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ProteomicsA new method for differential proteomic analysis utilizing native MS-electropherograms: a comparison between plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS
MS identification has long been used for PAGE-separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole-gel slicing and quantitative LC-MS/MS aiming c...
ORGANISM(S): Homo sapiens (Human) 
2017-09-08 | PXD006773 | Pride
Gel slicing Quantitative lc-ms/ms Native ms-electropherogram Native 1d-page Human plasma and serum Differential proteomic analysis 
ProteomicsBlue Native PAGE co-fractionation of purified human endosomes
EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified ...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054684 | Pride
Co-fractionation Human Endosomes Bn-page Endomap 
ProteomicsGlycine receptor complex analysis using immunoprecipitation-blue native gel electrophoresis-mass spectrometry
The pentameric glycine receptor (GlyR), comprising the α1 and β subunits, is a major inhibitory ionotropic receptor in brainstem and spinal cord. GlyRs interact with gephyrin (GPHN), a scaffold protein that anchors the GlyR in the plasma membrane and enables it to form clusters in glycinergic postsy...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-12 | PXD017151 | Pride
Blue native gel electrophoresis Proteomics Protein complex Glycine receptor Iqsec3 
ProteomicsBN-PAGE analysis of DJ-1 KO dopaminergic neurons
We subjected digitonin-soluble lysates of DJ-1 KO and isogenic control neurons to non-denaturing blue-native PAGE (BN-PAGE) to visualize and separate multi-subunit protein complexes associated with DJ-1.
ORGANISM(S): Homo sapiens (Human) 
2025-12-23 | PXD069553 | Pride
Dopaminergic neurons Dj-1 Bn-page 
UnknownQualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE.
Not available
S-EPMC8895881 | biostudies-literature
ProteomicsBrain region differences in α1 and α5 subunit-containing GABAA receptor proteome revealed by affinity purification and Blue Native PAGE proteomics
GABAA receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β and one γ or δ subunit. From the six α subunit genes, the α5 subunit displays a limited spatial expression pattern and is known to mediate both phasic and tonic...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD047550 | Pride
Gabaar Proteomics Mass spectrometry Immunoprecipitation 
ProteomicsShotgun Proteomics of BN-PAGE-Resolved Chloroplasts
Blue Native-PAGE resolves protein complexes in their native state. When coupled with immunoblotting, it can be used to identify the presence of high molecular weight complexes at high resolution for any protein, given a suitable antibody. To identify proteins in high molecular weight complexes on a ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-12-23 | PXD003162 | Pride
Oligomeric state Native gel Protein complex Native molecular weight Chloroplast 
ProteomicsBlue Native Page based separation of the TNFRSC
Separation and relative quantification by targeted mass spectrometry of the TNFRSC members pior and after treatment with a generic deubiquitinating enzyme
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD019847 | Pride
Bn page tnfrsc prm 
ProteomicsCross-linked ammonia monooxygenase complex in BN-PAGE gel from Nitrososphaera viennensis
A band containing the ammonia monooxygenase complex from Nitrosospheara viennensis was cut from a Blue Native PAGE gel and cross linked using DSSO.
ORGANISM(S): Nitrososphaera viennensis EN76 
2023-02-13 | PXD034475 | Pride
Ammonia oxidizing archaea Ammonia monooxygenase Nitrification Copper membrane bound monooxygenase 
ProteomicsQuantitative erythrocyte membrane proteome analysis with Blue-Native SDS PAGE
Not available
ORGANISM(S): Homo sapiens (Human) 
2011-04-15 | PRD000092 | Pride
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