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UnknownRapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood.
Not available
S-EPMC6316042 | biostudies-literature
ProteomicsRapiGest Precipitation Depends on Peptide Concentration
The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. RapiGest consists of a hydrophilic head and a hydrophobic tail, which can be separated by acid hydrolysis. This allows for removal of ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Gallus gallus (Chicken) 
2021-09-07 | PXD025982 | Pride
In-solution digestion Lc-ms Rapigest Detergent Precipitation 
ProteomicsPassive elution of proteoforms from polyacrylamide gels combined with anion exchange purification for in-depth top-down analysis of the human low molecular weight proteome.
We developed a novel sample preparation method by combining Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS (PEPPI-MS) fractionation with Anion-Exchange disc-assisted Sequential sample Preparation (AnExSP) purification, and evaluated its performance in TDP analysis by co...
ORGANISM(S): Homo sapiens (Human) 
2022-09-26 | PXD034353 | Pride
Sds-page Top-down Peppi Anion exchange Stagetip 
ProteomicsEvaluation of Two Simultaneous Metabolomic and Proteomic Extraction Protocols Assessed by Ultra-High-Performance Liq-uid Chromatography Tandem Mass Spectrometry
Untargeted multi-omics analysis of plasma is an emerging tool for the identification of novel biomarkers for evaluating disease prognosis and for a better understanding of molecular mechanisms underlying human disease. The successful application of metabolomic and pro-teomic approaches relies on rep...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD038750 | Pride
Proteomics Reproducibility Multi-omics Metabolomics Plasma 
Proteomicscow MFGM LC-MS/MS - Evaluation of six sample preparation procedures for qualitative and quantitative proteomics analysis of milk fat globule membrane
Pasteurized whole cow milk (Grade A) was purchased from the supermarket. Whole cow milk was centrifuged at 3 000 g at 4°C for 30 min and the fat layer was recovered. The recovered fat was washed with PBS for 20 min at 37°C, and the resuspened mixture was centrifuged again to obtain the milk fat. The...
ORGANISM(S): Bos taurus (Bovine) 
2018-10-19 | PXD009288 | Pride
Mfgm Cow Lc-ms/ms 
ProteomicsTowards a universal sample preparation method for denaturing top-down proteomics of complex proteomes
A universal and standardized sample preparation method becomes vital for denaturing top-down proteomics (dTDP) to advance the scale and accuracy of proteoform delineation in complex biological systems. It needs to have high protein recovery, minimum bias, good reproducibility, and compatibility with...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-22 | PXD018248 | Pride
Histone Ptms Membrane ultrafiltration Chloroform-methanol precipitation Membrane proteins Sp3 Denaturing top-down proteomics Cze-ms/ms Sds Sample preparation 
ProteomicsExploring roles of the chitinase ChiC in modulating Pseudomonas aeruginosa virulence phenotypes
Proteins were precipitated using chloroform and methanol, by the protocol described by Wessel and Flügge (1984). Following precipitation, the proteins were reduced and alkylated before being digested, cleaned and desalted using STrap tips (Zougman A, Selby PJ, Banks RE. 2014). The peptide samples we...
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) Bacteria 
2024-04-30 | PXD048597 | Pride
Glcnac2 Chitinase Pseudomonas aeruginosa Gh18 
ProteomicsMilk bottom-up proteomics: method optimisation.
Milk is a complex fluid whose proteome displays a diverse set of proteins of high abundance such as caseins and medium to low abundance whey proteins such as ß-lactoglobulin, lactoferrin, immunoglobulins, glycoproteins, peptide hormones and enzymes. A sample preparation method that enables high repr...
ORGANISM(S): Bos taurus (Bovine) 
2016-01-26 | PXD002529 | Pride
Replicates. Jersey and holstein cow milk Trypsin digestion Proteome Nlc-esi-ms 
UnknownEvaluation of Two Simultaneous Metabolomic and Proteomic Extraction Protocols Assessed by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry.
Not available
S-EPMC9865896 | biostudies-literature
ProteomicsSoybean Symbiosome Membrane Proteome
Symbiosome membranes (SM) and symbiosome space (SS) fractions were isolated from nitrogen fixing soybean root nodules and analyzed using non-gel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to enrich for hydrophobic integral membrane protein...
ORGANISM(S): Glycine max 
2015-08-12 | PXD001132 | Pride
Soybean Symbiosome Legume Membrane 
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