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MetabolomicsComparison of popular enrichment methods for untargeted in vitro metabolomics.

Introduction

Untargeted metabolomics is a popular method by which researchers measure a large portion of the metabolites present in a biological system at once. This approach usually results in complex data sets containing tens to hundreds of thousands of observations whi...

2025-08-11 | MTBLS12332 | MetaboLights
ProteomicsComparison of deep plasma proteome enrichment methods
Plasma is a complex biological fluid containing extracellular vesicles (EVs), residual platelets, and soluble proteins, all of which can serve as biomarkers for pathological conditions. While conventional plasma proteomics typically identifies hundreds of proteins, recent enrichment strategies have ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD063673 | Pride
Enrichplus Proteograph Magnet Timstof Enrichment Plasma Evs Lc-ms/ms 
ProteomicsFilter-aided extracellular vesicle enrichment (FAEVEr) for proteomics
Comaprison of the extracellular vesicle proteome using Filter-Aided Extracellular vescile Enrichment (FAEVEr) and ultracentrifugation. During enrichment different percentages of TWEEN-20 were supplemented in the wash buffer.
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-05-06 | PXD051938 | Pride
Ultracentrifugation Ev enrichment Optimization Ultrafiltration 
ProteomicsComparison of the MCF7 proteome cultured in standard FBS and EV depleted FBS
COmparison of the MCF7 proteomes after being cultured for 3 d in either 10% FBS (standard, commercial) or in 10% EV-depleted FBS (EDS, commercial) to validate the absence of EV specific proteins in EDS to exclude potential cross-contamination of xeno-EVs
ORGANISM(S): Bos taurus (Bovine) 
2025-05-06 | PXD051956 | Pride
Ev-depleted fbs Extracellular vesicles Starvation Fbs 
ProteomicsSecretome containing extracellular vesicles (EV) from cardiovascular progenitors (EV-CPC) vs the secretome containing EV from Fibroblasts (EV-FB)
Secretome containing extracellular vesicles (EV) seem to mediate the benefits of cell therapy for ischemic heart failure. Our project has the objective of comparing the secretome containing extracellular vesicles (EV) from cardiac progenitor cells (EV-CPC) vs the secretome containing EV from Fibrobl...
ORGANISM(S): Homo sapiens (Human) 
2023-09-22 | PXD022129 | Pride
Extracellular vesicles (ev) Fibroblasts Secretome from cardic progenitors Cardiovascular progenitor cells (cpc) 
ProteomicsChronic Perinatal Oxycodone Exposure Alters Placental EV Proteome and Fetal Cardiomyopathy-Linked Pathways
The rising prevalence of opioid use during pregnancy poses serious public health concerns, yet the molecular mechanisms by which opioids such as oxycodone impact fetal development remain poorly understood. This study investigates the effects of chronic perinatal oxycodone exposure on the composition...
ORGANISM(S): Rattus norvegicus (Rat) 
2026-04-27 | PXD065451 | Pride
Ev biomarkers Oxycodone Perinatal exposure Cardiomyopathy Placental signaling Proteomics Fetal development Extracellular vesicles (evs) 
TranscriptomicsExperimental identification of apoptosis-associated miR-21 target genes in Jurkat T-cells using a high-throughput experimental approach
MiR-21 is an important suppressor of T-cell apoptosis that is also widely overexpressed in many types of cancers. The exact mechanisms related to the anti-apoptotic effect of miR-21 is not well understood. In this study, we applied AGO2 RNA Immunoprecipitation followed by gene expression profiling (...
ORGANISM(S): Homo sapiens 
2016-08-03 | E-GEOD-85116 | biostudies-arrayexpress
ProteomicsOptimising size exclusion chromatography for extracellular vesicle enrichment and proteomic analysis from clinically relevant samples
We performed a quantitative evaluation and optimization of SEC for separating EVs from contaminating proteins. This optimized method was applied to enrich EVs from healthy plasma and MDA-MB-231 cancer cell culture medium. By spiking-in cancer cell-derived EVs to healthy plasma and then performing SE...
ORGANISM(S): Homo sapiens (Human) 
2019-01-16 | PXD010341 | Pride
Plasma Chromatography Extracellular vesicle 
TranscriptomicsExtracellular Vesicles from patients with Antibody-Mediated Rejection induce tubular senescence and endothelial to mesenchymal transition by regulating complement activation through horizontal transfer of miRNAs
Extracellular vesicles (EV) has been shown to deliver potential microRNA (miRNA) as cargo for specific target cells; effectively, the EV unique nature of the bilayer allows miRNA to be protected from degradation. We used microarray analysis to compare the miRNA profiles of EV isolated from acute ant...
ORGANISM(S): Homo sapiens 
2021-09-10 | E-MTAB-10447 | biostudies-arrayexpress
UnknownEV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations.
Not available
S-EPMC11653091 | biostudies-literature
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