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ProteomicsM. bovis BCG PknG physiological substrates
Mycobacterial Ser/Thr kinases play a critical role in bacterial physiology and pathogenesis. Linking kinases to the substrates they phosphorylate in vivo, thereby elucidating their exact functions, is still a challenge. The aim of this work was to associate protein phosphorylation in mycobacteria wi...
ORGANISM(S): Mycobacterium bovis BCG str. Pasteur 1173P2 
2018-03-23 | PXD006473 | Pride
Kinases Pkng  phosphoproteomics  mycobacteria 
ProteomicsComparison of Cell Wall Proteomics of Drug Resistant M. smegmatis with and without Sub-inhibitory Rifampicin Exposure
We previously showed sub-lethal exposure to the front-line anti-TB drug rifampicin resulted in substantial adaptive remodelling of the proteome of the model organism M. smegmatis in the drug sensitive mc2155 strain (WT). Here we investigate whether these responses are conserved in an engineered, iso...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2021-11-02 | PXD027855 | Pride
Abc transporters Rifampicin Proteomics Mass spectrometry Mycobacterium smegmatis Phenotypic adaption 
ProteomicsQuantitative proteomic analysis of HIV-1 Tat-induced dysregulation in SH-SY5Y neuroblastoma cells
Despite affecting up to 70% of HIV+ patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HAND are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on HIV-Tat treated SH-SY5Y neurob...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-18 | MSV000083590 | MassIVE
ProteomicsProteomic Time-Course Investigation into the Effects of Exposure of Mycobacterium smegmatis to Sub-Lethal Dose of Rifampicin
Drug susceptible Mycobacterium smegmatis (Mc2-155) was exposed to a sub-MIC concentration of antimycobacterial drug Rifampicin (treated) or DMSO (control). Cells were harvested at 30, 255 and 300 minutes post-exposure and the proteome at each time analysed using LC-MS/MS on a Thermo Q-Exactive.
ORGANISM(S): Mycobacterium Smegmatis Str. Mc2 155 (ncbitaxon:246196) 
2019-03-18 | MSV000083589 | MassIVE
ProteomicsCell wall enrichment unveils proteomic changes in the cell wall during treatment of Mycobacterium smegmatis with sub-lethal concentrations of Rifampicin
In this study, we successfully developed two gel-free approaches to specifically look at cell wall proteins in Mycobacterium smegmatis. The cell wall was subjected to differential centrifugation, differential detergent solubilisation and phase separation to yield the genuine cell wall proteome. The ...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2019-04-09 | PXD008075 | Pride
Cell wall proteomics Mycobacteria Mass spectrometry Rifampicin Tb drugs Tb Sub lethal 
ProteomicsPhosphoproteomics reveals alternative roles of PknG in mycobacterial pathogenesis
Pathogenic mycobacteria, such as Mycobacterium tuberculosis, modulate the host immune system to evade clearance and promote long-term persistence, resulting in disease progression or latent infection. Understanding the mechanisms pathogenic mycobacteria use to evade the host immune system is critica...
ORGANISM(S): Mus musculus (Mouse) Mycobacterium tuberculosis 
2025-11-17 | PXD031055 | Pride
Phosphoproteomics Mycobacterium tuberculosis Substrate identification Serine/threonine protein kinases Post-translational modifications Mass spectrometry Phosphorylation 
ProteomicsProteogenomic analysis of Mycobacterium smegmatis using high resolution mass spectrometry
Biochemical evidence is vital for accurate genome annotation. The integration of experimental data collected at the proteome level using high resolution mass spectrometry allows for improvements in genome annotation by providing evidence for novel gene models, while validating or modifying others. ...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2016-03-31 | PXD003500 | Pride
Mass spectrometry Genome annotation Mycobacterium smegmatis Proteogenomics Proteomics 
ProteomicsAssociating H2O2 and NO related changes in the proteome of Mycobacterium smegmatis with enhanced survival in macrophages
In this study, we used mass spectrometry-based discovery proteomic analysis to examine the cell-wide changes of Mycobacterium smegmatis, in response to sub-lethal concentrations of H2O2 and nitric oxide (NO) over time. Preliminary results have shown that sub-lethal concentrations of reactive oxyge...
ORGANISM(S): Mycobacterium smegmatis 
2019-11-12 | PXD010020 | Pride
No Sub-lethal concentrations Signalling Ros H2o2 Mycobacterium Proteomics 
ProteomicsHIV-Tat treatment of human neurons in vitro over time: towards a model for the molecular pathology of HIV-associated neurocognitive disorders (~70% cell culture confluence dataset)
We have used LC-MSMS discovery proteomics to identify the quantitative, cell-wide changes that occur when non-transformed, differentiated human neurons are treated with HIV-Tat over time. This may represent a novel cell culture model for initial HIV-associated neurocognitive disorder progression.
ORGANISM(S): Homo sapiens (Human) 
2017-05-19 | PXD006332 | Pride
Hiv-tat Hiv associated neurocognitive disorders Human neuronal cells Lcmsms 
ProteomicsHIV-Tat treatment of human neurons in vitro over time: towards a model for the molecular pathology of HIV-associated neurocognitive disorders (~90% cell culture confluence dataset)
We have used LC-MSMS discovery proteomics to identify the quantitative, cell-wide changes that occur when non-transformed, differentiated human neurons are treated with HIV-Tat over time. This may represent a novel cell culture model for initial HIV-associated neurocognitive disorder progression.
ORGANISM(S): Homo sapiens (Human) 
2017-05-19 | PXD006314 | Pride
Hiv-tat Hiv associated neurocognitive disorders Human neuronal cells Lcmsms 
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