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ProteomicsHeLa cell protein interactome of polyamine affinity-based probes.
HeLa cells were incubated with affinity-based probes designed as synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine, while a monoamine compound served as a control. Probe-protein interactions were captured in situ using UV-induced crosslinking. After cell lysis, biorth...
ORGANISM(S): Homo sapiens (Human) 
2025-09-22 | PXD058706 | Pride
Hela Affinity-based probes Polyamines Putrescine Spermidine Protein-metabolite interactions Spermine 
ProteomicsShotgun analysis of proteins in HeLa cells following treatment with polyamine affinity-based probes
HeLa cells were incubated with affinity-based probes, synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine. Cells were photoirradiated briefly and proteins were extracted, digested with trypsin, and the resulting peptides were labeled with TMT. Proteins were quantified a...
ORGANISM(S): Homo sapiens (Human) 
2025-09-22 | PXD062452 | Pride
Hela Total protein levels Polyamines Affinity-based probes 
ProteomicsOlaparib based photo-affinity probes for PARP-1 detection in living cells
The poly-ADP-ribose polymerase (PARP) is a protein from the family of ADP-ribosyltransferases that catalyzes poly adenosine diphosphate ribose (ADPR) formation in order to attract the DNA repair machinery to DNA damage sites. Inhibition of PARP activity by olaparib can cause cell death which is of c...
ORGANISM(S): Homo sapiens (Human) 
2020-04-29 | PXD018661 | Pride
Olaparib  parp-1  bioorthogonal chemistry  chemical proteomics  photoaffinity probes 
ProteomicsHeLa cell protein interactome of polyamine affinity-based probes (probe-modified peptides)
HeLa cells were incubated with affinity-based probes designed as synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine. Probe-protein interactions were captured in situ using UV-induced crosslinking. After cell lysis, the probe–protein conjugates were subjected to bioorth...
ORGANISM(S): Homo sapiens (Human) 
2025-09-22 | PXD066655 | Pride
Photoaffinity probes Polyamines Probe-modified peptides 
UnknownAffinity-based covalent sialyltransferase probes enabled by ligand-directed chemistry.
Not available
S-EPMC11749336 | biostudies-literature
UnknownAffinity probes based on small-molecule inhibitors for tumor imaging.
Not available
S-EPMC9647038 | biostudies-literature
ProteomicsXanthohumol Activity-Based Probes Targeting Flavonoid Catabolism
Xanthohumol, the principle prenylflavonoid found in hops (Humulus lupulus), is a potential anti-inflammatory agent in the gut. To investigate the interactions between xanthohumol and catabolic gut bacteria, and its role in the anti-inflammatory pathway, a suite of xanthohumol-derived activity-based ...
ORGANISM(S): Eubacterium Ramulus (ncbitaxon:39490) Escherichia Coli (ncbitaxon:562) 
2022-02-04 | MSV000088789 | MassIVE
ProteomicsMapping of mycobacterial lipolytic enzymes involved in triacylglycerol accumulation as intrabacterial lipid inclusions using multi-target inhibitor-like affinity-based probes
Mycobacteria are known for their unique and complex cell wall architecture, characterized by distinct lipid-rich outer layers, making lipid metabolism a central point of their physiological processes. One crucial aspect of all mycobacteria is their ability to metabolize host lipids and to accumulate...
ORGANISM(S): Mycobacterium abscessus 
2025-05-12 | PXD048770 | Pride
Mycobacterium Lc_msms Activity-based protein profiling  multitarget inhibitor-like affinity-based probes  lipid metabolism  cyclipostins & cyclophostin  oxadiazolone 
UnknownAffinity-based probes based on type II kinase inhibitors.
Not available
S-EPMC3523701 | biostudies-literature
ProteomicsActivity-based protein profiling in human M2 macrophages using DHA-based photoaffinity probes
17-Hydroxydocosahexaenoic acid (17-HDHA), an oxidative metabolite of the fish oil constituent docosahexaenoic acid (DHA, 22:6 n-3), is a signaling lipid with anti-inflammatory properties. The molecular mechanisms underlying the biological effect of 17-HDHA are poorly understood. Here, we report the ...
ORGANISM(S): Homo sapiens (Human) 
2023-04-28 | PXD027600 | Pride
Human Abpp Photoaffinity Macrophage 
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