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To determine the protein partners of ETV6, we expressed ETV6 and HA-tagged ETV6 in Reh pre-B leukemic cells. Anti-HA magnetic beads were used for affinity purification of ETV6-HA (and ETV6 control) from the nuclear and cytoplasmic fraction. Following purification on-beads digest and LC-MS/MS experim...
ORGANISM(S): Homo sapiens (Human) 
2022-01-26 | PXD031001 | Pride
Chromosomal double-strand breaks (DSBs) are resected by 5M-bM-^@M-^Y-nucleases to form 3M-bM-^@M-^Y single-strand DNA (ssDNA) substrates for binding by homologous recombination and DNA damage checkpoint proteins. Two redundant pathways of extensive resection were described both in cells and in vitro...
ORGANISM(S): Saccharomyces cerevisiae 
P388D1 murine macrophages were cultured in 85 mm tissue culture plates to about semi confluency. L. monocytogenes serotypes (1/2a EGD-e, 4a L99, 4b CLIP80459 and 4b F2365) were infected to the P388D1 cell monolayer at a MOI of 100 per eukaryotic cell. Infection was carried for 45 min and followed by...
ORGANISM(S): Listeria monocytogenes 
Alternative Lengthening of Telomeres (ALT) cancer cells are a subset of cancers that depend on the homologous recombination mechanism to extend their telomere length independent of telomerase. ALT cells contain elevated levels of the telomeric-repeat containing long noncoding RNA (TERRA), which is a...
ORGANISM(S): Homo sapiens (Human) 
2022-09-28 | PXD028882 | Pride
Our purpose is to explore the dynamics of a clinically relevant proteome by determining protein turnover, synthesis and breakdown. ]. The alteration of protein turnover represents a new type of biological information which might provide unique insight in disease pathophysiology, e.g. Alzheimer disea...
ORGANISM(S): Homo sapiens (Human) 
2020-01-22 | PXD012462 | Pride
Our purpose is to explore the dynamics of a clinically relevant proteome by determining protein turnover, synthesis and breakdown. ]. The alteration of protein turnover represents a new type of biological information which might provide unique insight in disease pathophysiology, e.g. Alzheimer disea...
ORGANISM(S): Homo sapiens (Human) 
2020-01-22 | PXD012502 | Pride
S. aureus strain JKD6009 RNase III-HTF and USA300 RNase III-HTF were used in our study to capture the RNA-binding proteome in Staphylococcus aureus. Both strains were inoculated in TSB (Tryptone soya broth, Oxioid CM0129) and overnight cultured in 37°C, 200 rpm shaker. Strain JKD6009 RNase III-HTF w...
ORGANISM(S): Staphylococcus aureus 
2022-05-31 | PXD023427 | Pride
S. aureus strain JKD6009 RNase III-HTF and USA300 RNase III-HTF were used in our study to capture the RNA-binding proteome in Staphylococcus aureus. Both strains were inoculated in TSB (Tryptone soya broth, Oxioid CM0129) and overnight cultured in 37°C, 200 rpm shaker. Strain JKD6009 RNase III-HTF w...
ORGANISM(S): Staphylococcus aureus 
2022-05-31 | PXD023368 | Pride
S. aureus strain JKD6009 RNase III-HTF and USA300 RNase III-HTF were used in our study to capture the RNA-binding proteome in Staphylococcus aureus. Both strains were inoculated in TSB (Tryptone soya broth, Oxioid CM0129) and overnight cultured in 37°C, 200 rpm shaker. Strain JKD6009 RNase III-HTF w...
ORGANISM(S): Staphylococcus aureus 
2022-05-31 | PXD023408 | Pride
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