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ProteomicsChemical crosslinking of photosystem I antenna proteins
Chemical crosslinking (DSS) of proteins in conjunction with mass spectrometry was performed to identify protein-protein interactions between the light-harvesting proteins of PSI and PSII.
ORGANISM(S): Chlamydomonas reinhardtii 
2025-04-29 | PXD051493 | Pride
Photosynthesis Lhc Chlamydomonas Light harvesting 
ProteomicsA Cell-Permeable Photosensitizer for Selective Proximity Labeling and Crosslinking of Aggregated Proteome
Intracellular proteome aggregation is a ubiquitous disease hallmark with its composition associated with pathogenicity. Herein, we report on a cell-permeable photosensitizer (P8, Rose Bengal derivative) for selective photo-induced proximity labeling, crosslinking and identification of cellular aggre...
ORGANISM(S): Homo Sapiens 
2023-08-30 | PXD044948 |
ProteomicsIn vitro crosslinking of PAX8 and PRDM3
MS crosslinking of PAX8 and PRDM3 using standard DSSO-based crosslinking
ORGANISM(S): Homo sapiens (Human) 
2021-03-09 | PXD021708 | Pride
Prdm3 Xlinkx Crosslinking-ms Pax8 
ProteomicsA Chemical Tool for Blue Light-Inducible Proximity Photo-crosslinking in Live Cells
We developed a proximity photo-crosslinking method (Spotlight) with a 4-azido-N-ethyl-1,8-naphthalimide (AzNP) moiety that can be converted to reactive aryl nitrene species using ambient blue light-emitting diode light. Using an AzNP-conjugated HaloTag ligand (VL1), blue light-induced photo-crosslin...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD027149 | Pride
Proximity crosslinking / sars-cov-2 / nucleocapsid 
ProteomicsIntegrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints
Integrative structure modelling was applied to understand how auto-phosphorylation of the ABCDE cluster in DNA-PKcs can occur. We used crosslinking restraints to assign sequence to structurally unassigned helices in DNA-PKcs s in the largely disordered region containing the ABCDE cluster. Our final ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-10 | PXD016639 | Pride
Crosslinking-ms Integrative modelling Dna-pkcs 
ProteomicsChemical crosslinking of plastocyanin and the cytochrome b6f complex
In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well ch...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-08-25 | PXD060078 | Pride
Cytochrome b6f Plastocyanin Chlamzdomonas Edc Crosslinking 
UnknownInsights on Chemical Crosslinking Strategies for Proteins.
Not available
S-EPMC9738610 | biostudies-literature
ProteomicsChemical crosslinking and mass spectrometry of the human Rix1 complex
PELP1 is a scaffold protein with diverse cellular functions including its role as the central scaffold for the human Rix1 complex (PELP1, WDR18, TEX10, and SENP3). We have reconstituted the mammalian Rix1 complex and identified a sub-complex made of the conserved Rix1 domain of PELP1 and the WDR18 p...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD037729 | Pride
Q-exactive Lc-ms Rix1 Protein prospector Pelp1 Bs3 Crosslinking 
ProteomicsNative alpha synuclein crosslinking
Alpha synuclein in it's native state in solution was crosslinked using a variety of short-range crosslinking reagents. Crosslinks detected by mass spectrometry were then used as constraints in a discrete molecular dynamics simulation of the synuclein protein, and a structural ensemble of the protein...
ORGANISM(S): Homo sapiens (Human) 
2019-02-28 | PXD011645 | Pride
Alpha synuclein Crosslinking Modelling 
ProteomicsChemical crosslinking of photosynthetic protein complexes in Cyanidioschyzon merolae
Efficient photosynthetic electron transfer relies on transient interactions between photosystem I (PSI) and soluble electron carriers. However, structural information describing these transient interactions are limited in red algae. Here, we applied chemical crosslinking mass spectrometry (XL-MS) to...
ORGANISM(S): Cyanidioschyzon merolae 
2026-08-06 | PXD079525 | Pride
Cyanidioschyzon merolae Photosystem i Fnr Dss Crosslinking 
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