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UnknownDevelopment of substrate-selective probes for affinity pulldown of histone demethylases.
Not available
S-EPMC4301071 | biostudies-literature
GenomicsReciprocal interactions of human C10orf12 and C17orf96 with PRC2 revealed by BioTAP-XL crosslinking and affinity purification
Short-read sequencing of DNA fractions of the BioTAP-XL pull-downs on human EZH2 and interacting proteins. ChIP-seq-like pulldown/input samples
ORGANISM(S): Homo sapiens 
2014-02-01 | E-GEOD-53495 | biostudies-arrayexpress
ProteomicsWASH pulldown proteomics analysis
Affinity pulldown-paired quantitative proteomic analysis of HA-YFP-NLS.WASH interactors.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026512 | Pride
293t Wash Ip-ms 
ProteomicsProximity labelling and cryomill affinity purification compared for Trypanosoma brucei proteins with unconfined, semi-confined and confined localisation
Identification of protein-protein interactions is a major contributor for understanding protein function and elucidating molecular and cellular mechanisms. Two strategies are currently popular for identification of protein interaction partners directly from the cellular environment. Affinity purific...
ORGANISM(S): Trypanosoma brucei 
2022-11-30 | PXD031245 | Pride
Cryomilling Affinity purification Proteome Bioid Interactome 
UnknownA model of pulldown alignments from SssI-treated DNA improves DNA methylation prediction.
Not available
S-EPMC6700779 | biostudies-literature
ProteomicsRNA pulldown-MS, Dlg4 RNA in mouse brain
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034159 | Pride
Pulldown M6a Rna Lc-ms/ms 
UnknownAffinity pulldown of γ-secretase and associated proteins from human and rat brain.
Not available
S-EPMC4373488 | biostudies-literature
ProteomicsTeamwork of clustered low affinity κB sites and accessory factors regulates transcriptional strength of NF-κB RelA dimers
Non-consensus binding sites of transcription factors are often observed within the promoters and enhancers of various genes; however, their effect on transcriptional strength is unclear. Within the promoters and enhancers of NF-κB-responsive genes, we identified clusters of non- consensus κB DNA sit...
ORGANISM(S): Mus musculus (Mouse) 
2025-10-13 | PXD065004 | Pride
Transcription Cofactor Promoter Κb dna Nf-kappa b (nf-κb) 
ProteomicsRNA pulldown-MS, Dlg4 RNA in mHippoE-2 cells
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034161 | Pride
Pulldown M6a Rna Lc-ms/ms 
ProteomicsA peptidisc-based AP/MS approach to explore the interactome of the bacterial Sec translocon
We recently presented the peptidisc membrane mimetic for the global reconstitution of membrane proteomes into water-soluble detergent-free particles, termed peptidisc library. We here develop a method that combines the peptidisc library with affinity pulldown and mass spectrometry (AP/MS) to explore...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) 
2023-03-22 | PXD018866 | Pride
Mass spectrometry Affinity tagging Sec translocon Protein translocation Peptidisc Protein complex analysis Membrane mimetics Membrane protein complexes Protein-protein interactions Affinity purification/mass spectrometry (ap/ms) 
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