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Proteomics17β-Estradiol Delivered in Eye Drops: A Proteomics Study Focusing on the Rat Retina
Five ovariectomized (OVX) Brown Norway rats (Charles Rivers Laboratories, Wilmington, DE, USA) weighing 200-250 g received 10 µL of 17β-estradiol (E2) eye drops once daily in both eyes for three weeks [20]. The eye drops contained 0.1% (w/v) E2 in saline vehicle containing 20% (w/v) 2-hydroxypropyl-...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-05-27 | PXD010851 | Pride
Retina Datadependent acquisition Rat Label-free Estradiol 
ProteomicsOptimized Sample Preparation Workflow for Improved Identification of Ghost Proteins
Large scale proteomic strategies rely on database interrogation. Thus, only referenced proteins can be identified. Recently, Alternative Proteins (AltProts) translated from nonannotated Alternative Open reading frame (AltORFs) were discovered using customized databases. Because of their small size w...
ORGANISM(S): Homo sapiens (Human) 
2021-02-15 | PXD024134 | Pride
Protein identification Fractionation Precipitation Peptides and proteins Extraction 
ProteomicsN1-Methylpseudouridine and pseudouridine modifications modulate mRNA decoding during translation
Mass spectrometry on affinity-purified luciferase produced from constitutively modified or unmodified mRNA in HEK293 cells.
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD053919 | Pride
N1-methylpseudouridine Ribosome Translation 
ProteomicsCancer Associated Fibroblast-FAK regulates malignant cell metabolism
Breast and pancreatic cancers are characterised by profound metabolic changes1,2. Until recently, these changes have been ascribed to their intrinsic genetic profiles2-4. However, the contribution of cancer-associated fibroblasts (CAFs) to cell metabolism has not been fully investigated5. Here, we p...
ORGANISM(S): Mus musculus (Mouse) 
2020-02-18 | PXD008276 | Pride
Fak Metabolism Fibroblast 
ProteomicsMS analysis of GAR1 methylation immunopurified from HEK-293 Cells
The conserved box H/ACA RNPs consist of one box H/ACA RNA and 4 core proteins: Dyskerin, NHP2, NOP10 and GAR1. The assembly of the H/ACA RNPs is a stepwise process which requires several assembly factors: SHQ1, NAF1, the Survival of Motor Neuron complex SMN, and the R2TP complex. It implicates the c...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD026752 | Pride
Methylation Apms 
ProteomicsComparative mass spectrometry reveals a novel TTC33-associated network core (TANC) complex with functions in DNA damage response
TTC33 is a 30 kDa human uncharacterized tetratricopeptide repeat protein. Here, we employed label-free mass spectrometry analysis to identify the proteins interactors. The TTC33-associated network (TAN) is composed of WDR61, PHF5A, which together with TTC33 form a trimeric core complex (TANC), that ...
ORGANISM(S): Homo sapiens (Human) 
2026-07-06 | PXD059336 | Pride
Ttc33 Dna repair Wdr61 Pafc Ung dna glycosylase Skic Phf5a 
ProteomicsEssential PBP1-associated proteins of Trypanosoma brucei
Control of gene expression in kinetoplastids depends heavily on RNA-binding proteins that influence mRNA decay and translation. We previously showed that MKT1 interacts with PBP1, which in turn recruits LSM12 and poly(A) binding protein. MKT1 is recruited to mRNA by sequence-specific RNA-binding pro...
ORGANISM(S): Trypanosoma brucei 
2020-07-14 | PXD018576 | Pride
Trypanosoma Pbp1 Ataxin-2 Poly(a) binding protein Mkt1 Mrna 
ProteomicsProteins associated wth Trypanosoma brucei HNRNPF/H
We aimed to find proteins associated with HNRNPH in bloodstream-form _Trypanosoma brucei_. We integrated a sequence encoding a cleavable TAP (Tandem Affinity Purification) tag into the genome upstream of, and in frame with, one _HNRNPH_ gene. The other allele was deleted. TAP-HNRNPH was purifed fro...
ORGANISM(S): Trypanosoma brucei 
2022-10-15 | PXD037289 | Pride
Trypanosoma brucei  mrna  rna-binding protein  trans splicing  mrna decay 
ProteomicsIntracellular Energy Controls Dynamics of Stress-induced Ribonucleoprotein Granules Implicated in ALS/FTD
Energy metabolism and membraneless organelles have been implicated in human diseases including neurodegeneration. How energy deficiency regulates ribonucleoprotein particles such as stress granules (SGs) is still unclear. Here we identified a unique type of granules formed under energy deficiency an...
ORGANISM(S): Homo sapiens (Human) 
2022-10-15 | PXD035747 | Pride
Stress granule  energy metabolism  als/ftd  protein homeostasis 
ProteomicsSILAC APMS analysis of GFP tagged TSC2 in Hela Cells
HSP90 and its co-chaperon R2TP are involved to assemble and stabilize many macro-molecular complexes involved in cell proliferation. R2TP complex is composed of RUVBL1 and RUVBL2 AAA+ ATPases and two adapter proteins RPAP3 and PIH1D1. The N-terminal domain of PIH1D1 was described to possess a basic ...
ORGANISM(S): Homo sapiens (Human) 
2024-11-12 | PXD030402 | Pride
Immunoprecipitation Silac Gfp 
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