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ProteomicsMultiple protease based human phosphopeptide atlas
Site-specific phospho-antibodies are commonly used to study these changes, however only a limited number of good antibodies is available, covering just a few signaling nodes in important pathways. It is highly unlikely that specific antibodies, covering the more than hundred thousands of estimated p...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-04 | MSV000080223 | MassIVE
ProteomicsCharacterization of phosphopeptide enrichment strategy for mass spectrometry-based proteomic analysis
We attempted to evaluate four strategies for phosphopeptide enrichment, using the titanium dioxide (TiO2) and Phos-tag ligand particles, from in-solution or in-gel digests prior to mass spectrometry-based proteomic analysis.
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2021-12-24 | PXD026295 | JPOST Repository
ProteomicsRobust, reproducible, and economical phosphopeptide enrichment using calcium titanate.
We compare different preparations of TiO2 for phosphopeptide enrichment and demonstrate the efficacy of a method using CaTiO3
ORGANISM(S): Homo sapiens (Human) 
2019-01-02 | PXD011145 | Pride
Phosphoproteomics Enrichment Phosphopeptide 
ProteomicsComprehensive evaluation of different TiO2-based phosphopeptide enrichment and fractionation methods for phosphoproteomics
We performed a comprehensive evaluation of four different TiO2-based phosphopeptide enrichment methods using different non-phosphopeptide excluders. Then, two phosphopeptide fractionation methods, including the ammonia-based and the TEA based high pH reversed-phase fractionation, are also evaluated.
ORGANISM(S): Homo Sapiens 
PXD034264 |
ProteomicsSimulated phosphopeptide spectral library for confident site localization
We have developed a new workflow to unambiguously localize phosphorylation sites on proteins. We demonstrate that spectral matching of phosphopeptide datasets against a library of the well-simulated spectra provided higher sensitivity for confident site localization than other tested programs. To co...
ORGANISM(S): Homo sapiens (Human) 
2015-03-17 | PXD000474 | Pride
Synthetic peptide Simulated spectral library Hela Phosphopeptide Lc-ms/ms 
ProteomicsHuman brain phosphoproteome analyzed by refined phosphopeptide enrichment and high resolution LC-MS/MS
Alzheimer’s disease (AD) is the most common form of dementia, characterized by progressive loss of cognitive function. One of the pathological hallmarks of AD is the formation of neurofibrillary tangles composed of abnormally hyperphosphorylated tau protein, but global deregulation of protein phosph...
ORGANISM(S): Homo sapiens (Human) 
2015-01-28 | PXD001180 | Pride
Phosphorylation Human brain Alzheimer's disease Phosphopeptide enrichment Lc-ms/ms 
GenomicsTranscriptomic and Metabolomic Changes Reveals the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs
Transcriptomic and Metabolomic Changes Reveals the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs
PRJNA918394 | ENA
UnknownIdentification of a major phosphopeptide in human tristetraprolin by phosphopeptide mapping and mass spectrometry.
Not available
S-EPMC4091943 | biostudies-literature
ProteomicsAn Internal Standard for Assessing Phosphopeptide Recovery from Metal Ion/Oxide Enrichment Strategies
Phosphorylation-mediated signaling pathways have major implications in cellular regulation and disease. Mass spectrometry-based proteomics is a well-established approach for quantifying thousands of phosphorylation events in a single experiment. However, proteins with roles in signaling pathways are...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-12-04 | PXD008966 | Pride
Tmt super-heavy Lumos Phosphorylation Mtraq Tmtzero Tmtsh Tmt0 
ProteomicsPhosphopeptide enrichment on synthetic libraries and HeLa cells digest
2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-11 | PXD000759 | Pride
Phosphopeptide enrichment Tio2 Ti-imac Hela Synthetic library 
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