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ProteomicsHydrogen-deuterium exchange mass spectrometry of wt Mtr4 with various RNA substrates
Mtr4 is a eukaryotic RNA helicase required for RNA decay by the nuclear exosome. Previous studies have shown how RNA enroute to the exosome threads through the highly conserved helicase core of Mtr4. Mtr4 also contains an arch domain, although details of potential interactions between the arch and R...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-07 | PXD031962 | Pride
Hdx Helicase Hdx-ms Mtr4 Bottom-up hdx 
ProteomicsHistone variant H2A.Z enhances histone and nucleosome dynamics
Interchanging canonical histone H2A with variant H2A.Z in chromatin complexes is vital for the proper regulation of transcription, DNA damage repair, and centromere maintenance. However, the physical mechanisms underlying functional differences between H2A and H2A.Z complexes are unclear. Human H2A ...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2025-12-29 | PXD062956 | Pride
Molecular dynamics Hdx-ms Chromatin regulation Histone variants 
ProteomicsDistinct mechanistic, conformational and substrate selectivity profiles emerging in the evolution of high catalytic activity enzymes via parallel trajectories
Recent laboratory evolution studies have demonstrated that parallel evolutionary trajectories can lead to genetically distinct enzymes with high activity towards a non-preferred substrate. However, it is unknown whether such genetically distinct enzymes have convergent conformational dynamics and me...
ORGANISM(S): Homo sapiens (Human) 
2024-07-29 | PXD053893 | Pride
Kynureninase Enzyme Substrate Hdx Engineering 
ProteomicsMORC2 phosphorylation fine tunes its DNA compaction activity
Variants in the poorly characterized oncoprotein, MORC2, a chromatin remodeling ATPase, lead to defects in epigenetic regulation and DNA damage response. MORC2 variants are associated with multiple cancers and neurological disorders. The C-terminal domain (CTD) of MORC2is often phosphorylated in DNA...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD053379 | Pride
Atpase Morc2 Single molecule Cryo-em Crosslinking ms Flim-fret Hdx-ms Epigenetics Ffs Chromatin remodeling 
ProteomicsHDX-MS of the TRAMP Complex with tRNA
TRAMP is a eukaryotic protein complex with RNA helicase and polyadenylase activity that marks and delivers a variety of RNA substrates to the nuclear exosome for degradation. Prior work has characterized binding of Trf4 and Air2 to the helicase core of Mtr4 and the regulation of both enzymatic activ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-12-11 | PXD053773 | Pride
Hdx Trf4 Tramp Air2 Rna Hdx-ms Mtr4 
ProteomicsMolecular basis of RanGTP-activated release of Histones H2A-H2B from Importin-9
Imp9 is the primary importin for shuttling H2A-H2B from the cytoplasm to the nucleus. It employs an unusual mechanism where the binding of RanGTP is insufficient to release H2A-H2B. The resulting stable RanGTP•Imp9•H2A-H2B complex gains nucleosome assembly activity with H2A-H2B able to be deposited ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog) 
2023-06-02 | PXD037571 | Pride
H2a-h2b Cryo-em Kap114 Importin-9 Rangtp Hdx-ms 
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