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ProteomicsDNA pulldown of PRDM16 promoter and measurement of LC/MS-MS
To further investigate the transcription cofactor of Smad3 that regulated the transcription of PRDM16, we conducted DNA pull-down assay. we treated HK-2 cells with TGF-β and extracted the nuclear proteins. And the nuclear proteins were incubated with streptavidin magnetic beads, and biotin-labeled D...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD044753 | Pride
Hk-2 cell line dna pull-down lc/ms-ms 
ProteomicsDNA pull-down and mass spectrometry
DNA pull-down and mass spectrometry analysis were performed by utilizing a biotin-labeled human PDPN promoter.
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD067543 | Pride
Human nasal epithelial cells Coomassie brilliant blue staining Lc-ms Dna pulldown 
ProteomicsChemoproteomic profiling to identify activity changes and functional inhibitors of DNA-binding proteins
DNA-binding proteins are promising therapeutic targets but notoriously difficult to drug. Here, we evaluate a chemoproteomic DNA interaction platform as a complementary strategy for parallelized compound profiling. To enable this approach, we determined the proteomic binding landscape of 92 immobili...
ORGANISM(S): Homo sapiens (Human) 
2023-02-03 | PXD037297 | Pride
Reactive side chain profiling Mass spectrometry Drug screening Pull-down Covalency Dna binding Chemical proteomics 
ProteomicsPull-down affinity purifications using di-nucleosomes decorated with enhancer-associated modifications and incorporating 200bp linker DNA
Characteristic features of chromatin states are not limited to particular epigenetic modifications but include other regulatory cues, such as linker DNA length, typically ranging from around 35-55 bp in most eu- and heterochromatin domains (Valouev et al, 2011; Voong et al., 2016, Cell) to over 200 ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-12 | PXD041443 | Pride
Ndrs H3k4me1 H3k27ac Pull-down 
ProteomicsDissecting the effect of a long linker DNA on protein binding to di-nucleosomes decorated with promoter-associated PTMs
Characteristic features of chromatin states are not limited to particular epigenetic modifications but include other regulatory cues, such as linker DNA length, typically ranging from around 35-55 bp in most eu- and heterochromatin domains (Valouev et al, 2011; Voong et al., 2016, Cell) to over 200 ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-10 | PXD041835 | Pride
Ndrs Sv40 promoter H3k4me3 Pull-down H4ac H3ac 
ProteomicsProteomics Studies of the Interactome of RNA Polymerase II C-Terminal Repeated Domain
We developed a proteomics approach to examine the mammalian CTD-interactome. We used six synthetic peptides each consisting of four consensus CTD-repeats and with different combinations of serine and tyrosine phosphorylation as affinity-matrix to pull-down nuclear proteins from HeLa cells. The pull-...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000078867 | MassIVE
ProteomicsPALB2 ChAM acetylation mapping in human cells
The Chromatin Association Motif (ChAM) of the PArtner and Localizer of BRCA2 (PALB2) is essential for DNA repair function by homologous recombination in human cells. Molecular mechanisms governing ChAM interaction with chromatin remain unexplored. In this study, we have mapped acetylated lysines wit...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD014678 | Pride
Acetylation Dna repair Chromatin 
ProteomicsGlmS plays a key role in the virulence and biofilm formation ability of Staphylococcus aureus promoted by advanced glycation end products
Staphylococcus aureus (S. aureus) is well known for its biofilm formation ability and is responsible for serious, chronic refractory infections worldwide. We previously demonstrated that advanced glycation end products (AGEs), a hallmark of chronic hyperglycemia in diabetic tissues, enhanced biofilm...
ORGANISM(S): Staphylococcus aureus 
2024-05-22 | PXD046076 | Pride
Staphylococcus aureus  advanced glycation end products  biofilm formation  glms-sigb regulatory axis 
ProteomicsPulldown proteomics of XybR in SirexAA-E
Streptomyces sp. SirexAA-E chnages secretion of enzymes that can degrade xylan. These enzymes transcription is regulated by a transcriptional regulator. To determine the dominant regulator, we performed pulldown proteomics, using some promoter regions (P0265, P0357, P5230) which are related to xylan...
ORGANISM(S): Streptomyces sp. SirexAA-E 
2025-11-11 | PXD061680 | Pride
Pulldown proteomics Xylan Hemicellulose Transcriptonal regulator 
ProteomicsTherapeutic Targeting of AREL1 in Hepatic Stellate Cells Attenuates MASH-related Liver Fibrosis
This submission includes two proteomics datasets: (1) DNA pull-down using the biotinylated promoter region of AREL1 to identify promoter-interacting proteins; (2) immunoprecipitation of FLAG-tagged AREL1 followed by mass spectrometry to identify AREL1 protein interactors. Both experiments were perfo...
ORGANISM(S): Homo Sapiens 
PXD078783 |
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