Sort   by:  
 Page size 
ProteomicsAn ultra-high-resolution IonStar strategy enhancing accuracy and precision of MS1-based proteomics and an extensive comparison with state-of-the-art SWATH-MS in large-cohort quantification
Quantitative proteomics in large cohorts is highly valuable for biomedical/pharmaceutical investigations but often suffers from suboptimal reliability, accuracy, and reproducibility. Here we describe a new ultra-high-resolution (UHR) IonStar method achieving precise/reproducible protein measurement ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2021-06-24 | PXD017812 | Pride
Ultrahigh resolution ionstar Swath-ms 120k technical replicates 
ProteomicsMembrane enriched temperature series proteome of Francisella tularensis subspecies novicida in wild type and lipid A acyltransferase null mutants.
Quantitative proteomics has matured into an established tool and longitudinal proteomic experiments have begun to emerge. However, no effective, simple-to-use differential expression method for longitudinal proteomics data has been released. Typically, such data is noisy, contains missing values, ha...
ORGANISM(S): Francisella tularensis subsp. novicida (strain U112) 
2023-01-24 | PXD025439 | Pride
Francisella tularensis subspecies novicida Lpxd1 Lipid a Knockout Lpxd2 Francisella novicida Orbitrap elite Temperature series Lpxl Francisella tularensis 
ProteomicsImmunopeptidome of HeLa-CIITA cells silenced for TAX1BP1(T6BP) expression
CD4+ T lymphocytes play a major role in the establishment and maintenance of immunity. They are activated by antigenic peptides derived from extracellular or newly synthesized (endogenous) proteins presented on the surface of antigen presenting cells (APCs) by the MHC-II molecules. The pathways lead...
ORGANISM(S): Homo sapiens (Human) 
2022-09-22 | PXD024417 | Pride
Human Lc-msms Autophagy receptor 
ProteomicsGlobal mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using 2C_2
S. aureus strain JKD6009 RNase III-HTF and USA300 RNase III-HTF were used in our study to capture the RNA-binding proteome in Staphylococcus aureus. Both strains were inoculated in TSB (Tryptone soya broth, Oxioid CM0129) and overnight cultured in 37°C, 200 rpm shaker. Strain JKD6009 RNase III-HTF w...
ORGANISM(S): Staphylococcus aureus 
2022-05-31 | PXD023427 | Pride
Silica purification Uv cross-linking Rbdome Protein-rna interactions Rna-binding proteins Rbpome Staphylococcus aureus 
ProteomicsGlobal mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using 2C_1
S. aureus strain JKD6009 RNase III-HTF and USA300 RNase III-HTF were used in our study to capture the RNA-binding proteome in Staphylococcus aureus. Both strains were inoculated in TSB (Tryptone soya broth, Oxioid CM0129) and overnight cultured in 37°C, 200 rpm shaker. Strain JKD6009 RNase III-HTF w...
ORGANISM(S): Staphylococcus aureus 
2022-05-31 | PXD023368 | Pride
Silica purification Uv cross-linking Rbdome Protein-rna interactions Rna-binding proteins Rbpome Staphylococcus aureus 
ProteomicsGlobal mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using 2C_3
S. aureus strain JKD6009 RNase III-HTF and USA300 RNase III-HTF were used in our study to capture the RNA-binding proteome in Staphylococcus aureus. Both strains were inoculated in TSB (Tryptone soya broth, Oxioid CM0129) and overnight cultured in 37°C, 200 rpm shaker. Strain JKD6009 RNase III-HTF w...
ORGANISM(S): Staphylococcus aureus 
2022-05-31 | PXD023408 | Pride
Silica purification Uv cross-linking Rbdome Protein-rna interactions Rna-binding proteins Rbpome Staphylococcus aureus 
ProteomicsOptimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis
Label-free quantitative proteomics was employed to compare the protein content of extracellular vesicles isolated by various differential centrifugation-based approaches from expressed prostatic secretions in urine (EPS-urine) from men with prostate cancer. The developed optimized approach improved ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-09 | PXD026974 | Pride
Human Ev Label-free Extracellular vesicles Expressed prostatic secretions in urine Urine Eps-urine Prostate cancer Lc-ms/ms 
ProteomicsDynamic transcriptome-proteome correlation networks reveal human myeloid differentiation and neutrophil-specific programming
Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear bloo...
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD013785 | Pride
Transcriptomics Proteomics Neutriphils Myeloid differentiation 
ProteomicsThe tumor suppressor Fhit in complex with the alarmone Ap3A binds to the ribosome and impedes translation
Upon cellular stress, the concentration of the alarmone diadenosine triphosphate (Ap3A) increases and is thought to trigger downstream adaptive processes. The known Ap3A-hydrolase fragile histidine triad (Fhit) is known to form a complex with Ap3A. The molecular mechanism of Fhit-Ap3A signaling is h...
ORGANISM(S): Homo sapiens (Human) 
2022-12-20 | PXD029645 | Pride
Ap-ms Mass spectrometry Ribosome Xl-ms Fhit Ap3a Translation 
ProteomicsMonosomes actively translate synaptic mRNAs in neuronal processes
In order to deal with their huge volume and complex morphology, neurons localize mRNAs and ribosomes near synapses to produce proteins locally. A relative paucity of polyribosomes (considered the active sites of translation) detected in electron micrographs of neuronal processes (axons and dendrites...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-01-27 | PXD016552 | Pride
Neuronal processes Monosomes Mrna Protein synthesis 
Sort   by:  
 Page size