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ProteomicsRapid factor depletion highlights intricacies of nucleoplasmic RNA degradation
Turnover of nucleoplasmic transcripts by the multi-subunit RNA exosome is mediated by two adaptors; the Nuclear EXosome Targeting (NEXT) complex and the Poly(A) tail eXosome Targeting (PAXT) connection. Functional analyses of NEXT and PAXT have largely been based on long-term factor depletion strate...
ORGANISM(S): Homo sapiens (Human) 
2021-12-23 | PXD026774 | Pride
Rna exosome Tmtpro Next Paxt Degron tag 
ProteomicsA functional connection between the Microprocessor and a variant NEXT complex
In mammalian cells, primary miRNAs are cleaved at their hairpin structures by the Microprocessor complex, whose core is composed of DROSHA and DGCR8. Here, we show that 5’flanking regions, resulting from Microprocessor cleavage, are targeted by the RNA exosome in mouse embryonic stem cells (mESCs). ...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-22 | PXD053925 | Pride
Zcchc8-dgcr8 interaction Rna structure Microprocessor Nuclear rna decay The next complex 
ProteomicsDual agonistic and antagonistic roles of ZC3H18 provides for co-activation of distinct nuclear RNA decay pathways
The RNA exosome is a versatile ribonuclease. In the nucleoplasm of mammalian cells, it is assisted by its adaptors the Nuclear EXosome Targeting (NEXT) complex and the PolyA eXosome Targeting (PAXT) connection. Via its association with the ARS2 and ZC3H18 proteins, NEXT/exosome is recruited to cappe...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD045842 | Pride
Ars2 Nuclear rna decay Next Paxt Activation Zc3h18 Inhibition 
ProteomicsBiochemically validated structural model of the 15-subunit intraflagellar transport complex IFT-B
Cilia are ubiquitous eukaryotic organelles impotant for cellular motility, signaling, and sensory reception. Cilium formation requires intraflagellar transport of structural and signaling components and involves 22 different proteins organized into intraflagellar transport (IFT) complexes IFT-A and ...
ORGANISM(S): Homo sapiens (Human) 
2022-12-02 | PXD037049 | Pride
Protein structure Intraflagellar transport Cross-linking Proteomics Cilia 
ProteomicsBreast Cancer
Endocrine resistance in breast cancer is a major clinical problem with poorly understood mechanisms. Mass spectrometry-based proteomics of a clinically-relevant tamoxifen-resistant cell line model identified increased levels of minichromosome maintenance proteins (MCM), including MCM3, as central in...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD001087 | Pride
Ndrg1 Tamoxifen resistance Estrogen receptor-positive breast cancer Cell cycle Minichromosome maintenance proteins (mcm) Dna replication 
ProteomicsAn extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells
Recruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By charact...
ORGANISM(S): Homo Sapiens (human) 
2019-10-31 | PXD016070 | JPOST Repository
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