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ProteomicsRAS-p110α signalling pathway in macrophages and its impact on both the initiation and resolution of inflammation
This study presents a secretome analysis of bone marrow-derived macrophages (BMDMs) from wild-type (WT) and RAS-p110α-disrupted macrophages. Samples were collected under both unstimulated conditions and during phagocytosis of apoptotic cells. The aim was to identify differential regulatory factors s...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-28 | PXD057794 | Pride
Proteomics Inflammation Macrophages Ras-pi3k Lc-ms/ms 
ProteomicsKinase activity ranking (KAR) quantifies the contribution of protein kinases to the regulation of cell viability
New method to determine kinase activity based on phosphoproteomics data
ORGANISM(S): Homo sapiens (Human) 
2017-07-06 | PXD005073 | Pride
Aml kinase phosphorylation 
ProteomicsCell rearrangement during angiogenesis requires suppression of actomyosin at cell-cell contacts via PI3-kinase.
Sprouting angiogenesis is a highly dynamic process which relies on the continuous interchange of endothelial cell relative position within the vascular sprout, a process mediated by cell rearrangements. Here we take advantage of a previous identified role of p110a/PI3K regulating endothelial cell mo...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-02 | PXD007060 | Pride
Mascot Pescal Phosphoproteomics Qexactive-plus Mice 
ProteomicsKinase Substrate Enrichment Analysis Provides Insights into the Heterogeneity of Signaling Pathway Activation in Leukemia Cells
Experiments were performed in AML (Acute Myeloid Leukemia) cell lines and in primary cells coming from patients with AML and healthy donors. The AML cell lines P31/Fuj and Kasumi-1 were treated with vehicle (DMSO), 100 nM or 1000 nM of the PI3K/mTOR inhibitor AZ123 or the mTOR inhibitor Ku0063794 f...
ORGANISM(S): Homo sapiens (Human) 
2013-04-09 | PXD000185 | Pride
Tio2 phosphoenrichment Label free quantification Aml primary cells Pi3k/mtor inhibitors 
ProteomicsEnvironmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines
The breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition ...
ORGANISM(S): Homo sapiens (Human) 
2014-04-11 | PXD000472 | Pride
Label free Mcf7 Room temperature exposition Tio2 phosphoenrichment 
ProteomicsPhosphoproteomics data classify haematological cancer cell lines according to tumour type and sensitivity to kinase inhibitors
Acute myeloid leukemia, lymphoma and multiple myeloma cell lines were grown for 24h in RPMI medium and cells were harvested by centrifugation. Samples were digested with trypsin and subjected to phosphoenrichment using IMAC. Phosphopeptides were run in a LTQ-Orbitrap-XL. Peaks lists were generated ...
ORGANISM(S): Homo sapiens (Human) 
2013-04-26 | PXD000217 | Pride
Kinase inhibitor Drug sensitivity Imac Phosphoproteomics Hierarchical clustering analysis Blood malignancies Acute myeloid leukemia 
ProteomicsAn increase in oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells with acquired resistance to PI3K/mTOR inhibitors
Proteomics and phosphoproteomics analysis of MCF7 cells sensitive and resistant to the PI3K inhibitor GDC-0941. The analysis was done for parental and 3 resistant cell lines maintained in the presence or the absence of the drug. One of the resistant cell lines (G2) was also treated with vehicle or t...
ORGANISM(S): Homo sapiens (Human) 
2018-04-09 | PXD003594 | Pride
Human Phosphoproteomics Metabolism Ltq-orbitrap Tio2 Pi3k resistance Q-exactive plus 
ProteomicsCancer Associated Fibroblast-FAK regulates malignant cell metabolism
Breast and pancreatic cancers are characterised by profound metabolic changes1,2. Until recently, these changes have been ascribed to their intrinsic genetic profiles2-4. However, the contribution of cancer-associated fibroblasts (CAFs) to cell metabolism has not been fully investigated5. Here, we p...
ORGANISM(S): Mus musculus (Mouse) 
2020-02-18 | PXD008276 | Pride
Fak Metabolism Fibroblast 
ProteomicsPhosphoproteomics identifies determinants of PAK inhibitor sensitivity in AML cells
Background The p21 activated kinases (PAK) are frequently dysregulated in cancer and have central roles in oncogenic signalling, prompting the development of PAK inhibitors (PAKi) as anticancer agents. However, such compounds have not reached clinical use because, at least partially, there is a lim...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD056514 | Pride
Acute myeloid leukaemia Phosphoproteomics Machine learning Kinase inhibitors Pak inhibitors Target therapy Pf-3758309 Proteomics Biomarkers Cancer 
ProteomicsTargeted therapy for LIMD1-deficient non-small cell lung cancer subtypes: a Proof-of-Concept synthetic lethal study
An early event in lung oncogenesis is loss of the tumour suppressor gene LIMD1 (LIM domains containing 1); this encodes a scaffold protein, which binds multiple binding partners to suppress tumourigenesis via a number of different mechanisms. Approximately 45% of non-small cell lung cancers (NSCLC) ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD023674 | Pride
Limd1 Tumour suppressor Synthetic lethality Nsclc Targeted therapy Lung cancer 
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