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ProteomicsEvaluation and Optimization of Chemically-Cleavable Linkers for Quantitative Mapping of Small Molecule-Protein Interactomes
Numerous reagents have been developed to enable chemical proteomic analysis of small molecule-protein interactomes. However, the performance of these reagents has not been systematically evaluated and compared. Herein, we report our efforts to conduct a parallel assessment of two widely-used chemica...
ORGANISM(S): Homo sapiens (Human) 
2019-08-21 | PXD014066 | Pride
Azobenzene Dacomitinib Tmt Cleavable biotin linker Dialkoxydiphenylsilane 
ProteomicsProtein composition of catalytically active U7-dependent processing complexes assembled on histone pre-mRNA containing biotin and a photo-cleavable linker
To identify components of the U7 snRNP, we used cleavage-resistant histone pre-mRNA containing biotin and a photo-sensitive linker and purify processing complexes from Drosophila and mouse nuclear extracts.
ORGANISM(S): Mus musculus (Mouse) Drosophila melanogaster (Fruit fly) 
2018-03-08 | PXD008917 | Pride
Flash  lsm11 Histone pre-mrna Photo-cleavable linker 3’ end processing U7 snrnp  slbp 
ProteomicsDevelopment of an sulfoxide-based, CuAAC-enrichable cross-linker
Cross-linking of BSA with a novel cross-linker. Modification of the cross-linker containing peptides with CuAAC-chemistry to attach a cleavable biotin-derivative. Enrichment with streptavidin-beads.
ORGANISM(S): Bos taurus (Bovine) 
2019-10-14 | PXD015080 | Pride
Cuaac Xl-ms Sulfoxide 
UnknownSingle-stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics.
Not available
S-EPMC4078914 | biostudies-literature
UnknownProtein composition of catalytically active U7-dependent processing complexes assembled on histone pre-mRNA containing biotin and a photo-cleavable linker.
Not available
S-EPMC5961079 | biostudies-literature
UnknownSolid-Phase Compatible Silane-Based Cleavable Linker Enables Custom Isobaric Quantitative Chemoproteomics.
Not available
S-EPMC11895830 | biostudies-literature
ProteomicsQuantitative thiol reactivity profiling
Cysteine is unique among all protein-coding amino acids owing to its intrinsically high nucleophilicity. The cysteinyl thiol group can be covalently modified by both a broad range of redox mechanisms and various electrophiles derived from exogenous or endogenous sources. Measuring proteomic cysteine...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-07-24 | PXD016048 | Pride
Thiol Redox Chemical proteomics Electrophile 
ProteomicsDeveloping In Vivo Cross-linking Mass Spectrometry (XL-MS) to Decipher Protein Interaction Landscapes at the Proteome Scale
We have developed a new and robust in vivo cross-linking mass spectrometry (XL-MS) that utilizes a multifunctional MS-cleavable cross-linker to enable the efficient capture, enrichment, and identification of in vivo cross-linked peptides at the whole proteome scale.
ORGANISM(S): Homo sapiens (Human) 
2021-07-30 | PXD012788 | Pride
Human Lc-ms Cross-linking mass spec Ms-cleavable Click chemistry Enrichable Proteome wide 
ProteomicsEnhanced protein-protein interaction network construction promoted by in vivo cross-linking with acid-cleavable click chemistry enrichment
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enables global profiling of protein interactome with direct interaction interfaces in complex biological systems. The alkyne-tagged enrichable cross-linkers are preferred to improve the coverage of low-abu...
ORGANISM(S): Homo Sapiens (human) 
2022-07-14 | PXD035317 | JPOST Repository
ProteomicsStructural surfaceomics of Nomo-1 using DSSO cross-linker with MS3 approach
The project aimed to profile the cell surface proteins of Nomo-1 (AML cell line) using structural surfaceomics for identification of protein conformation-based cancer antigens thereby expanding the toolkit for cancer target discovery for immunotherapeutic targeting. To achieve the goal, cell surface...
ORGANISM(S): Homo sapiens (Human) 
2023-10-04 | PXD035589 | Pride
Ms3 Dsso Xl-ms Cell surfaceome Nomo-1 Aml 
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