion for Insoluble Extracellular Matrix Characterization
The extracellular matrix (ECM) is readily enriched by decellularizing tissues with non-denaturing detergents to solubilize and deplete the vast majority of cellular components. This approach has been used extensively to generate ECM scaffolds for regenerative medicine technologies and in 3D cell cul...
ORGANISM(S): Mus musculus (Mouse) 
2017-10-20 | PXD006428 | Pride
Proteomics Mass spectrometry Lc-srm Matrisome Tissue extraction Collagen Chemical digestion Extracellular matrix 
ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
ProteomicsAssessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics
In this study, we evaluated three well-established sample preparation methods for shotgun proteomics, named: i) UREA; ii) sodium dodecyl sulfate (SDS); and iii) filter-aided sample preparation (FASP) protocols. We focused our analysis on protein extraction and digestion from heart left ventricle (LV...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-05-21 | PXD012591 | Pride
Lc-msms Rat Dimethyl labeling Heart Fasp 
Genomicsanaerobic digestion
Ammonium influences microbial community composition in anaerobic digestion of swine manure
PRJEB14681 | ENA
Genomicsanaerobic digestion
Ammonium influences microbial community composition in anaerobic digestion of swine manure
PRJEB14682 | ENA
UnknownQuantitative assessment of in-solution digestion efficiency identifies optimal protocols for unbiased protein analysis.
Not available
S-EPMC3790306 | biostudies-literature
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