Proximity labelling using transient expression of the same exogenous BirA*-RAB18 construct in wild-type HeLa cells and in otherwise isogenic RAB3GAP1-, RAB3GAP2- and TRAPPC9-null HeLa cell lines.
MOB1 is a conserved protein that regulates cellular proliferation versus apoptosis, centrosome duplication and cellular differentiation in multicellular eukaryotes and also cytokinesis and division axis orientation in unicellular and multicellular eukaryotes. Toxoplasma gondii, an obligate intracell...
The formation of plasma membrane microdomains, known as lipid rafts, is driven by specific interactions among cholesterol, sphingolipids, and certain proteins such as flotillins. Flotillins are known to oligomerize and associate with defined lipid assemblies, thereby promoting the coalescence and st...
Hepatocyte nuclear factor 4 (HNF4) is a transcription factor that acts as a master regulator of genes in several endodermal-derived tissues, including the intestine in which it plays a central role during development and tumorigenesis. To better delineate the mechanisms by which HNF4 can interfere d...
This project uses an MCF10A clone (B2B1) expressing chimeric synthetic receptors for ErbB1 (EGFR) and ErbB2 (HER2) along with BirA*-fused inducible alleles of CSE1L or NUP37. Cells were cultured with reconstituted basement membrane, BirA* alleles induced with doxycycline, ErbB receptors dimerized w...
Protein-protein proximity of core pluripotency transcription factors plays an important role during cell reprogramming. Pluripotent embryonic stem (ES) cell identity is controlled by a trio of transcription factors: Sox2, Oct4, and Nanog. These proteins often bind to closely localized genomic sites....
Purpose of these experiments was to assess the effect of glucocorticoid receptor SUMOylation on its protein-protein interactions using BioID. Experiments were performed with SUMOylation deficient GR3KR (K277,293,703R) fused to the BirA* biotin ligase tag.
