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ProteomicsThe Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast function
Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-09-09 | PXD022584 | Pride
Arabidopsis Not4a e3 ligase Pgr3 expression 
ProteomicsMass spectrometry based quantitative proteome analyses for the import mutants ppi2 and tic56-1 as well as for spectinomycin treated Arabidopsis thalian plants
We acquired quantitative proteomics data from eight week old ppi2 and tic56-1 mutants as well as from wild type treated with 60 µg/ml spectinomycin and related them to their respective wildtype reference. Protein identification and quantification was performed by data-independent MSE acquisition on ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-14 | PXD004125 | Pride
Hdmse Tic56-1 Plastid Spectinomycin Plastidic translation Quantitative mass spectrometry Shotgun proteomics Toc-tic-import machinery Hi3 quantification Ppi2 
ProteomicsMild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts
The ppi2 mutant introgressed into the Columbia-0 ecotype as previously reported. Doublemutant ppi2xrpn8a was crossed with the above referenced genotypes using heterozygous ppi2 plants. After 2 days of stratification at 4 °C the plants were grown on half-strength Murashige and Skoog (M&S) medium supp...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-27 | PXD017126 | Pride
Arabidopsis Chafradic N-termiomics 
ProteomicsProteomic data of MG132 treated A. thaliana WT and ppi2
A mutation in the proteasomal lid subunit Rpn8a alters proteasome composition and exerts a beneficial effect on photosynthesis. The effect is particularly visible in a double mutant between rpn8a and the plastid protein import receptor Toc159 (ppi2xrpn8a) but also in an rpn8a single mutant that show...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-27 | PXD014560 | Pride
Ubiquitin proteasome system  plastid protein import 
ProteomicsProteomic data of A. thaliana WT vs. rpn8a, sp1 and ppi2 vs.sp1xppi2, rpn8axppi2
A mutation in the proteasomal lid subunit Rpn8a alters proteasome composition and exerts a beneficial effect on photosynthesis. The effect is particularly visible in a double mutant between rpn8a and the plastid protein import receptor Toc159 (ppi2xrpn8a) but also in an rpn8a single mutant that show...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-27 | PXD014531 | Pride
Ubiquitin proteasome system  plastid protein import 
ProteomicsThe zinc repository of Cupriavidus metallidurans
Zinc is a central player in the metalloproteomes of prokaryotes and eukaryotes. We used a top-down quantitative proteomic approach to reveal the repository of the zinc pools in the proteobacterium Cupriavidus metallidurans. About 60% of the theoretical proteome of C. metallidurans were identified, q...
ORGANISM(S): Cupriavidus metallidurans 
2016-05-04 | PXD001356 | Pride
Zinc cupriavidus 
ProteomicsIdentification of protein N-termini in Cyanophora paradoxa cyanelles: Transit peptide composition and sequence determinants for precursor maturation
Glaucophytes, rhodophyta and chloroplastida represent the three evolutionary lineages that diverged after primary endosymbiosis. Comparative analyses between members of these three lineages are a rich source of information on ancestral plastid features. We analyzed the composition and the cleavage s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-14 | PXD002187 | Pride
Cyanophera paradoxa Ms Chloroplast transit peptide Tails 
ProteomicsThe chloroplast outer and inner envelope membrane translocase (TOC/TIC) interaction network identifies a 23 kDa glycine-rich protein as new subunit of the 1-MDa TIC complex
The protein import machinery is essential for chloroplast biogenesis, but knowledge on its exact functioning and the subunits involved in protein translocation is incomplete. Using TAP-tagged Toc159, we reproducibly identified a translocase interaction network that comprises in addition to known TOC...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-02-22 | PXD008469 | Pride
Arabidopsis thaliana Tic 
ProteomicsThe proteome of tic56-1 plastids after thermolysin treatment
Analysing of the proteome of tic56-1 (tic56-1-plus) and wild type (wt-plus) plastids after thermolysin treatment as well as the proteome of non-thermolysin treated wild type plastids (wt-minus) and comparison of the proteome data sets.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-02-03 | PXD001207 | Pride
Tic56 Protease protection assay Chloroplast Thermolysin 
ProteomicsTAILS with Arabidopsis thaliana wilde typ, tic56-1and ppi2
Aim was to analyse the in vivo start position of proteins in the import-mutant plant lines tic56-1 and ppi2 in contrast to wild type. Therefor we enriched N-terminal peptides originating from total protein of the three plant lines and detect them by mass spectrometry. This method is called TAILS and...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-02-03 | PXD000660 | Pride
Tic56-1 Plastidic import Tails Ppi2 
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