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ProteomicsAlterations in immunomodulatory potential of ADSCs under-going osteogenic differentiation in the context of future thera-peutic applications
For proteomic analysis, ADSCs from four independent donors were analyzed (n = 4 biological replicates per condition, OM vs. SM). Cell pellets were lysed in 5% sodium de-oxycholate buffer containing TCEP and CAA, supplemented with protease and phospha-tase inhibitors, followed by sonication and incub...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD073297 | Pride
Osteogenic differentiation Immuno-modulation Proteomics Adipose-derived mesenchymal stem/stromal cells Cell therapy Regenerative medicine 
ProteomicsAllogenic adipose-derived stem cells in DF treatment – are we closer to understanding their activity in humans
OBJECTIVE: To assess the safety and efficacy as well as determination of the molecular mechanism of action of topical allogeneic ADSC therapy in the treatment of ulcers in patients with neuropathic diabetic foot. RESEARCH DESIGN AND METHODS: We examined a total of 57 patients with neuropathic diab...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD032099 | Pride
Human Diabieties Adsc Wound 
ProteomicsNrf2 sequesters Keap1 preventing podosome disassembly
Nrf2 is a transcription factor playing an essential role in stress response. A knowledge of the significance of Nrf2 in cell function has not, as yet, reached out beyond transactivation of gene expression. This paper shows that GDF-15 and SDF-1-induced angiogenesis strongly depends on Nrf2 presence,...
ORGANISM(S): Homo sapiens (Human) 
2018-11-28 | PXD009161 | Pride
Cells Human 
ProteomicsKeap1 governs ageing-induced protein aggregation in endothelial cells
The breach of proteostasis, leading to the accumulation of protein aggregates, is a hallmark of ageing and age-associated disorders, up to now well-established in neurodegeneration. Few studies have addressed the issue of dysfunctional cell response to protein deposition also for the cardiovascular ...
ORGANISM(S): Homo sapiens (Human) 
2020-08-12 | PXD018969 | Pride
Aggregates Keap1 Human 
ProteomicsKeap-1-mediated S-nitrosylation of NOX4 hampers oxidative damage in endothelium
ABSTRACT Rationale Premature senescence is conducive to aging and cardiovascular diseases. Nrf2 transcription factor, the master orchestrator of adoptive response to cellular stress, has been implicated in regulation of premature senescence in fibroblasts, neural and mesenchymal stem cells by trans...
ORGANISM(S): Homo sapiens (Human) 
2020-08-12 | PXD013640 | Pride
Endothelial cells S-nitrosylation Nrf2 Nox4 Oxidative stress 
ProteomicsSelective autophagy cargo receptor NBR1 is involved in plant response to sulfur deficit
Autophagy involvement in plant response to nitrogen, carbon and sulfur starvation was already reported, however the mechanisms responsible for its regulation and selectivity in such conditions were not yet investigated. We observed increased amounts of NBR1 transcript in plants exposed to sulfur def...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-26 | PXD016026 | Pride
Nbr1 Arabidopsis 
ProteomicsHuman dihydrofolate reductase is a substrate of protein kinase CK2α
Dihydrofolate reductase (DHFR) is a prominent molecular target in antitumor, antibacterial and antiprotozoan chemotherapies. Our in silico amino acid sequence and 3D structure analyses revealed the presence of several putative CK2 phosphorylation sites. Indeed, CK2α subunit phosphorylated DHFR in v...
ORGANISM(S): Homo sapiens (Human) 
2019-04-15 | PXD011853 | Pride
Lc-ms/ms Ck2 : dihydrofolate reductase Protein phosphorylation Protein mutants Phosphoproteomics 
ProteomicsIdentification of proteins differentially regulated upon perturbed mitochondrial gene expression
The aim of this project was to search for the new factors engaged in mitochondrial nucleic acids metabolism under stress conditions. To this end we treated human 293 cells with mitochondrial replication/transcription inhibitor ethidium bromide and we compared proteomes of untreated vs treated cells....
ORGANISM(S): Homo sapiens (Human) 
2019-06-13 | PXD012055 | Pride
Mitochondria Lc-ms/ms 
ProteomicsThe non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma
FAM46C is one of the most frequently mutated genes in multiple myeloma (MM). Here, using a combination of in vitro and in vivo approaches, we demonstrate that FAM46C encodes an active non-canonical poly(A) polymerase which enhances mRNA stability and gene expression. Reintroduction of active FAM46C ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-09 | PXD006790 | Pride
Lc-msms Fam46c Anemia Mouse B lymphocytes Polyadenylation Multiple myeloma 
ProteomicsProteom-wide analysis of lysine N-homocysteinylation and Hcy-induced proteom changes in Saccharomyces cerevisiae - SILAC data
Hyperhomocysteinemia (HHcy) inhibits growth and is cytotoxic to bacterial, yeast, and mammalian cells. The aim of this study was to determine the changes in proteome of the yeast induced by HHcy and map N-homocysteinylated sites. We identified 38 up- and 32-down-regulated proteins as well as 244 N-h...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-16 | PXD019951 | Pride
Proteomics Lc-ms Silac N-homocysteinylation Yeast 
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