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UnknownThiol-exchange in DTSSP crosslinked peptides is proportional to cysteine content and precisely controlled in crosslink detection by two-step LC-MALDI MSMS.
Not available
S-EPMC3218361 | biostudies-literature
ProteomicsCross-linking Analysis (HSP70-HSP90-CHIP)
Some molecular chaperones are involved not only in assisting the folding of proteins but also, given appropriate conditions, in their degradation. This is the case of Hsp70 and Hsp90, which in concert with the cochaperone CHIP –an E3 ligase–, direct their bound substrate to degradation through ubiqu...
ORGANISM(S): Homo sapiens (Human) 
2019-03-06 | PXD009777 | Pride
Hsp90 Dtssp Hsp70 Chip 
ProteomicsInteraction of saposin C and acid-beta-glucosidase
The objective of this project was to determine the interaction interface(s) between acid beta-glucosidase (GCase) and its activator protein saposin C (SapC). Chemical crosslinking was used to confirm the formation of discrete heterodimers between the two proteins, using lysine-reactive crosslinkers ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD058056 | Pride
Gaucher Lysosome Glucosidase Saposin Crosslinking 
ProteomicsGIPC3 AP-MS/MS from chick inner ear extracts
To understand how GIPC3 exerts its effects on cuticular plate dimensions, we examined the GIPC3 protein-interaction network in hair cells. We immunoaffinity purified GIPC3 from crosslinked chicken inner ear extracts that were enriched for stereocilia, but still contain large amounts of hair-cell cyt...
ORGANISM(S): Gallus gallus (Chicken) 
2023-05-09 | PXD038234 | Pride
Stereocilia Myosin Cytoskeleton Hair cell Actin Hair bundle 
UnknownDataset on interactors of the Arabidopsis thaliana Plant Natriuretic Peptide (AtPNP-A) determined by mass spectrometry.
Not available
S-EPMC7210391 | biostudies-literature
UnknownEffects of freezing and protein cross-linker on isolating membrane raft-associated proteins.
Not available
S-EPMC3122141 | biostudies-literature
ProteomicsPull-down assay coupled with mass spectrometry using Vstm2b-Fc and rat primary hippocampal neurons
This study aimed to identify Vstm2b-interacting proteins in rat primary hippocampal neurons cultured for 14 days in vitro. Neurons were incubated with Vstm2b-Fc– or Fc-coated Protein G magnetic beads and crosslinked with DTSSP. Following cell lysis, bound proteins were isolated by magnetic separatio...
ORGANISM(S): Rattus Rattus (black Rat) 
2026-08-12 | PXD079896 | JPOST Repository
UnknownProtein nanoparticle vaccine based on flagellin carrier fused to influenza conserved epitopes confers full protection against influenza A virus challenge.
Not available
S-EPMC5526067 | biostudies-literature
UnknownVersatile Reversible Cross-Linking Strategy to Stabilize CCMV Virus Like Particles for Efficient siRNA Delivery.
Not available
S-EPMC6923791 | biostudies-literature
TranscriptomicsSingle-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice
This repository contains single-nucleus gene expression profiling from mouse livers of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or...
ORGANISM(S): Mus musculus 
2024-10-15 | E-MTAB-13700 | biostudies-arrayexpress
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