Sort   by:  
 Page size 
ProteomicsA Suspension Trapping-based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2023-08-28 | PXD039933 | JPOST Repository
ProteomicsSuspension trapping (S-trap) is compatible with typical protein extraction buffers and detergents for bottom up proteomics
Analysis of cells and tissue by bottom up proteomics starts with lysis, followed by in-solution digestion. Lysis buffers commonly used include detergents and other reagents for efficient solubility of the proteins. However these reagents are, for the most part, incompatible with downstream analytica...
ORGANISM(S): Homo sapiens (Human) 
2019-02-28 | PXD011665 | Pride
Q eaxctive hf Lysis Proteomics Hela S-trap 
ProteomicsAn Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation
The performance of the S-Trap protocol for phosphoproteomics studies for proteome and phosphoproteome was systematically evaluated in big-scale and small-scale samples. The results showed the existing protocol was problematic for phsophopeptides enrichment. After employing optimized S-Trap methodol...
ORGANISM(S): Homo sapiens (Human) 
2023-06-19 | PXD037751 | Pride
Phosphoproteomics  sample preparation  method optimization 
UnknownOptimized Suspension Trapping Method for Phosphoproteomics Sample Preparation.
Not available
S-EPMC10308333 | biostudies-literature
ProteomicsSuspension TRAPping filter (sTRAP) sample preparation for quantitative proteomics in the low µg input range using a plasmid DNA micro-spin column: analysis of the hippocampus from the 5xFAD Alzheimer’s Disease mouse model
Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics study. Here, we demonstrated the use of a low-cost Plasmid DNA spin column for sTRAP. We first evaluated, the reproducibility of this protocol and the low CoV using 4 replicates of 5-10 µg of a synapse enric...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-23 | PXD041045 | Pride
Alzheimer’s disease Proteomics 5xfad mouse model Micro-spin column Strap 
UnknownCoupling suspension trapping-based sample preparation and data-independent acquisition mass spectrometry for sensitive exosomal proteomic analysis.
Not available
S-EPMC9101639 | biostudies-literature
ProteomicsAn ultrafast, reliable and economical approach for quantitative salivary proteomic profiling and oral cancer biomarker discovery
Clinical biomarkers identified by shotgun proteomics require proteins in body fluids or tissues to be enzymatically digested before being separated and sequenced by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). How well peptide signals can be resolved and detected is largely dependent o...
ORGANISM(S): Homo sapiens (Human) 
2019-03-12 | PXD012436 | Pride
Strap Saliva Suspension trapping Hela Clinical proteomics Oral squamous cell carcinoma Fasp Biomarker Oscc 
ProteomicsS-Trap is an ultrafast sample preparation approach for shotgun proteomics
The success of shotgun proteomic analysis depends largely on how samples are prepared. Current approaches such as gel-, solution- or filter-based, although being extensively employed in the field, are time-consuming and less effective with respect to the repetitive sample processing, recovery, and o...
ORGANISM(S): Escherichia coli 
2019-03-28 | PXD010237 | Pride
Proteomics  sample preparation  fasp  suspension trapping  klebsiella pneumoniae  sputum  tuberculosis. 
ProteomicsProteome Dataset for Adult-derived Mouse Microglia
We report methods development for quantitative proteomic analysis of samples with limited number of cells. The improvements include suspension trapping (S-Trap) for sample processing, longer analytical column length (75cm), and a segmented data dependent acquisition approach. The modified approach w...
ORGANISM(S): Mus musculus (Mouse) 
2019-04-18 | PXD012006 | Pride
Neuroinflammation Img cells Proteomics Mass spectrometry Microglia Dataset brief Glia 
UnknownCell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of Cell Mechanics.
Not available
S-EPMC4897707 | biostudies-literature
Sort   by:  
 Page size