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ProteomicsMembranes prime the RapGEF EPAC1 to transduce cAMP signaling
EPAC1, a cAMP-activated GEF for Rap GTPases, is a major transducer of cAMP signaling in normal cells and a therapeutic target in cardiac diseases. The recent discovery that cAMP is compartmentalized in membrane-proximal nanodomains challenged the current model of EPAC1 activation in the cytosol. Her...
ORGANISM(S): Homo sapiens (Human) 
2023-04-07 | PXD040227 | Pride
Hdxms Epac1 
ProteomicsNucleotide independant as well as ATP-hydrolysis driven steps of Rea1 Linker remodelling drive the removal of assembly factors from pre-ribosomal particles: PhoX cross-linking of Rea1 in presence of ATPgS, AMP-PNP or Apo state.
The ribosome maturation factor Rea1 (or Midasin) catalyses the removal of assembly factors from large ribosomal subunit precursors and to promotes their export from the nucleus to the cytosol. Rea1 consists of nearly 5000 amino-acid residues and belongs to the AAA+ protein family. It consists of a r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-07-29 | PXD053636 | Pride
Cross-linking mass spectrometry Structural biology Structural proteomics 
ProteomicsDevelopment of the eRush system revealed a role for Rab7 in the neosynthetic secretory pathway
Our study proposes the eRush as a powerful tool to further study the secretory pathway and sheds light on Rab7 for its involvement in the neosynthetic transport of the TfR.
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2021-02-03 | PXD010576 | Pride
Membrane traffic  transferrin receptor  tgn exit  vesicular transport  rab gtpase 
ProteomicsA core proliferative program induced by B-cell receptor stimulation in chronic lymphocytic leukemia cells
The B-cell receptor (BCR) signaling is crucial for the pathophysiology of chronic lymphocytic leukemia (CLL). Although the BCR has recently emerged as a new therapeutic target the precise mechanisms by which BCR signaling controls neoplastic B-cell proliferation are still ill characterized. Here, by...
ORGANISM(S): Homo sapiens (Human) 
2021-03-29 | PXD013573 | Pride
Mathematical modelling Machine learning Transcriptome and proteome B-cell receptor (bcr) Temporal response Leukemogenesis Chronic lymphocytic leukemia (cll) Biological network reverse-engineering Cellular program. Lymphoproliferation 
ProteomicsProteomics profiling of Zika- or HIV-infected moncoytes
human primary monocytes purified from 2 healthy blood donors were infected in vitro with Zika virus or HIV for 48 hours. Proteomics profiling of infected versus non infected cells was performed to identify up/down-regulated proteins associated to the infection
ORGANISM(S): Homo sapiens (Human) 
2019-08-22 | PXD014002 | Pride
Monocyte zika 
ProteomicsSecretion of sonic hedgehog - The goal is to identify the proteins implicated in the secretion of sonic hedehog.
The goal is to identify the proteins implicated in the secretion of sonic hedehog.
ORGANISM(S): Homo sapiens (Human) 
2018-07-26 | PXD007990 | Pride
Nano-lc-ms/ms 1d sds-page stacking gel 
ProteomicsA proteomic-informed view of the changes induced by loss of cellular adherence: the example of mouse macrophages
In vertebrates, all cells except circulating blood cells must adhere to support their normal growth and functions. The adherence to extracellular matrix and/or other cells is critical and adherent cells placed in non-adherent conditions either die or form multicellular spheroids. Placing cells in no...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD021593 | Pride
Macrophage Mouse Cellular adherence Lc-ms/ms 
ProteomicsMolecular determinant for MED1 interaction with the VDR-RXR heterodimer
To provide structural insights into the mechanism of the specific association of the coactivator MED1 with the Vitamin D nuclear (VDR)-RXR heterodimer, we used combined structural methods including X-ray crystallography, small angle X-ray scattering (SAXS), NMR, hydrogen-deuterium exchange coupled t...
ORGANISM(S): Homo sapiens (Human) 
2021-02-10 | PXD019530 | Pride
Nuclear receptor Vdr-rxr Xl-ms Hdx-ms 
ProteomicsProMetIS: deep phenotyping of mouse models by proteomics and metabolomics.
We develop new tools enabling high-throughput and combined proteomics and metabolomics data analysis. The tools are validated on two knockout mice models (LAT (Linker For Activation Of T-Cells) and Mx2 KO mice) generated and phenotyped by the PHENOMIN infrastructure.
ORGANISM(S): Mus musculus (Mouse) 
2021-11-18 | PXD028416 | Pride
Mice Liver Lc-ms/ms 
ProteomicsBacillus subtilis RNase M5 cleaves dsRNA in two steps via a two Mg2+-ion dependent mechanism
Here, we present the crystal structure of the catalytic domain of M5 bound to two magnesium ions, which reveals the role of each catalytic site acidic residue in metal binding. We further use hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) to investigate the possibility of structur...
ORGANISM(S): Geobacillus stearothermophilus 
2021-02-10 | PXD019202 | Pride
Rnase Native-ms Hdx-ms 
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